Two Oncoviruses of HPV and EBV in Breast Cancer: An Iran-based Study
OBJECTIVE: To answer the question of whether viral infections such as Human papillomavirus (HPV) and Epstein-Barr virus (EBV) are involved in the occurrence of breast cancer or not. METHODOLOGY: In this case-control study, 50 paraffin-embedded breast samples (FFPE) and 50 non-cancerous FFPE sample...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Liaquat University of Medical and Health Sciences
2023-09-01
|
| Series: | JLUMHS |
| Subjects: |
| _version_ | 1797658942415831040 |
|---|---|
| author | Mona Fani Riam Sadooni Mahmoud Haghighat Nejad Neda Naseri Mirzavieh Sara Mobarak Reza Pakzad Yousef Erfani Shokrollah Salmanzadeh Samaneh Abbasi |
| author_facet | Mona Fani Riam Sadooni Mahmoud Haghighat Nejad Neda Naseri Mirzavieh Sara Mobarak Reza Pakzad Yousef Erfani Shokrollah Salmanzadeh Samaneh Abbasi |
| author_sort | Mona Fani |
| collection | DOAJ |
| description | OBJECTIVE: To answer the question of whether viral infections such as Human papillomavirus (HPV) and Epstein-Barr virus (EBV) are involved in the occurrence of breast cancer or not.
METHODOLOGY: In this case-control study, 50 paraffin-embedded breast samples (FFPE) and 50 non-cancerous FFPE samples were prepared from patients referred to Abadan hospitals from 2018 to 2021 to identify the genome of HPV and EBV by polymerase chain reaction (PCR). Data were analyzed using SPSS 15 with P-values of 0.05. Confirmed breast cancer biopsy samples with no medical history of chemotherapy and/or radiotherapy, the patient not being pregnant at the time of sampling, not using anti-cancer treatments, and not suffering from systemic inflammatory diseases such as rheumatoid arthritis. Patients of any age, with any breast cancer, and any size or stage of tumor were included without restrictions.
RESULTS: Using PCR, HPV DNA was detected in 7/50 (14%) and 2/50(4%) of breast cancers and normal control, respectively. Moreover, 71.42% and 28.57% of HPV-infected persons in the patient's group were genotypes 16 and 18, respectively. All HPV-infected people in the control group were low-risk genotypes 26 and 53. Also, EBV DNA was found in 2/50 (4%) and 6/50 (12%) of breast cancers and control samples, respectively. Co-infection was not detected in samples.
CONCLUSION: Although our study does not provide substantial Evidence about the role of viral infection in the progressiveness of breast tumors, significant studies have shown the critical role of these infections in the progression of breast tumors. |
| first_indexed | 2024-03-11T18:06:43Z |
| format | Article |
| id | doaj.art-f17eaa24e8a24322a16faf04f077ba13 |
| institution | Directory Open Access Journal |
| issn | 1729-0341 2309-8627 |
| language | English |
| last_indexed | 2024-03-11T18:06:43Z |
| publishDate | 2023-09-01 |
| publisher | Liaquat University of Medical and Health Sciences |
| record_format | Article |
| series | JLUMHS |
| spelling | doaj.art-f17eaa24e8a24322a16faf04f077ba132023-10-17T06:50:03ZengLiaquat University of Medical and Health SciencesJLUMHS1729-03412309-86272023-09-0122316917310.22442/jlumhs.2023.01033Two Oncoviruses of HPV and EBV in Breast Cancer: An Iran-based StudyMona Fani0Riam Sadooni1Mahmoud Haghighat Nejad2Neda Naseri Mirzavieh3Sara Mobarak4Reza Pakzad5Yousef Erfani6Shokrollah Salmanzadeh7 Samaneh Abbasi8Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.Abadan University of Medical Sciences, Abadan, Iran.Anatomical and Clinical Pathology Laboratory, Dr Haghighat Nejad, MD, Abadan, Iran.Tehran Shargh Pyame Noor University (PNU), Tehran, Iran.Abadan University of Medical Sciences, Abadan, Iran.Department of Epidemiology, Faculty of Health, Ilam University Medical Sciences, Ilam, Iran.Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University Medical Sciences, Tehran, Iran. Infectious and Tropical Diseases Research Center, Health Research Institute, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran.OBJECTIVE: To answer the question of whether viral infections such as Human papillomavirus (HPV) and Epstein-Barr virus (EBV) are involved in the occurrence of breast cancer or not. METHODOLOGY: In this case-control study, 50 paraffin-embedded breast samples (FFPE) and 50 non-cancerous FFPE samples were prepared from patients referred to Abadan hospitals from 2018 to 2021 to identify the genome of HPV and EBV by polymerase chain reaction (PCR). Data were analyzed using SPSS 15 with P-values of 0.05. Confirmed breast cancer biopsy samples with no medical history of chemotherapy and/or radiotherapy, the patient not being pregnant at the time of sampling, not using anti-cancer treatments, and not suffering from systemic inflammatory diseases such as rheumatoid arthritis. Patients of any age, with any breast cancer, and any size or stage of tumor were included without restrictions. RESULTS: Using PCR, HPV DNA was detected in 7/50 (14%) and 2/50(4%) of breast cancers and normal control, respectively. Moreover, 71.42% and 28.57% of HPV-infected persons in the patient's group were genotypes 16 and 18, respectively. All HPV-infected people in the control group were low-risk genotypes 26 and 53. Also, EBV DNA was found in 2/50 (4%) and 6/50 (12%) of breast cancers and control samples, respectively. Co-infection was not detected in samples. CONCLUSION: Although our study does not provide substantial Evidence about the role of viral infection in the progressiveness of breast tumors, significant studies have shown the critical role of these infections in the progression of breast tumors.human papillomavirushpvepstein–barr virusebvbreast cancer. |
| spellingShingle | Mona Fani Riam Sadooni Mahmoud Haghighat Nejad Neda Naseri Mirzavieh Sara Mobarak Reza Pakzad Yousef Erfani Shokrollah Salmanzadeh Samaneh Abbasi Two Oncoviruses of HPV and EBV in Breast Cancer: An Iran-based Study JLUMHS human papillomavirus hpv epstein–barr virus ebv breast cancer. |
| title | Two Oncoviruses of HPV and EBV in Breast Cancer: An Iran-based Study |
| title_full | Two Oncoviruses of HPV and EBV in Breast Cancer: An Iran-based Study |
| title_fullStr | Two Oncoviruses of HPV and EBV in Breast Cancer: An Iran-based Study |
| title_full_unstemmed | Two Oncoviruses of HPV and EBV in Breast Cancer: An Iran-based Study |
| title_short | Two Oncoviruses of HPV and EBV in Breast Cancer: An Iran-based Study |
| title_sort | two oncoviruses of hpv and ebv in breast cancer an iran based study |
| topic | human papillomavirus hpv epstein–barr virus ebv breast cancer. |
| work_keys_str_mv | AT monafani twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy AT riamsadooni twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy AT mahmoudhaghighatnejad twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy AT nedanaserimirzavieh twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy AT saramobarak twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy AT rezapakzad twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy AT youseferfani twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy AT shokrollahsalmanzadeh twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy AT samanehabbasi twooncovirusesofhpvandebvinbreastcanceraniranbasedstudy |