Phytochemicals, Antioxidant Attributes and Larvicidal Activity of <em>Mercurialis annua</em> L. (<em>Euphorbiaceae</em>) Leaf Extracts against <em>Tribolium confusum</em> (Du Val) Larvae (<em>Coleoptera</em>; <em>Tenebrionidae</em>)
This study reports the link between larvicidal activity and the phytochemical composition of male and female leaf extracts of <i>Mercurialis annua</i> L. (<i>M</i>. <i>annua</i>) from four Tunisian regions: Bizerte, Jandouba, Nabeul and Beja. Their antioxidant act...
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/4/344 |
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| author | Rania Ben Nasr Elie Djantou Baudelaire Amadou Dicko Hela El Ferchichi Ouarda |
| author_facet | Rania Ben Nasr Elie Djantou Baudelaire Amadou Dicko Hela El Ferchichi Ouarda |
| author_sort | Rania Ben Nasr |
| collection | DOAJ |
| description | This study reports the link between larvicidal activity and the phytochemical composition of male and female leaf extracts of <i>Mercurialis annua</i> L. (<i>M</i>. <i>annua</i>) from four Tunisian regions: Bizerte, Jandouba, Nabeul and Beja. Their antioxidant activity was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assays. Phenolic compounds were identified and quantified using liquid chromatography coupled with a UV detector and mass spectrometry (LC-UV-ESI/MS). Higher antioxidant activity (AOA) was found in the leaves of male plant extracts than of female ones. The leaves of male and female plant extracts from Bizerte exhibited the highest AOA: 22.04 and 22.78 mg Trolox equivalent/g dry matter (mg TE/g DM), respectively. For both sexes, plant extracts from Beja had the lowest AOA with 19.71 and 19.67 mg TE/g DM for male and female plants, respectively. Some phenolic compounds such as narcissin, gallocatechin, rutin, epigallocatechin and epicatechin were identified and quantified using LC-MS, which highlighted the abundance of narcissin and rutin in the male leaves of <i>M</i>. <i>annua</i>. We noted that the interaction between the sex of plants and the provenance had a significant effect on TFC (F = 6.63; <i>p</i> = 0.004) and AOA (F = 6.53; <i>p</i> = 0.004) assays, but there was no interaction between sex and origins for TPC (F = 1.76; <i>p</i> = 0.19). The larvicidal activity of aqueous leaf extracts of <i>M</i>. <i>annua</i> against <i>Tribolium confusum</i> (Du Val) (<i>T</i>. <i>confusum</i>), an insect pest of flour and cereal seeds, showed that the mortality could reach 100% after 48 h of exposure in the Bizerte region. The LC<sub>50</sub> values for the leaf extract were low in Bizerte, with 0.003 and 0.009 g/mL for male and female plants, respectively, succeeded by Jandouba, which displayed 0.006 and 0.024 g/mL for male and female plants, respectively. Nabeul showed 0.025 g/mL for male plants and 0.046 g/mL for female plants and Beja showed 0.037 and 0.072 g/mL for male and female plants, respectively. This is the first time that a study has revealed a negative correlation between the antioxidant activity and the larvicidal activity of the leaf extracts of <i>M</i>. <i>annua</i> with the following correlation coefficients of Perason: r = −0.975 and r = −0.760 for male and female plants, respectively. |
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| spelling | doaj.art-f161c886923844b0a0b48f512098dcb12023-11-21T16:15:32ZengMDPI AGBiology2079-77372021-04-0110434410.3390/biology10040344Phytochemicals, Antioxidant Attributes and Larvicidal Activity of <em>Mercurialis annua</em> L. (<em>Euphorbiaceae</em>) Leaf Extracts against <em>Tribolium confusum</em> (Du Val) Larvae (<em>Coleoptera</em>; <em>Tenebrionidae</em>)Rania Ben Nasr0Elie Djantou Baudelaire1Amadou Dicko2Hela El Ferchichi Ouarda3Laboratoire de Chimie et de Physique—Approches Multi-échelles des Milieux Complexes (LCP-A2MC)1, Boulevard Arago, CEDEX 03, 57078 Metz, FranceAGRITECH, 4, Rue Piroux, 54000 Nancy, FranceLaboratoire de Chimie et de Physique—Approches Multi-échelles des Milieux Complexes (LCP-A2MC)1, Boulevard Arago, CEDEX 03, 57078 Metz, FranceLaboratory of Plant Toxicology & Environmental Microbiology, Faculty of Science of Bizerte, University of Carthage, Zarzouna 7021, TunisiaThis study reports the link between larvicidal activity and the phytochemical composition of male and female leaf extracts of <i>Mercurialis annua</i> L. (<i>M</i>. <i>annua</i>) from