Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein
Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.668870/full |
| _version_ | 1818669437247553536 |
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| author | Amna Didar Abbasi Zakir Hussain Usman Liaqat Dooa Arif Kun-Lin Yang |
| author_facet | Amna Didar Abbasi Zakir Hussain Usman Liaqat Dooa Arif Kun-Lin Yang |
| author_sort | Amna Didar Abbasi |
| collection | DOAJ |
| description | Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon a hydrophobic glass slide with surface immobilized gp-120. When the glass surface is incubated in a solution containing B40t77, the aptamer is able to bind to gp-120 specifically and remove it from the surface after a short incubation time of 30 min. The result of the binding event can be amplified by using liquid crystal (LC) into optical signals in the final step. By using this bioassay, we are able to detect as low as 1 μg/ml of gp-120 with high specificity within 30 min. No response is obtained when gp-120 is replaced by other protein such as bovine serum albumin (BSA). This is the first qualitative bioassay which provides a simple way for the detection of gp-120 with the naked eye. The assay is robust, low-cost and does not require additional labeling. Thus, the bioassay is potentially useful for the early detection of HIV-1 in resources-limited regions. |
| first_indexed | 2024-12-17T06:52:12Z |
| format | Article |
| id | doaj.art-f17cf3fb0e87497d920db9d61cc88af1 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-17T06:52:12Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-f17cf3fb0e87497d920db9d61cc88af12022-12-21T21:59:34ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-04-01910.3389/fchem.2021.668870668870Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface GlycoproteinAmna Didar Abbasi0Zakir Hussain1Usman Liaqat2Dooa Arif3Kun-Lin Yang4Department of Materials Engineering, School of Chemical and Materials Engineering, National University of Sciences and Technology, Islamabad, PakistanDepartment of Materials Engineering, School of Chemical and Materials Engineering, National University of Sciences and Technology, Islamabad, PakistanDepartment of Materials Engineering, School of Chemical and Materials Engineering, National University of Sciences and Technology, Islamabad, PakistanDepartment of Materials Engineering, School of Chemical and Materials Engineering, National University of Sciences and Technology, Islamabad, PakistanDepartment of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, SingaporeSurface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon a hydrophobic glass slide with surface immobilized gp-120. When the glass surface is incubated in a solution containing B40t77, the aptamer is able to bind to gp-120 specifically and remove it from the surface after a short incubation time of 30 min. The result of the binding event can be amplified by using liquid crystal (LC) into optical signals in the final step. By using this bioassay, we are able to detect as low as 1 μg/ml of gp-120 with high specificity within 30 min. No response is obtained when gp-120 is replaced by other protein such as bovine serum albumin (BSA). This is the first qualitative bioassay which provides a simple way for the detection of gp-120 with the naked eye. The assay is robust, low-cost and does not require additional labeling. Thus, the bioassay is potentially useful for the early detection of HIV-1 in resources-limited regions.https://www.frontiersin.org/articles/10.3389/fchem.2021.668870/fullliquid crystalsaptamergp-120binding assayPDMS |
| spellingShingle | Amna Didar Abbasi Zakir Hussain Usman Liaqat Dooa Arif Kun-Lin Yang Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein Frontiers in Chemistry liquid crystals aptamer gp-120 binding assay PDMS |
| title | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
| title_full | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
| title_fullStr | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
| title_full_unstemmed | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
| title_short | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
| title_sort | liquid crystal based binding assay for detecting hiv 1 surface glycoprotein |
| topic | liquid crystals aptamer gp-120 binding assay PDMS |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.668870/full |
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