Tumor-Targeted Polydopamine-Based Nanoparticles for Multimodal Mapping Following Photothermal Therapy of Metastatic Lymph Nodes
Yanrui Liang,1,* Weihong Guo,1,* Chuangji Li,1,* Guodong Shen,1,* Haoxian Tan,2 Peiwen Sun,2 Zhian Chen,1 Huilin Huang,1 Zhenhao Li,1 Zhenyuan Li,1 Yingxin Ren,1 Guoxin Li,1 Yanfeng Hu1 1Department of General Surgery & Guangdong Provincial Key Laboratory of Precis...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2022-09-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/tumor-targeted-polydopamine-based-nanoparticles-for-multimodal-mapping-peer-reviewed-fulltext-article-IJN |
| _version_ | 1811203181904920576 |
|---|---|
| author | Liang Y Guo W Li C Shen G Tan H Sun P Chen Z Huang H Li Z Li Z Ren Y Li G Hu Y |
| author_facet | Liang Y Guo W Li C Shen G Tan H Sun P Chen Z Huang H Li Z Li Z Ren Y Li G Hu Y |
| author_sort | Liang Y |
| collection | DOAJ |
| description | Yanrui Liang,1,* Weihong Guo,1,* Chuangji Li,1,* Guodong Shen,1,* Haoxian Tan,2 Peiwen Sun,2 Zhian Chen,1 Huilin Huang,1 Zhenhao Li,1 Zhenyuan Li,1 Yingxin Ren,1 Guoxin Li,1 Yanfeng Hu1 1Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2The First School of Clinical Medicine, Southern Medical University, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guoxin Li; Yanfeng Hu, Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, Southern Medical University, No. 1838 North Guangzhou Ave, Baiyun District, Guangzhou, 510515, People’s Republic of China, Tel +86-20-6164-1681 ; +86-20-6164-1682, Fax +86-20-6164-1681, Email yfenghu@qq.com; gzliguoxin@163.comPurpose: Lymphadenectomy with lymph node (LN) mapping is essential for surgical removal of solid tumors. Existing agents do not provide accurate multimodal mapping and antitumor therapy for metastatic LNs; therefore, we fabricated a polydopamine (PDA) nanoparticle (NP)-based tumor-targeted LN mapping agent capable of multimodal mapping and guided photothermal therapy (PTT) for metastatic LNs.Materials and Methods: PDA NPs modified with polyethylene glycol (PEG) were obtained by polymerization under alkaline conditions. The PEG-PDA NPs were loaded with the circular tripeptide Arg-Gly-Asp (cRGD) to achieve tumor-targeting capacity and with the fluorescent dye IR820 and magnetic resonance imaging (MRI) contrast Gd(NH2)2 for in situ detection. The resulting cRGD-PEG-PDA@IR820/Gd(NH2)2 (cRGD-PPIG) NPs were tested for their biosafety and metastatic LN mapping ability. They were drained specifically into LNs and selectively taken up by gastric MKN45 cells via αvβ 3 integrin-mediated endocytosis.Results: This phenomenon enabled MR/optical/near-infrared fluorescence multimodal metastatic LN mapping, guiding the creation of accurate and highly efficient PTT for gastric cancer metastatic LNs in mice.Conclusion: In summary, we fabricated tumor-targeted cRGD-PPIG NPs with MR/optical/near-infrared fluorescence multimodal metastatic LN mapping capacity for surgery and efficient PTT guidance post-surgery.Keywords: multimodal imaging, lymph node mapping, metastatic lymph node, photothermal therapy |
| first_indexed | 2024-04-12T02:51:21Z |
| format | Article |
| id | doaj.art-f17c263476b740c399f7dd3b0792ccc5 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-04-12T02:51:21Z |
| publishDate | 2022-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-f17c263476b740c399f7dd3b0792ccc52022-12-22T03:50:59ZengDove Medical PressInternational Journal of Nanomedicine1178-20132022-09-01Volume 174659467578630Tumor-Targeted Polydopamine-Based Nanoparticles for Multimodal Mapping Following Photothermal Therapy of Metastatic Lymph NodesLiang YGuo WLi CShen GTan HSun PChen ZHuang HLi ZLi ZRen YLi GHu YYanrui Liang,1,* Weihong Guo,1,* Chuangji Li,1,* Guodong Shen,1,* Haoxian Tan,2 Peiwen Sun,2 Zhian Chen,1 Huilin Huang,1 Zhenhao Li,1 Zhenyuan Li,1 Yingxin Ren,1 Guoxin Li,1 Yanfeng Hu1 1Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2The First School of Clinical Medicine, Southern Medical University, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guoxin Li; Yanfeng Hu, Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, Southern Medical University, No. 1838 North Guangzhou Ave, Baiyun District, Guangzhou, 