Bioethanol Production from Cachaza as Hydrogen Feedstock: Effect of Ammonium Sulfate during Fermentation
Cachaza is a type of non-centrifugal sugarcane press-mud that, if it is not employed efficiently, generates water pollution, soil eutrophication, and the spread of possible pathogens. This biomass can be fermented to produce bioethanol. Our intention is to obtain bioethanol that can be catalytically...
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MDPI AG
2017-12-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/10/12/2112 |
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| author | Nestor Sanchez Ruth Yolanda Ruiz Nicolas Infante Martha Cobo |
| author_facet | Nestor Sanchez Ruth Yolanda Ruiz Nicolas Infante Martha Cobo |
| author_sort | Nestor Sanchez |
| collection | DOAJ |
| description | Cachaza is a type of non-centrifugal sugarcane press-mud that, if it is not employed efficiently, generates water pollution, soil eutrophication, and the spread of possible pathogens. This biomass can be fermented to produce bioethanol. Our intention is to obtain bioethanol that can be catalytically reformed to produce hydrogen (H2) for further use in fuel cells for electricity production. However, some impurities could negatively affect the catalyst performance during the bioethanol reforming process. Hence, the aim of this study was to assess the fermentation of Cachaza using ammonium sulfate ((NH4)2SO4) loadings and Saccharomyces cerevisiae strain to produce the highest ethanol concentration with the minimum amount of impurities in anticipation of facilitating further bioethanol purification and reforming for H2 production. The results showed that ethanol production from Cachaza fermentation was about 50 g·L−1 and the (NH4)2SO4 addition did not affect its production. However, it significantly reduced the production of branched alcohols. When a 160 mg·L−1 (NH4)2SO4 was added to the fermentation culture, 2-methyl-1-propanol was reduced by 41% and 3-methyl-1-butanol was reduced by 6%, probably due to the repression of the catabolic nitrogen mechanism. Conversely, 1-propanol doubled its concentration likely due to the higher threonine synthesis promoted by the reducing sugar presence. Afterwards, we employed the modified Gompertz model to fit the ethanol, 2M1P, 3M1B, and 1-propanol production, which provided acceptable fits (R2 > 0.881) for the tested compounds during Cachaza fermentation. To the best of our knowledge, there are no reports of the modelling of aliphatic production during fermentation; this model will be employed to calculate yields with further scaling and for life cycle assessment. |
| first_indexed | 2024-04-13T01:06:03Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1073 |
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| last_indexed | 2024-04-13T01:06:03Z |
| publishDate | 2017-12-01 |
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| record_format | Article |
| series | Energies |
| spelling | doaj.art-f15d831125694f20a6494078bb86e5642022-12-22T03:09:21ZengMDPI AGEnergies1996-10732017-12-011012211210.3390/en10122112en10122112Bioethanol Production from Cachaza as Hydrogen Feedstock: Effect of Ammonium Sulfate during FermentationNestor Sanchez0Ruth Yolanda Ruiz1Nicolas Infante2Martha Cobo3Energy, Materials, and Environmental Laboratory, Department of Chemical Engineering, Universidad de La Sabana, Campus Universitario Puente del Común, Km. 7 Autopista Norte, Bogotá 25001, ColombiaAgroindustrial Process Laboratory, Agroindustrial Process Engineering, Universidad de La Sabana, Campus Universitario Puente del Común, Km. 7 Autopista Norte, Bogotá 25001, ColombiaAgroindustrial Process Laboratory, Agroindustrial Process Engineering, Universidad de La Sabana, Campus Universitario Puente del Común, Km. 7 Autopista Norte, Bogotá 25001, ColombiaEnergy, Materials, and Environmental Laboratory, Department of Chemical Engineering, Universidad de La Sabana, Campus Universitario Puente del Común, Km. 7 Autopista Norte, Bogotá 25001, ColombiaCachaza is a type of non-centrifugal sugarcane press-mud that, if it is not employed efficiently, generates water pollution, soil eutrophication, and the spread of possible pathogens. This biomass can be fermented to produce bioethanol. Our intention is to obtain bioethanol that can be catalytically reformed to produce hydrogen (H2) for further use in fuel cells for electricity production. However, some impurities could negatively affect the catalyst performance during the bioethanol reforming process. Hence, the aim of this study was to assess the fermentation of Cachaza using ammonium sulfate ((NH4)2SO4) loadings and Saccharomyces cerevisiae strain to produce the highest ethanol concentration with the minimum amount of impurities in anticipation of facilitating further bioethanol purification and reforming for H2 production. The results showed that ethanol production from Cachaza fermentation was about 50 g·L−1 and the (NH4)2SO4 addition did not affect its production. However, it significantly reduced the production of branched alcohols. When a 160 mg·L−1 (NH4)2SO4 was added to the fermentation culture, 2-methyl-1-propanol was reduced by 41% and 3-methyl-1-butanol was reduced by 6%, probably due to the repression of the catabolic nitrogen mechanism. Conversely, 1-propanol doubled its concentration likely due to the higher threonine synthesis promoted by the reducing sugar presence. Afterwards, we employed the modified Gompertz model to fit the ethanol, 2M1P, 3M1B, and 1-propanol production, which provided acceptable fits (R2 > 0.881) for the tested compounds during Cachaza fermentation. To the best of our knowledge, there are no reports of the modelling of aliphatic production during fermentation; this model will be employed to calculate yields with further scaling and for life cycle assessment.https://www.mdpi.com/1996-1073/10/12/2112agroindustrial wastesbiomassfermentation impuritiesrenewable energysugarcane |
| spellingShingle | Nestor Sanchez Ruth Yolanda Ruiz Nicolas Infante Martha Cobo Bioethanol Production from Cachaza as Hydrogen Feedstock: Effect of Ammonium Sulfate during Fermentation Energies agroindustrial wastes biomass fermentation impurities renewable energy sugarcane |
| title | Bioethanol Production from Cachaza as Hydrogen Feedstock: Effect of Ammonium Sulfate during Fermentation |
| title_full | Bioethanol Production from Cachaza as Hydrogen Feedstock: Effect of Ammonium Sulfate during Fermentation |
| title_fullStr | Bioethanol Production from Cachaza as Hydrogen Feedstock: Effect of Ammonium Sulfate during Fermentation |
| title_full_unstemmed | Bioethanol Production from Cachaza as Hydrogen Feedstock: Effect of Ammonium Sulfate during Fermentation |
| title_short | Bioethanol Production from Cachaza as Hydrogen Feedstock: Effect of Ammonium Sulfate during Fermentation |
| title_sort | bioethanol production from cachaza as hydrogen feedstock effect of ammonium sulfate during fermentation |
| topic | agroindustrial wastes biomass fermentation impurities renewable energy sugarcane |
| url | https://www.mdpi.com/1996-1073/10/12/2112 |
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