four Tunisian regions: Bizerte, Jandouba, Nabeul and Beja. Their antioxidant activity was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assays. Phenolic compounds were identified and quantified using liquid chromatography coupled with a UV detector and mass spectrometry (LC-UV-ESI/MS). Higher antioxidant activity (AOA) was found in the leaves of male plant extracts than of female ones. The leaves of male and female plant extracts from Bizerte exhibited the highest AOA: 22.04 and 22.78 mg Trolox equivalent/g dry matter (mg TE/g DM), respectively. For both sexes, plant extracts from Beja had the lowest AOA with 19.71 and 19.67 mg TE/g DM for male and female plants, respectively. Some phenolic compounds such as narcissin, gallocatechin, rutin, epigallocatechin and epicatechin were identified and quantified using LC-MS, which highlighted the abundance of narcissin and rutin in the male leaves of <i>M</i>. <i>annua</i>. We noted that the interaction between the sex of plants and the provenance had a significant effect on TFC (F = 6.63; <i>p</i> = 0.004) and AOA (F = 6.53; <i>p</i> = 0.004) assays, but there was no interaction between sex and origins for TPC (F = 1.76; <i>p</i> = 0.19). The larvicidal activity of aqueous leaf extracts of <i>M</i>. <i>annua</i> against <i>Tribolium confusum</i> (Du Val) (<i>T</i>. <i>confusum</i>), an insect pest of flour and cereal seeds, showed that the mortality could reach 100% after 48 h of exposure in the Bizerte region. The LC<sub>50</sub> values for the leaf extract were low in Bizerte, with 0.003 and 0.009 g/mL for male and female plants, respectively, succeeded by Jandouba, which displayed 0.006 and 0.024 g/mL for male and female plants, respectively. Nabeul showed 0.025 g/mL for male plants and 0.046 g/mL for female plants and Beja showed 0.037 and 0.072 g/mL for male and female plants, respectively. This is the first time that a study has revealed a negative correlation between the antioxidant activity and the larvicidal activity of the leaf extracts of <i>M</i>. <i>annua</i> with the following correlation coefficients of Perason: r = −0.975 and r = −0.760 for male and female plants, respectively.https://www.mdpi.com/2079-7737/10/4/344<i>Mercurialis annua</i> L.antioxidant activityLC-MSlarvicidal activity |
| spellingShingle | Rania Ben Nasr Elie Djantou Baudelaire Amadou Dicko Hela El Ferchichi Ouarda Phytochemicals, Antioxidant Attributes and Larvicidal Activity of <em>Mercurialis annua</em> L. (<em>Euphorbiaceae</em>) Leaf Extracts against <em>Tribolium confusum</em> (Du Val) Larvae (<em>Coleoptera</em>; <em>Tenebrionidae</em>) Biology <i>Mercurialis annua</i> L. antioxidant activity LC-MS larvicidal activity |
| title | Phytochemicals, Antioxidant Attributes and Larvicidal Activity of <em>Mercurialis annua</em> L. (<em>Euphorbiaceae</em>) Leaf Extracts against <em>Tribolium confusum</em> (Du Val) Larvae (<em>Coleoptera</em>; <em>Tenebrionidae</em>) |
| title_full | Phytochemicals, Antioxidant Attributes and Larvicidal Activity of <em>Mercurialis annua</em> L. (<em>Euphorbiaceae</em>) Leaf Extracts against <em>Tribolium confusum</em> (Du Val) Larvae (<em>Coleoptera</em>; <em>Tenebrionidae</em>) |
| title_fullStr | Phytochemicals, Antioxidant Attributes and Larvicidal Activity of <em>Mercurialis annua</em> L. (<em>Euphorbiaceae</em>) Leaf Extracts against <em>Tribolium confusum</em> (Du Val) Larvae (<em>Coleoptera</em>; <em>Tenebrionidae</em>) |
| title_full_unstemmed | Phytochemicals, Antioxidant Attributes and Larvicidal Activity of <em>Mercurialis annua</em> L. (<em>Euphorbiaceae</em>) Leaf Extracts against <em>Tribolium confusum</em> (Du Val) Larvae (<em>Coleoptera</em>; <em>Tenebrionidae</em>) |
| title_short | Phytochemicals, Antioxidant Attributes and Larvicidal Activity of <em>Mercurialis annua</em> L. (<em>Euphorbiaceae</em>) Leaf Extracts against <em>Tribolium confusum</em> (Du Val) Larvae (<em>Coleoptera</em>; <em>Tenebrionidae</em>) |
| title_sort | phytochemicals antioxidant attributes and larvicidal activity of em mercurialis annua em l em euphorbiaceae em leaf extracts against em tribolium confusum em du val larvae em coleoptera em em tenebrionidae em |
| topic | <i>Mercurialis annua</i> L. antioxidant activity LC-MS larvicidal activity |
| url | https://www.mdpi.com/2079-7737/10/4/344 |
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