510515, People’s Republic of China, Tel +86-20-6164-1681 ; +86-20-6164-1682, Fax +86-20-6164-1681, Email yfenghu@qq.com; gzliguoxin@163.comPurpose: Lymphadenectomy with lymph node (LN) mapping is essential for surgical removal of solid tumors. Existing agents do not provide accurate multimodal mapping and antitumor therapy for metastatic LNs; therefore, we fabricated a polydopamine (PDA) nanoparticle (NP)-based tumor-targeted LN mapping agent capable of multimodal mapping and guided photothermal therapy (PTT) for metastatic LNs.Materials and Methods: PDA NPs modified with polyethylene glycol (PEG) were obtained by polymerization under alkaline conditions. The PEG-PDA NPs were loaded with the circular tripeptide Arg-Gly-Asp (cRGD) to achieve tumor-targeting capacity and with the fluorescent dye IR820 and magnetic resonance imaging (MRI) contrast Gd(NH2)2 for in situ detection. The resulting cRGD-PEG-PDA@IR820/Gd(NH2)2 (cRGD-PPIG) NPs were tested for their biosafety and metastatic LN mapping ability. They were drained specifically into LNs and selectively taken up by gastric MKN45 cells via αvβ 3 integrin-mediated endocytosis.Results: This phenomenon enabled MR/optical/near-infrared fluorescence multimodal metastatic LN mapping, guiding the creation of accurate and highly efficient PTT for gastric cancer metastatic LNs in mice.Conclusion: In summary, we fabricated tumor-targeted cRGD-PPIG NPs with MR/optical/near-infrared fluorescence multimodal metastatic LN mapping capacity for surgery and efficient PTT guidance post-surgery.Keywords: multimodal imaging, lymph node mapping, metastatic lymph node, photothermal therapyhttps://www.dovepress.com/tumor-targeted-polydopamine-based-nanoparticles-for-multimodal-mapping-peer-reviewed-fulltext-article-IJNmultimodal imaginglymph node mappingmetastatic lymph nodephotothermal therapy |
| spellingShingle | Liang Y Guo W Li C Shen G Tan H Sun P Chen Z Huang H Li Z Li Z Ren Y Li G Hu Y Tumor-Targeted Polydopamine-Based Nanoparticles for Multimodal Mapping Following Photothermal Therapy of Metastatic Lymph Nodes International Journal of Nanomedicine multimodal imaging lymph node mapping metastatic lymph node photothermal therapy |
| title | Tumor-Targeted Polydopamine-Based Nanoparticles for Multimodal Mapping Following Photothermal Therapy of Metastatic Lymph Nodes |
| title_full | Tumor-Targeted Polydopamine-Based Nanoparticles for Multimodal Mapping Following Photothermal Therapy of Metastatic Lymph Nodes |
| title_fullStr | Tumor-Targeted Polydopamine-Based Nanoparticles for Multimodal Mapping Following Photothermal Therapy of Metastatic Lymph Nodes |
| title_full_unstemmed | Tumor-Targeted Polydopamine-Based Nanoparticles for Multimodal Mapping Following Photothermal Therapy of Metastatic Lymph Nodes |
| title_short | Tumor-Targeted Polydopamine-Based Nanoparticles for Multimodal Mapping Following Photothermal Therapy of Metastatic Lymph Nodes |
| title_sort | tumor targeted polydopamine based nanoparticles for multimodal mapping following photothermal therapy of metastatic lymph nodes |
| topic | multimodal imaging lymph node mapping metastatic lymph node photothermal therapy |
| url | https://www.dovepress.com/tumor-targeted-polydopamine-based-nanoparticles-for-multimodal-mapping-peer-reviewed-fulltext-article-IJN |
| work_keys_str_mv | AT liangy tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT guow tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT lic tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT sheng tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT tanh tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT sunp tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT chenz tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT huangh tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT liz tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT liz tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT reny tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT lig tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes AT huy tumortargetedpolydopaminebasednanoparticlesformultimodalmappingfollowingphotothermaltherapyofmetastaticlymphnodes |