Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis
IntroductionUntil now, the mechanism underlying the impact of topping on hormone regulation in tobacco plants remains unclear, and most studies investigating the hormone signaling pathways in plants rely on genes or transcriptional pathways.MethodsThis study examines the regulatory mechanisms of hor...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1255252/full |
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| author | Kaiyuan Gu Li-E. Yang Ke Ren Xianxue Luo Xiao Qin Michiel Op de Beeck Conglian He Li Jian Yi Chen |
| author_facet | Kaiyuan Gu Li-E. Yang Ke Ren Xianxue Luo Xiao Qin Michiel Op de Beeck Conglian He Li Jian Yi Chen |
| author_sort | Kaiyuan Gu |
| collection | DOAJ |
| description | IntroductionUntil now, the mechanism underlying the impact of topping on hormone regulation in tobacco plants remains unclear, and most studies investigating the hormone signaling pathways in plants rely on genes or transcriptional pathways.MethodsThis study examines the regulatory mechanisms of hormones in the roots and leaves of tobacco plants with and without topping at the protein level.ResultsThe results demonstrate that, compared with non-topped plants, topping leads to a decrease in the levels of IAA (auxin), ABA (abscisic acid), and GA (gibberellin) hormones in the leaves, whereas the content of the JA (jasmonic acid) hormone increases. Furthermore, in the roots, topping results in an increase in the levels of IAA, ABA, and JA hormones, along with a decrease in GA content. In the leaves, a total of 258 significantly different proteins were identified before and after topping, with 128 proteins upregulated and 130 proteins downregulated. In the roots, there were 439 proteins with significantly different quantities before and after topping, consisting of 211 upregulated proteins and 228 downregulated proteins. Notably, these proteins were closely associated with the metabolic and biosynthetic pathways of secondary metabolites, as indicated by functional categorization.ConclusionsWhen integrating the hormone changes and the proteomics results, it is evident that topping leads to increased metabolic activity and enhanced hormone synthesis in the root system. This research provides a theoretical foundation for further investigations into the regulation and signaling mechanisms of hormones at the protein level before and after topping in plants. |
| first_indexed | 2024-03-11T14:44:51Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T14:44:51Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-f16738304741461abb6e1f6e2cecad772023-10-30T12:09:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-10-011410.3389/fpls.2023.12552521255252Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysisKaiyuan Gu0Li-E. Yang1Ke Ren2Xianxue Luo3Xiao Qin4Michiel Op de Beeck5Conglian He6Li Jian7Yi Chen8Agronomic Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, ChinaBiotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, ChinaAgronomic Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, ChinaRaw material center, Hunan Zhangjiajie Municipal Tobacco Co., Zhangjiajie, Hunan, ChinaRaw material center, Hunan Zhangjiajie Municipal Tobacco Co., Zhangjiajie, Hunan, ChinaCentre for Environmental and Climate Research, Lund University, Lund, SwedenAgronomic Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, ChinaKey Laboratory of Urban Environment and Health, Instituteo of Urban Enviroment, Chinense Academy of Sciences, Xiamen, Fujian, ChinaAgronomic Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, ChinaIntroductionUntil now, the mechanism underlying the impact of topping on hormone regulation in tobacco plants remains unclear, and most studies investigating the hormone signaling pathways in plants rely on genes or transcriptional pathways.MethodsThis study examines the regulatory mechanisms of hormones in the roots and leaves of tobacco plants with and without topping at the protein level.ResultsThe results demonstrate that, compared with non-topped plants, topping leads to a decrease in the levels of IAA (auxin), ABA (abscisic acid), and GA (gibberellin) hormones in the leaves, whereas the content of the JA (jasmonic acid) hormone increases. Furthermore, in the roots, topping results in an increase in the levels of IAA, ABA, and JA hormones, along with a decrease in GA content. In the leaves, a total of 258 significantly different proteins were identified before and after topping, with 128 proteins upregulated and 130 proteins downregulated. In the roots, there were 439 proteins with significantly different quantities before and after topping, consisting of 211 upregulated proteins and 228 downregulated proteins. Notably, these proteins were closely associated with the metabolic and biosynthetic pathways of secondary metabolites, as indicated by functional categorization.ConclusionsWhen integrating the hormone changes and the proteomics results, it is evident that topping leads to increased metabolic activity and enhanced hormone synthesis in the root system. This research provides a theoretical foundation for further investigations into the regulation and signaling mechanisms of hormones at the protein level before and after topping in plants.https://www.frontiersin.org/articles/10.3389/fpls.2023.1255252/fulltobaccoproteomicsleaves and rootsplant hormonestopping |
| spellingShingle | Kaiyuan Gu Li-E. Yang Ke Ren Xianxue Luo Xiao Qin Michiel Op de Beeck Conglian He Li Jian Yi Chen Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis Frontiers in Plant Science tobacco proteomics leaves and roots plant hormones topping |
| title | Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis |
| title_full | Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis |
| title_fullStr | Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis |
| title_full_unstemmed | Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis |
| title_short | Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis |
| title_sort | effects of topping and non topping on growth regulating hormones of flue cured tobacco nicotiana tabacum l a proteomic analysis |
| topic | tobacco proteomics leaves and roots plant hormones topping |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1255252/full |
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