Genomic structure and phylogenetic analysis of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae)
The complete nucleotide sequence and the exon-intron structure of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) is described. The luciferase gene of the L. lateralis firefly spans 1,971 bp and consists of six introns and seven exons coding for 548 amino acid residues...
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| Format: | Article |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2004-03-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-200401-0001_Genomic_structure_and_phylogenetic_analysis_of_the_luciferase_gene_of_the_firefly_Luciola_lateralis_Coleopter.php |
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| author | Jong Gill KIM Yong Soo CHOI Keun Young KIM Jin Sik BAE Iksoo KIM Hung Dae SOHN Byung Rae JIN |
| author_facet | Jong Gill KIM Yong Soo CHOI Keun Young KIM Jin Sik BAE Iksoo KIM Hung Dae SOHN Byung Rae JIN |
| author_sort | Jong Gill KIM |
| collection | DOAJ |
| description | The complete nucleotide sequence and the exon-intron structure of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) is described. The luciferase gene of the L. lateralis firefly spans 1,971 bp and consists of six introns and seven exons coding for 548 amino acid residues. From samples collected at Boun and Muju, Korea, three isoforms, with identical exon-intron structure, named BU, MJ1 and MJ2, respectively, were obtained. Although the amino acid sequences of MJ1 and MJ2 were identical to those of known luciferase genes of Korean origin, the BU type was novel, differing from each of the MJ1 and MJ2 types by one amino acid. The luciferase sequences of the Korean samples, including those previously revealed, differed only by one - two amino acid residues, but differed by five - six amino acid residues from that of the luciferase gene recorded from specimens from Japan, which suggest genetic divergence has occurred in this species. Phylogenetic analyses using both amino acid and nucleotide sequences further showed that the luciferase gene of the Japanese L. lateralis firefly is genetically distinguishable from that of Korean L. lateralis, suggesting the presence of a genetic subdivision between the L. lateralis dwelling in the Korean Peninsula and Japanese Islands. |
| first_indexed | 2024-12-14T21:11:09Z |
| format | Article |
| id | doaj.art-f15dfeb6bdd34502bbd0b3a381b40fa1 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-14T21:11:09Z |
| publishDate | 2004-03-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-f15dfeb6bdd34502bbd0b3a381b40fa12022-12-21T22:47:16ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292004-03-01101111110.14411/eje.2004.001eje-200401-0001Genomic structure and phylogenetic analysis of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae)Jong Gill KIM0Yong Soo CHOI1Keun Young KIM2Jin Sik BAE3Iksoo KIM4Hung Dae SOHN5Byung Rae JIN6Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Suwon 441-100, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaDepartment of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Suwon 441-100, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaDepartment of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Suwon 441-100, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaThe complete nucleotide sequence and the exon-intron structure of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) is described. The luciferase gene of the L. lateralis firefly spans 1,971 bp and consists of six introns and seven exons coding for 548 amino acid residues. From samples collected at Boun and Muju, Korea, three isoforms, with identical exon-intron structure, named BU, MJ1 and MJ2, respectively, were obtained. Although the amino acid sequences of MJ1 and MJ2 were identical to those of known luciferase genes of Korean origin, the BU type was novel, differing from each of the MJ1 and MJ2 types by one amino acid. The luciferase sequences of the Korean samples, including those previously revealed, differed only by one - two amino acid residues, but differed by five - six amino acid residues from that of the luciferase gene recorded from specimens from Japan, which suggest genetic divergence has occurred in this species. Phylogenetic analyses using both amino acid and nucleotide sequences further showed that the luciferase gene of the Japanese L. lateralis firefly is genetically distinguishable from that of Korean L. lateralis, suggesting the presence of a genetic subdivision between the L. lateralis dwelling in the Korean Peninsula and Japanese Islands.https://www.eje.cz/artkey/eje-200401-0001_Genomic_structure_and_phylogenetic_analysis_of_the_luciferase_gene_of_the_firefly_Luciola_lateralis_Coleopter.phpfireflyluciola lateralisluciferase genegenomic structurephylogenetic analysis |
| spellingShingle | Jong Gill KIM Yong Soo CHOI Keun Young KIM Jin Sik BAE Iksoo KIM Hung Dae SOHN Byung Rae JIN Genomic structure and phylogenetic analysis of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) European Journal of Entomology firefly luciola lateralis luciferase gene genomic structure phylogenetic analysis |
| title | Genomic structure and phylogenetic analysis of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) |
| title_full | Genomic structure and phylogenetic analysis of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) |
| title_fullStr | Genomic structure and phylogenetic analysis of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) |
| title_full_unstemmed | Genomic structure and phylogenetic analysis of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) |
| title_short | Genomic structure and phylogenetic analysis of the luciferase gene of the firefly, Luciola lateralis (Coleoptera: Lampyridae) |
| title_sort | genomic structure and phylogenetic analysis of the luciferase gene of the firefly luciola lateralis coleoptera lampyridae |
| topic | firefly luciola lateralis luciferase gene genomic structure phylogenetic analysis |
| url | https://www.eje.cz/artkey/eje-200401-0001_Genomic_structure_and_phylogenetic_analysis_of_the_luciferase_gene_of_the_firefly_Luciola_lateralis_Coleopter.php |
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