Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia
<p>Abstract</p> <p>Background</p> <p>The neuroprotective effect of xenon has been demonstrated for glutamatergic neurons. In the present study it is investigated if dopaminergic neurons, i.e. nerve-growth-factor differentiated PC-12 cells, are protected as well against...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2004-12-01
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| Series: | BMC Neuroscience |
| Online Access: | http://www.biomedcentral.com/1471-2202/5/55 |
| _version_ | 1818565614610939904 |
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| author | Schmehl Wolfgang Müller Jana Blom Per Petzelt Christian Kox Wolfgang J |
| author_facet | Schmehl Wolfgang Müller Jana Blom Per Petzelt Christian Kox Wolfgang J |
| author_sort | Schmehl Wolfgang |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The neuroprotective effect of xenon has been demonstrated for glutamatergic neurons. In the present study it is investigated if dopaminergic neurons, i.e. nerve-growth-factor differentiated PC-12 cells, are protected as well against hypoxia-induced cell damage in the presence of xenon.</p> <p>Results</p> <p>Pheochromocytoma cells differentiated by addition of nerve growth factor were placed in a N<sub>2</sub>-saturated atmosphere, a treatment that induced release of dopamine, reaching a maximum after 30 min. By determining extracellular lactate dehydrogenase concentration as marker for concomitant cellular damage, a substantial increase of enzymatic activity was found for N<sub>2</sub>-treated cells. Replacement of N<sub>2 </sub>by xenon in such a hypoxic atmosphere resulted in complete protection against cellular damage and prevention of hypoxia-induced dopamine release. Intracellular buffering of Ca<sup>2+ </sup>using the Ca-chelator 1, 2-<it>bis</it>(2-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid <it>tetrakis</it>(acetoxymethyl) ester (BAPTA) reduced the neuroprotective effect of xenon indicating the essential participation of intracellular Ca<sup>2+</sup>-ions in the process of xenon-induced neuroprotection.</p> <p>Conclusions</p> <p>The results presented demonstrate the outstanding property of xenon to protect neuron-like cells in a hypoxic situation.</p> |
| first_indexed | 2024-12-14T01:43:32Z |
| format | Article |
| id | doaj.art-f179db86ffe943ac942b645052d66b2b |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-12-14T01:43:32Z |
| publishDate | 2004-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-f179db86ffe943ac942b645052d66b2b2022-12-21T23:21:38ZengBMCBMC Neuroscience1471-22022004-12-01515510.1186/1471-2202-5-55Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxiaSchmehl WolfgangMüller JanaBlom PerPetzelt ChristianKox Wolfgang J<p>Abstract</p> <p>Background</p> <p>The neuroprotective effect of xenon has been demonstrated for glutamatergic neurons. In the present study it is investigated if dopaminergic neurons, i.e. nerve-growth-factor differentiated PC-12 cells, are protected as well against hypoxia-induced cell damage in the presence of xenon.</p> <p>Results</p> <p>Pheochromocytoma cells differentiated by addition of nerve growth factor were placed in a N<sub>2</sub>-saturated atmosphere, a treatment that induced release of dopamine, reaching a maximum after 30 min. By determining extracellular lactate dehydrogenase concentration as marker for concomitant cellular damage, a substantial increase of enzymatic activity was found for N<sub>2</sub>-treated cells. Replacement of N<sub>2 </sub>by xenon in such a hypoxic atmosphere resulted in complete protection against cellular damage and prevention of hypoxia-induced dopamine release. Intracellular buffering of Ca<sup>2+ </sup>using the Ca-chelator 1, 2-<it>bis</it>(2-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid <it>tetrakis</it>(acetoxymethyl) ester (BAPTA) reduced the neuroprotective effect of xenon indicating the essential participation of intracellular Ca<sup>2+</sup>-ions in the process of xenon-induced neuroprotection.</p> <p>Conclusions</p> <p>The results presented demonstrate the outstanding property of xenon to protect neuron-like cells in a hypoxic situation.</p>http://www.biomedcentral.com/1471-2202/5/55 |
| spellingShingle | Schmehl Wolfgang Müller Jana Blom Per Petzelt Christian Kox Wolfgang J Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia BMC Neuroscience |
| title | Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia |
| title_full | Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia |
| title_fullStr | Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia |
| title_full_unstemmed | Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia |
| title_short | Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia |
| title_sort | xenon prevents cellular damage in differentiated pc 12 cells exposed to hypoxia |
| url | http://www.biomedcentral.com/1471-2202/5/55 |
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