Locus 9p21.3 – Genetic Predictor of Coronary Atherosclerosis Severity in Siberians
The purpose of this study was to investigate association between 9p21.3 locus single nucleotide polymorphisms (SNP) and severity of coronary atheromatous burden. A total of 255 Caucasians (211 male, 44 female) with myocardial infarction younger 65 years old (mean 52,56±7,98 years) were enrolled. Gen...
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| Format: | Article |
| Language: | English |
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Siberian Federal University
2013-06-01
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| Series: | Журнал Сибирского федерального университета: Серия Биология |
| Subjects: | |
| Online Access: | http://elib.sfu-kras.ru/bitstream/2311/9982/1/Shesternya.pdf |
| _version_ | 1818507404910788608 |
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| author | Pavel A. Shesternya Anna S. Sergeeva Svetlana Yu. Nikulina Vladimir A. Shulman |
| author_facet | Pavel A. Shesternya Anna S. Sergeeva Svetlana Yu. Nikulina Vladimir A. Shulman |
| author_sort | Pavel A. Shesternya |
| collection | DOAJ |
| description | The purpose of this study was to investigate association between 9p21.3 locus single nucleotide polymorphisms (SNP) and severity of coronary atheromatous burden. A total of 255 Caucasians (211 male, 44 female) with myocardial infarction younger 65 years old (mean 52,56±7,98 years) were enrolled. Genome DNA was extracted from venous blood using the phenol-chloroform extraction method. Two SNPs rs10757278 and rs1333049 (locus 9p21.3) were tested using real-time polymerase chain reaction (PCR) according to protocol (probes TaqMan, Applied Biosystems, 7900HT). The coronary angiograms were reviewed by independent angiographers who were blinded to the results of the genotype (Philips Allura Xper FD10). The total number of lesions, Gensini scores and SYNTAX score were derived. For the first time in males of Siberian population, genotype CC rs1333049 and genotype GG rs10757278 demonstrated a direct strong association with severity of coronary atheromatous burden |
| first_indexed | 2024-12-10T22:17:58Z |
| format | Article |
| id | doaj.art-f16631949f804c4cb31e810247a37c50 |
| institution | Directory Open Access Journal |
| issn | 1997-1389 2313-5530 |
| language | English |
| last_indexed | 2024-12-10T22:17:58Z |
| publishDate | 2013-06-01 |
| publisher | Siberian Federal University |
| record_format | Article |
| series | Журнал Сибирского федерального университета: Серия Биология |
| spelling | doaj.art-f16631949f804c4cb31e810247a37c502022-12-22T01:31:25ZengSiberian Federal UniversityЖурнал Сибирского федерального университета: Серия Биология1997-13892313-55302013-06-0162175184http://elib.sfu-kras.ru/handle/2311/9982Locus 9p21.3 – Genetic Predictor of Coronary Atherosclerosis Severity in SiberiansPavel A. Shesternya0Anna S. Sergeeva1Svetlana Yu. Nikulina2Vladimir A. Shulman3Krasnoyarsk State Medical University named after Рrof. V.F. Voino-Yasenetsky, 1 Partizan Zheleznyak Str., Krasnoyarsk, 660022 RussiaKrasnoyarsk State Medical University named after Рrof. V.F. Voino-Yasenetsky, 1 Partizan Zheleznyak Str., Krasnoyarsk, 660022 RussiaKrasnoyarsk State Medical University named after Рrof. V.F. Voino-Yasenetsky, 1 Partizan Zheleznyak Str., Krasnoyarsk, 660022 RussiaKrasnoyarsk State Medical University named after Рrof. V.F. Voino-Yasenetsky, 1 Partizan Zheleznyak Str., Krasnoyarsk, 660022 RussiaThe purpose of this study was to investigate association between 9p21.3 locus single nucleotide polymorphisms (SNP) and severity of coronary atheromatous burden. A total of 255 Caucasians (211 male, 44 female) with myocardial infarction younger 65 years old (mean 52,56±7,98 years) were enrolled. Genome DNA was extracted from venous blood using the phenol-chloroform extraction method. Two SNPs rs10757278 and rs1333049 (locus 9p21.3) were tested using real-time polymerase chain reaction (PCR) according to protocol (probes TaqMan, Applied Biosystems, 7900HT). The coronary angiograms were reviewed by independent angiographers who were blinded to the results of the genotype (Philips Allura Xper FD10). The total number of lesions, Gensini scores and SYNTAX score were derived. For the first time in males of Siberian population, genotype CC rs1333049 and genotype GG rs10757278 demonstrated a direct strong association with severity of coronary atheromatous burdenhttp://elib.sfu-kras.ru/bitstream/2311/9982/1/Shesternya.pdfrs10757278rs1333049myocardial infarctionsingle nucleotide polymorphismsrs10757278rs1333049locus 9p21.3atherosclerosis |
| spellingShingle | Pavel A. Shesternya Anna S. Sergeeva Svetlana Yu. Nikulina Vladimir A. Shulman Locus 9p21.3 – Genetic Predictor of Coronary Atherosclerosis Severity in Siberians Журнал Сибирского федерального университета: Серия Биология rs10757278 rs1333049 myocardial infarction single nucleotide polymorphisms rs10757278 rs1333049 locus 9p21.3 atherosclerosis |
| title | Locus 9p21.3 – Genetic Predictor of Coronary Atherosclerosis Severity in Siberians |
| title_full | Locus 9p21.3 – Genetic Predictor of Coronary Atherosclerosis Severity in Siberians |
| title_fullStr | Locus 9p21.3 – Genetic Predictor of Coronary Atherosclerosis Severity in Siberians |
| title_full_unstemmed | Locus 9p21.3 – Genetic Predictor of Coronary Atherosclerosis Severity in Siberians |
| title_short | Locus 9p21.3 – Genetic Predictor of Coronary Atherosclerosis Severity in Siberians |
| title_sort | locus 9p21 3 genetic predictor of coronary atherosclerosis severity in siberians |
| topic | rs10757278 rs1333049 myocardial infarction single nucleotide polymorphisms rs10757278 rs1333049 locus 9p21.3 atherosclerosis |
| url | http://elib.sfu-kras.ru/bitstream/2311/9982/1/Shesternya.pdf |
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