Morpho-molecular, cultural and pathological characterization of Athelia rolfsii causing southern blight disease on common bean
Common bean (Phaseolus vulgaris), is a winter legume crop in Bangladesh and is considered an important vegetable with export potential. However, the production of common bean is severely affected by a newly reported soilborne fungal pathogen, Athelia rolfsii. This study aimed to characterize this ne...
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Elsevier
2023-05-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023033431 |
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| author | Swapan Kumar Paul Dipali Rani Gupta Chandan Kumar Mahapatra Kanistha Rani Tofazzal Islam |
| author_facet | Swapan Kumar Paul Dipali Rani Gupta Chandan Kumar Mahapatra Kanistha Rani Tofazzal Islam |
| author_sort | Swapan Kumar Paul |
| collection | DOAJ |
| description | Common bean (Phaseolus vulgaris), is a winter legume crop in Bangladesh and is considered an important vegetable with export potential. However, the production of common bean is severely affected by a newly reported soilborne fungal pathogen, Athelia rolfsii. This study aimed to characterize this new pathogen by morphological, molecular, cultural, and pathological analyses and determine the host range. The disease incidence in the affected field ranged between 6 and 13%. Initial disease symptoms were observed as brown sunken lesions at the point of infection and development of mycelia, followed by yellowing and quick wilting of the whole plant. A total of 10 fungal isolates were recovered from the infected plant samples, which were morphologically similar and produced white to brown mycelia and numerous brown sclerotia on the PDA medium. Two of them viz. BTCBSr3 and BTCBSr4 were used for the detailed study. Based on morphology and phylogenetic analyses of the sequenced data of internal transcribed spacer (ITS) and translation elongation factor 1 alpha (EF-1α), the pathogen was identified as A. rolfsii. Mycelial growth rate (3.6 cm/day) and fresh weight (107 mg) were higher in the PDA medium, whereas the number of sclerotia production (328/plate) was higher in OMA media. The isolates could grow in a wider range of incubation temperatures (15–35 °C) and media pH (3–9). In the cross-inoculation assay, both isolates were pathogenic on tomato, brinjal, and chickpea, but not on chili, soybean, and cowpea. This study has laid a foundation for further pathological research on the fungus in aid to develop an effective management practice against the pathogen. |
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| format | Article |
| id | doaj.art-f16de10ad6534f23b0d4cdad7ea27756 |
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| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:25:22Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
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| spelling | doaj.art-f16de10ad6534f23b0d4cdad7ea277562023-05-31T04:46:43ZengElsevierHeliyon2405-84402023-05-0195e16136Morpho-molecular, cultural and pathological characterization of Athelia rolfsii causing southern blight disease on common beanSwapan Kumar Paul0Dipali Rani Gupta1Chandan Kumar Mahapatra2Kanistha Rani3Tofazzal Islam4Department of Agronomy, Bangladesh Agricultural University, Mymensingh, 2202, BangladeshInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, BangladeshDepartment of Agronomy, Bangladesh Agricultural University, Mymensingh, 2202, BangladeshInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, BangladeshInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh; Corresponding author.Common bean (Phaseolus vulgaris), is a winter legume crop in Bangladesh and is considered an important vegetable with export potential. However, the production of common bean is severely affected by a newly reported soilborne fungal pathogen, Athelia rolfsii. This study aimed to characterize this new pathogen by morphological, molecular, cultural, and pathological analyses and determine the host range. The disease incidence in the affected field ranged between 6 and 13%. Initial disease symptoms were observed as brown sunken lesions at the point of infection and development of mycelia, followed by yellowing and quick wilting of the whole plant. A total of 10 fungal isolates were recovered from the infected plant samples, which were morphologically similar and produced white to brown mycelia and numerous brown sclerotia on the PDA medium. Two of them viz. BTCBSr3 and BTCBSr4 were used for the detailed study. Based on morphology and phylogenetic analyses of the sequenced data of internal transcribed spacer (ITS) and translation elongation factor 1 alpha (EF-1α), the pathogen was identified as A. rolfsii. Mycelial growth rate (3.6 cm/day) and fresh weight (107 mg) were higher in the PDA medium, whereas the number of sclerotia production (328/plate) was higher in OMA media. The isolates could grow in a wider range of incubation temperatures (15–35 °C) and media pH (3–9). In the cross-inoculation assay, both isolates were pathogenic on tomato, brinjal, and chickpea, but not on chili, soybean, and cowpea. This study has laid a foundation for further pathological research on the fungus in aid to develop an effective management practice against the pathogen.http://www.sciencedirect.com/science/article/pii/S2405844023033431Fungal morphologyITSPhylogenyPathogenicityHost range |
| spellingShingle | Swapan Kumar Paul Dipali Rani Gupta Chandan Kumar Mahapatra Kanistha Rani Tofazzal Islam Morpho-molecular, cultural and pathological characterization of Athelia rolfsii causing southern blight disease on common bean Heliyon Fungal morphology ITS Phylogeny Pathogenicity Host range |
| title | Morpho-molecular, cultural and pathological characterization of Athelia rolfsii causing southern blight disease on common bean |
| title_full | Morpho-molecular, cultural and pathological characterization of Athelia rolfsii causing southern blight disease on common bean |
| title_fullStr | Morpho-molecular, cultural and pathological characterization of Athelia rolfsii causing southern blight disease on common bean |
| title_full_unstemmed | Morpho-molecular, cultural and pathological characterization of Athelia rolfsii causing southern blight disease on common bean |
| title_short | Morpho-molecular, cultural and pathological characterization of Athelia rolfsii causing southern blight disease on common bean |
| title_sort | morpho molecular cultural and pathological characterization of athelia rolfsii causing southern blight disease on common bean |
| topic | Fungal morphology ITS Phylogeny Pathogenicity Host range |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023033431 |
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