Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagation
Successful micropropagation of black chokeberry (Aronia melanocarpa) cultivars ‘Nero’, ‘Melrom’ and ‘Galicjanka’ was realized using young shoots with the aim of an optimized hormones concentration for initiation, multiplication and rooting phases. Good potential of initiation was observed at ‘Nero’...
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| Format: | Article |
| Language: | English |
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Academy of Agricultural and Forestry Sciences
2022-12-01
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| Series: | Romanian Journal of Horticulture |
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| Online Access: | https://romanianjournalofhorticulture.ro/wp-content/uploads/2022/12/04.31-36.pdf |
| _version_ | 1797952958569119744 |
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| author | Zs. Jakab I L.D. Vlașin A.M. Chiorean |
| author_facet | Zs. Jakab I L.D. Vlașin A.M. Chiorean |
| author_sort | Zs. Jakab I |
| collection | DOAJ |
| description | Successful micropropagation of black chokeberry (Aronia melanocarpa) cultivars ‘Nero’, ‘Melrom’ and ‘Galicjanka’ was realized using young shoots with the aim of an optimized hormones concentration for initiation, multiplication and rooting phases. Good potential of initiation was observed at ‘Nero’ and ‘Galicjanka’ cultivars, respectively of shoots regeneration at ‘Galicjanka’ and ‘Melrom’ cultivars. Results
showed that the use of antioxidant solutions is crucially in the initiation phase in order to prevent explants oxidation. Good initiation rates were obtained at ‘Nero’ (57 %) and ‘Galicjanka’ (57%) cultivars, while for ‘Melrom’ the rate was only 33% by using a hormonal balance of 0.7 mg/L BAP and a reduced level of 0.3 mg/L GA3. The in vitro shoots multiplication rate was relatively stable during the first subcultures. Best micropropagation rates were obtained at ‘Galicjanka’ and ‘Melrom’ cultivars followed by ‘Nero’ cultivar, using a 0.7 mg/L BAP and 0.5 mg/L GA3. The study showed also that, rooting process is enhanced when the culture medium contains activated charcoal (1.2 g/L) and a lower IBA concentration (0.5 mg/L) compared with the application of higher auxin concentration of the same hormone (1.0 mg/L). Activated charcoal seems to promote a good rooting process, thus being very important in the rooting phase. Researches revealed that the genotype influences the capacity of inoculums regeneration, the multiplication capacity and the rooting ability. |
| first_indexed | 2024-04-10T22:54:14Z |
| format | Article |
| id | doaj.art-f1611ee635c64e38b20960ccf8f13a30 |
| institution | Directory Open Access Journal |
| issn | 2734-7656 2734-8083 |
| language | English |
| last_indexed | 2024-04-10T22:54:14Z |
| publishDate | 2022-12-01 |
| publisher | Academy of Agricultural and Forestry Sciences |
| record_format | Article |
| series | Romanian Journal of Horticulture |
| spelling | doaj.art-f1611ee635c64e38b20960ccf8f13a302023-01-14T19:27:00ZengAcademy of Agricultural and Forestry SciencesRomanian Journal of Horticulture2734-76562734-80832022-12-0133313610.51258/RJH.2022.04Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagationZs. Jakab I0L.D. Vlașin1A.M. Chiorean2Fruit Research and Development Station Bistrita, RomaniaFruit Research and Development Station Bistrita, Romania Fruit Research and Development Station Bistrita, RomaniaSuccessful micropropagation of black chokeberry (Aronia melanocarpa) cultivars ‘Nero’, ‘Melrom’ and ‘Galicjanka’ was realized using young shoots with the aim of an optimized hormones concentration for initiation, multiplication and rooting phases. Good potential of initiation was observed at ‘Nero’ and ‘Galicjanka’ cultivars, respectively of shoots regeneration at ‘Galicjanka’ and ‘Melrom’ cultivars. Results showed that the use of antioxidant solutions is crucially in the initiation phase in order to prevent explants oxidation. Good initiation rates were obtained at ‘Nero’ (57 %) and ‘Galicjanka’ (57%) cultivars, while for ‘Melrom’ the rate was only 33% by using a hormonal balance of 0.7 mg/L BAP and a reduced level of 0.3 mg/L GA3. The in vitro shoots multiplication rate was relatively stable during the first subcultures. Best micropropagation rates were obtained at ‘Galicjanka’ and ‘Melrom’ cultivars followed by ‘Nero’ cultivar, using a 0.7 mg/L BAP and 0.5 mg/L GA3. The study showed also that, rooting process is enhanced when the culture medium contains activated charcoal (1.2 g/L) and a lower IBA concentration (0.5 mg/L) compared with the application of higher auxin concentration of the same hormone (1.0 mg/L). Activated charcoal seems to promote a good rooting process, thus being very important in the rooting phase. Researches revealed that the genotype influences the capacity of inoculums regeneration, the multiplication capacity and the rooting ability. https://romanianjournalofhorticulture.ro/wp-content/uploads/2022/12/04.31-36.pdfpropagationrootingwoody lant medium‘nero’‘melrom’‘galicjanka’ |
| spellingShingle | Zs. Jakab I L.D. Vlașin A.M. Chiorean Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagation Romanian Journal of Horticulture propagation rooting woody lant medium ‘nero’ ‘melrom’ ‘galicjanka’ |
| title | Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagation |
| title_full | Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagation |
| title_fullStr | Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagation |
| title_full_unstemmed | Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagation |
| title_short | Behaviour of several chokeberry cultivars (Aronia melanocarpa) at the in vitro micropropagation |
| title_sort | behaviour of several chokeberry cultivars aronia melanocarpa at the in vitro micropropagation |
| topic | propagation rooting woody lant medium ‘nero’ ‘melrom’ ‘galicjanka’ |
| url | https://romanianjournalofhorticulture.ro/wp-content/uploads/2022/12/04.31-36.pdf |
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