<i>Gentiana lutea</i> Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes
<i>Gentiana lutea</i> is a bitter herb that is traditionally used to improve gastric disorders. Recently, we have shown that <i>Gentiana lutea</i> extract (GE) also modulates the lipid metabolism of human keratinocytes in vitro and in vivo. In the present study, we investigat...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/8/1832 |
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| author | Fabian Gendrisch Anna Nováčková Michaela Sochorová Birgit Haarhaus Kateřina Vávrová Christoph M. Schempp Ute Wölfle |
| author_facet | Fabian Gendrisch Anna Nováčková Michaela Sochorová Birgit Haarhaus Kateřina Vávrová Christoph M. Schempp Ute Wölfle |
| author_sort | Fabian Gendrisch |
| collection | DOAJ |
| description | <i>Gentiana lutea</i> is a bitter herb that is traditionally used to improve gastric disorders. Recently, we have shown that <i>Gentiana lutea</i> extract (GE) also modulates the lipid metabolism of human keratinocytes in vitro and in vivo. In the present study, we investigated the role of GE on ceramide synthesis in human primary keratinocytes (HPKs) and psoriasis-like keratinocytes. We could demonstrate that GE increased the concentrations of glucosylceramides and the ceramide AS/AdS subclass without affecting the overall ceramide content in HPKs. The expression of ceramide synthase 3 (<i>CERS3</i>) and elongases (<i>ELOVL1</i> and <i>4</i>) was reduced in psoriasis lesions compared to healthy skin. Psoriasis-like HPKs, generated by stimulating HPKs with cytokines that are involved in the pathogenesis of psoriasis (IL-17, TNF-α, IL-22 and IFN-γ) showed increased levels of IL-6, IL-8 and increased expression of <i>DEFB4A</i>, as well as decreased expression of <i>ELOVL4</i>. The treatment with GE partly rescued the reduced expression of <i>ELOVL4</i> in psoriasis-like HPKs and augmented <i>CERS3</i> expression. This study has shown that GE modulates ceramide synthesis in keratinocytes. Therefore, GE might be a novel topical treatment for skin diseases with an altered lipid composition such as psoriasis. |
| first_indexed | 2024-03-10T20:25:41Z |
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| last_indexed | 2024-03-10T20:25:41Z |
| publishDate | 2020-04-01 |
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| series | Molecules |
| spelling | doaj.art-f1583c43ea7a4abdbd6f5690c77757582023-11-19T21:49:51ZengMDPI AGMolecules1420-30492020-04-01258183210.3390/molecules25081832<i>Gentiana lutea</i> Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like KeratinocytesFabian Gendrisch0Anna Nováčková1Michaela Sochorová2Birgit Haarhaus3Kateřina Vávrová4Christoph M. Schempp5Ute Wölfle6Department of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Breisgau, GermanySkin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, CZ-50005 Hradec Králové, Czech RepublicSkin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, CZ-50005 Hradec Králové, Czech RepublicDepartment of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Breisgau, GermanySkin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Akademika Heyrovského 1203, CZ-50005 Hradec Králové, Czech RepublicDepartment of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Breisgau, GermanyDepartment of Dermatology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Breisgau, Germany<i>Gentiana lutea</i> is a bitter herb that is traditionally used to improve gastric disorders. Recently, we have shown that <i>Gentiana lutea</i> extract (GE) also modulates the lipid metabolism of human keratinocytes in vitro and in vivo. In the present study, we investigated the role of GE on ceramide synthesis in human primary keratinocytes (HPKs) and psoriasis-like keratinocytes. We could demonstrate that GE increased the concentrations of glucosylceramides and the ceramide AS/AdS subclass without affecting the overall ceramide content in HPKs. The expression of ceramide synthase 3 (<i>CERS3</i>) and elongases (<i>ELOVL1</i> and <i>4</i>) was reduced in psoriasis lesions compared to healthy skin. Psoriasis-like HPKs, generated by stimulating HPKs with cytokines that are involved in the pathogenesis of psoriasis (IL-17, TNF-α, IL-22 and IFN-γ) showed increased levels of IL-6, IL-8 and increased expression of <i>DEFB4A</i>, as well as decreased expression of <i>ELOVL4</i>. The treatment with GE partly rescued the reduced expression of <i>ELOVL4</i> in psoriasis-like HPKs and augmented <i>CERS3</i> expression. This study has shown that GE modulates ceramide synthesis in keratinocytes. Therefore, GE might be a novel topical treatment for skin diseases with an altered lipid composition such as psoriasis.https://www.mdpi.com/1420-3049/25/8/1832ceramidesskin<i>Gentiana lutea</i>CerSELOVL |
| spellingShingle | Fabian Gendrisch Anna Nováčková Michaela Sochorová Birgit Haarhaus Kateřina Vávrová Christoph M. Schempp Ute Wölfle <i>Gentiana lutea</i> Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes Molecules ceramides skin <i>Gentiana lutea</i> CerS ELOVL |
| title | <i>Gentiana lutea</i> Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes |
| title_full | <i>Gentiana lutea</i> Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes |
| title_fullStr | <i>Gentiana lutea</i> Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes |
| title_full_unstemmed | <i>Gentiana lutea</i> Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes |
| title_short | <i>Gentiana lutea</i> Extract Modulates Ceramide Synthesis in Primary and Psoriasis-Like Keratinocytes |
| title_sort | i gentiana lutea i extract modulates ceramide synthesis in primary and psoriasis like keratinocytes |
| topic | ceramides skin <i>Gentiana lutea</i> CerS ELOVL |
| url | https://www.mdpi.com/1420-3049/25/8/1832 |
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