Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress
In the bioremediation process, coexistence of lead (Pb) and cadmium causes complex toxicity, resulting in the difficulty of bioremediation. This study investigated the physiological responses and bioaccumulation mechanisms of the typical filamentous fungus Aspergillus niger under the coexistence of...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1096384/full |
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| author | Shang Pan Shang Pan Shang Pan Zhaoyan Li Jiayi Wang Xuefei Li Lingzi Meng Yunhui Chen Mu Su Zhen Li Zhen Li Zhen Li |
| author_facet | Shang Pan Shang Pan Shang Pan Zhaoyan Li Jiayi Wang Xuefei Li Lingzi Meng Yunhui Chen Mu Su Zhen Li Zhen Li Zhen Li |
| author_sort | Shang Pan |
| collection | DOAJ |
| description | In the bioremediation process, coexistence of lead (Pb) and cadmium causes complex toxicity, resulting in the difficulty of bioremediation. This study investigated the physiological responses and bioaccumulation mechanisms of the typical filamentous fungus Aspergillus niger under the coexistence of Pb and Cd. Four treatments were set up, i.e., control, sole Pb, sole Cd, and coexistence of Pb and Cd. The morphology of A. niger were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. Then, nano-scale secondary ion mass spectrometry (NanoSIMS) was applied to accurately investigate the distribution of heavy metals in the fungal cells under the coexistence of Pb and Cd. Finally, the metallogenic process and mineral types were simulated by Geochemist’s Workbench (GWB). The electron microscopic and NanoSIMS imaging showed that Pb and Cd were accumulated in both the extracellular and intracellular regions of the A. niger cells. In particular, the accumulated Pb content was ten times higher than that of Cd. However, Cd showed stronger toxicity than Pb to A. niger. Compared with the control treatment, Cd stress resulted in a two-fold increase of cell diameter and more extracellular substances, whereas the cell diameter increased nearly four times in the coexistence treatment. Moreover, the bioaccumulation of Pb was more intense than that of Cd during competitive sorption. The GWB simulation confirmed that Pb2+ can form multiple minerals (e.g., PbC2O4, PbHPO4, and Pb3(PO4)2, etc.), which significantly weakened its toxicity on the cell surface. This study elucidated the morphological characteristics of A. niger and competitive bioaccumulation under the coexistence of Pb and Cd, which would facilitate the application of microorganisms to the bioremediation of coexisted metals. |
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| language | English |
| last_indexed | 2024-04-10T23:32:20Z |
| publishDate | 2023-01-01 |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-f15ebd7d69eb47b7b3af0e25bcf7446b2023-01-12T05:46:54ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011010.3389/fbioe.2022.10963841096384Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stressShang Pan0Shang Pan1Shang Pan2Zhaoyan Li3Jiayi Wang4Xuefei Li5Lingzi Meng6Yunhui Chen7Mu Su8Zhen Li9Zhen Li10Zhen Li11College of Agro-grassland Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaJiangsu Key Laboratory for Organic Waste Utilization, Nanjing Agricultural University, Nanjing, ChinaCollege of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaJiangsu Key Laboratory for Organic Waste Utilization, Nanjing Agricultural University, Nanjing, ChinaState Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Nanjing, ChinaIn the bioremediation process, coexistence of lead (Pb) and cadmium causes complex toxicity, resulting in the difficulty of bioremediation. This study investigated the physiological responses and bioaccumulation mechanisms of the typical filamentous fungus Aspergillus niger under the coexistence of Pb and Cd. Four treatments were set up, i.e., control, sole Pb, sole Cd, and coexistence of Pb and Cd. The morphology of A. niger were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. Then, nano-scale secondary ion mass spectrometry (NanoSIMS) was applied to accurately investigate the distribution of heavy metals in the fungal cells under the coexistence of Pb and Cd. Finally, the metallogenic process and mineral types were simulated by Geochemist’s Workbench (GWB). The electron microscopic and NanoSIMS imaging showed that Pb and Cd were accumulated in both the extracellular and intracellular regions of the A. niger cells. In particular, the accumulated Pb content was ten times higher than that of Cd. However, Cd showed stronger toxicity than Pb to A. niger. Compared with the control treatment, Cd stress resulted in a two-fold increase of cell diameter and more extracellular substances, whereas the cell diameter increased nearly four times in the coexistence treatment. Moreover, the bioaccumulation of Pb was more intense than that of Cd during competitive sorption. The GWB simulation confirmed that Pb2+ can form multiple minerals (e.g., PbC2O4, PbHPO4, and Pb3(PO4)2, etc.), which significantly weakened its toxicity on the cell surface. This study elucidated the morphological characteristics of A. niger and competitive bioaccumulation under the coexistence of Pb and Cd, which would facilitate the application of microorganisms to the bioremediation of coexisted metals.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1096384/fullleadcadmiumAspergillus nigerelectron microscopyNanoSIMSGWB simulation |
| spellingShingle | Shang Pan Shang Pan Shang Pan Zhaoyan Li Jiayi Wang Xuefei Li Lingzi Meng Yunhui Chen Mu Su Zhen Li Zhen Li Zhen Li Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress Frontiers in Bioengineering and Biotechnology lead cadmium Aspergillus niger electron microscopy NanoSIMS GWB simulation |
| title | Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress |
| title_full | Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress |
| title_fullStr | Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress |
| title_full_unstemmed | Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress |
| title_short | Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress |
| title_sort | electron microscopic imaging and nanosims investigation on physiological responses of aspergillus niger under pb ii and cd ii stress |
| topic | lead cadmium Aspergillus niger electron microscopy NanoSIMS GWB simulation |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1096384/full |
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