Application of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifolii
The paper evaluates the competitive ability of Rhizobium leguminosarum bv trifolii strain T49B1 used as inoculant for Trifolium repens L. (Viola) and indigenous population of rhizobia from chernozem, stagnosol and humic gleysol. Nodule bacteria were identified using calcofluor effects (CF) based on...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Institute of field and vegetable crops, Novi Sad
2012-01-01
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| Series: | Ratarstvo i Povrtarstvo |
| Subjects: | |
| Online Access: | http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2012/1821-39441202167J.pdf |
| _version_ | 1818289553563189248 |
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| author | Jošić Dragana Rasulić Nataša Kuzmanović Đorđe Miladinović Miroslav Stanojković Aleksandra Pivić Radmila |
| author_facet | Jošić Dragana Rasulić Nataša Kuzmanović Đorđe Miladinović Miroslav Stanojković Aleksandra Pivić Radmila |
| author_sort | Jošić Dragana |
| collection | DOAJ |
| description | The paper evaluates the competitive ability of Rhizobium leguminosarum bv trifolii strain T49B1 used as inoculant for Trifolium repens L. (Viola) and indigenous population of rhizobia from chernozem, stagnosol and humic gleysol. Nodule bacteria were identified using calcofluor effects (CF) based on succinoglycan production, and confirmed by IAR patterns and RAPD fingerprinting (using SPH1 primer). T49B1 nodules occupancy level from 71.78% in pseudoglay to 89.56% in chernozem was detected. Inoculation by T49B1 strain showed it to be a very good competitor with the possibility to establish effective symbiosis and increase protein content compared to non-inoculated (control) plants. |
| first_indexed | 2024-12-13T02:14:07Z |
| format | Article |
| id | doaj.art-f17851ce6a4d426b901cb15e13c93ba2 |
| institution | Directory Open Access Journal |
| issn | 1821-3944 2217-8392 |
| language | English |
| last_indexed | 2024-12-13T02:14:07Z |
| publishDate | 2012-01-01 |
| publisher | Institute of field and vegetable crops, Novi Sad |
| record_format | Article |
| series | Ratarstvo i Povrtarstvo |
| spelling | doaj.art-f17851ce6a4d426b901cb15e13c93ba22022-12-22T00:02:57ZengInstitute of field and vegetable crops, Novi SadRatarstvo i Povrtarstvo1821-39442217-83922012-01-0149216717210.5937/ratpov49-15861821-39441202167JApplication of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifoliiJošić Dragana0Rasulić Nataša1Kuzmanović Đorđe2Miladinović Miroslav3Stanojković Aleksandra4Pivić Radmila5Institute of Soil Sciences, Belgrade, SerbiaInstitute of Soil Sciences, Belgrade, SerbiaInstitute of Soil Sciences, Belgrade, SerbiaInstitute of Soil Sciences, Belgrade, SerbiaInstitute of Soil Sciences, Belgrade, SerbiaInstitute of Soil Sciences, Belgrade, SerbiaThe paper evaluates the competitive ability of Rhizobium leguminosarum bv trifolii strain T49B1 used as inoculant for Trifolium repens L. (Viola) and indigenous population of rhizobia from chernozem, stagnosol and humic gleysol. Nodule bacteria were identified using calcofluor effects (CF) based on succinoglycan production, and confirmed by IAR patterns and RAPD fingerprinting (using SPH1 primer). T49B1 nodules occupancy level from 71.78% in pseudoglay to 89.56% in chernozem was detected. Inoculation by T49B1 strain showed it to be a very good competitor with the possibility to establish effective symbiosis and increase protein content compared to non-inoculated (control) plants.http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2012/1821-39441202167J.pdfcalcofluor effectIARRAPDRhizobiumsoilsstrains |
| spellingShingle | Jošić Dragana Rasulić Nataša Kuzmanović Đorđe Miladinović Miroslav Stanojković Aleksandra Pivić Radmila Application of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifolii Ratarstvo i Povrtarstvo calcofluor effect IAR RAPD Rhizobium soils strains |
| title | Application of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifolii |
| title_full | Application of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifolii |
| title_fullStr | Application of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifolii |
| title_full_unstemmed | Application of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifolii |
| title_short | Application of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifolii |
| title_sort | application of cf effect iar and rapd methods in evaluation or competitiveness between inoculated and indigenous strains of rhizobium leguminosarum bv trifolii |
| topic | calcofluor effect IAR RAPD Rhizobium soils strains |
| url | http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2012/1821-39441202167J.pdf |
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