Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China
A total of 38 strains were initially isolated from Jiaoke, a homemade traditional fermented cream, which was sourced from Inner Mongolia, China, and 6 strains were selected after screening, which was based on their cholesterol removal abilities. The Lactobacillus plantarum with the highest level of...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2011-06-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201103-0003_cholesterol-removal-by-lactobacillus-plantarum-isolated-from-homemade-fermented-cream-in-inner-mongolia-of-chin.php |
| _version_ | 1797899781141430272 |
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| author | Li-Dong Guo Li-Jie Yang Gui-Cheng Huo |
| author_facet | Li-Dong Guo Li-Jie Yang Gui-Cheng Huo |
| author_sort | Li-Dong Guo |
| collection | DOAJ |
| description | A total of 38 strains were initially isolated from Jiaoke, a homemade traditional fermented cream, which was sourced from Inner Mongolia, China, and 6 strains were selected after screening, which was based on their cholesterol removal abilities. The Lactobacillus plantarum with the highest level of cholesterol removal from the media was identified using phenotypical characteristics and 16S rRNA sequences, and was named L. plantarum KLDS 1.0344. The mechanisms for cholesterol removal involved co-precipitation, assimilation, and degradation of cholesterol by L. plantarum KLDS 1.0344. At a level of 8.56 log cfu/ml, L. plantarum KLDS 1.0344 survived in pH 2.5 MRS broth for 2 h and exhibited excellent tolerance to 0.3% (w/v) bile. This strain has the most potential in applications as a dietary supplement for lowering human serum cholesterol. A series of tests on animal model and/or clinical tests will be conducted before it can be used in a pharmaceutical application. |
| first_indexed | 2024-04-10T08:35:27Z |
| format | Article |
| id | doaj.art-f15c970b22c84fa797d14550263a8ebf |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:27Z |
| publishDate | 2011-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-f15c970b22c84fa797d14550263a8ebf2023-02-23T03:27:39ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172011-06-0129321922510.17221/224/2009-CJFScjf-201103-0003Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of ChinaLi-Dong Guo0Li-Jie Yang1Gui-Cheng Huo2Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaKey Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaKey Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaA total of 38 strains were initially isolated from Jiaoke, a homemade traditional fermented cream, which was sourced from Inner Mongolia, China, and 6 strains were selected after screening, which was based on their cholesterol removal abilities. The Lactobacillus plantarum with the highest level of cholesterol removal from the media was identified using phenotypical characteristics and 16S rRNA sequences, and was named L. plantarum KLDS 1.0344. The mechanisms for cholesterol removal involved co-precipitation, assimilation, and degradation of cholesterol by L. plantarum KLDS 1.0344. At a level of 8.56 log cfu/ml, L. plantarum KLDS 1.0344 survived in pH 2.5 MRS broth for 2 h and exhibited excellent tolerance to 0.3% (w/v) bile. This strain has the most potential in applications as a dietary supplement for lowering human serum cholesterol. A series of tests on animal model and/or clinical tests will be conducted before it can be used in a pharmaceutical application.https://cjfs.agriculturejournals.cz/artkey/cjf-201103-0003_cholesterol-removal-by-lactobacillus-plantarum-isolated-from-homemade-fermented-cream-in-inner-mongolia-of-chin.phpmechanismcholesterollactobacilluscream |
| spellingShingle | Li-Dong Guo Li-Jie Yang Gui-Cheng Huo Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China Czech Journal of Food Sciences mechanism cholesterol lactobacillus cream |
| title | Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China |
| title_full | Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China |
| title_fullStr | Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China |
| title_full_unstemmed | Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China |
| title_short | Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in Inner Mongolia of China |
| title_sort | cholesterol removal by lactobacillus plantarum isolated from homemade fermented cream in inner mongolia of china |
| topic | mechanism cholesterol lactobacillus cream |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201103-0003_cholesterol-removal-by-lactobacillus-plantarum-isolated-from-homemade-fermented-cream-in-inner-mongolia-of-chin.php |
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