Venomics of the ectoparasitoid wasp Bracon nigricans
Abstract Background Venom is one of the most important sources of regulation factors used by parasitic Hymenoptera to redirect host physiology in favour of the developing offspring. This has stimulated a number of studies, both at functional and “omics” level, which, however, are still quite limited...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-01-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-019-6396-4 |
| _version_ | 1819279088091660288 |
|---|---|
| author | Andrea Becchimanzi Maddalena Avolio Hamed Bostan Chiara Colantuono Flora Cozzolino Donato Mancini Maria Luisa Chiusano Pietro Pucci Silvia Caccia Francesco Pennacchio |
| author_facet | Andrea Becchimanzi Maddalena Avolio Hamed Bostan Chiara Colantuono Flora Cozzolino Donato Mancini Maria Luisa Chiusano Pietro Pucci Silvia Caccia Francesco Pennacchio |
| author_sort | Andrea Becchimanzi |
| collection | DOAJ |
| description | Abstract Background Venom is one of the most important sources of regulation factors used by parasitic Hymenoptera to redirect host physiology in favour of the developing offspring. This has stimulated a number of studies, both at functional and “omics” level, which, however, are still quite limited for ectophagous parasitoids that permanently paralyze and suppress their victims (i.e., idiobiont parasitoids). Results Here we present a combined transcriptomic and proteomic study of the venom of the generalist idiobiont wasp Bracon nigricans, an ectophagous larval parasitoid of different lepidopteran species, for which we recently described the host regulation strategy and the functional role of the venom in the induction of physiological changes in parasitized hosts. The experimental approach used led to the identification of the main components of B. nigricans venom involved in host regulation. Enzymes degrading lipids, proteins and carbohydrates are likely involved in the mobilization of storage nutrients from the fat body and may concurrently be responsible for the release of neurotoxic fatty acids inducing paralysis, and for the modulation of host immune responses. Conclusion The present work contributes to fill the gap of knowledge on venom composition in ectoparasitoid wasps, and, along with our previous physiological study on this species, provides the foundation on which to develop a functional model of host regulation, based both on physiological and molecular data. This paves the way towards a better understanding of parasitism evolution in the basal lineages of Hymenoptera and to the possible exploitation of venom as source of bioinsecticidal molecules. |
| first_indexed | 2024-12-24T00:22:20Z |
| format | Article |
| id | doaj.art-f18202731a16417abd764ff5cd044936 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-24T00:22:20Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-f18202731a16417abd764ff5cd0449362022-12-21T17:24:33ZengBMCBMC Genomics1471-21642020-01-0121111510.1186/s12864-019-6396-4Venomics of the ectoparasitoid wasp Bracon nigricansAndrea Becchimanzi0Maddalena Avolio1Hamed Bostan2Chiara Colantuono3Flora Cozzolino4Donato Mancini5Maria Luisa Chiusano6Pietro Pucci7Silvia Caccia8Francesco Pennacchio9Department of Agricultural Sciences, University of Napoli Federico IIDepartment of Agricultural Sciences, University of Napoli Federico IIDepartment of Agricultural Sciences, University of Napoli Federico IIDepartment of Agricultural Sciences, University of Napoli Federico IIDepartment of Chemical Sciences and CEINGE Biotecnologie Avanzate, University of Napoli Federico IIDepartment of Agricultural Sciences, University of Napoli Federico IIDepartment of Agricultural Sciences, University of Napoli Federico IIDepartment of Chemical Sciences and CEINGE Biotecnologie Avanzate, University of Napoli Federico IIDepartment of Agricultural Sciences, University of Napoli Federico IIDepartment of Agricultural Sciences, University of Napoli Federico IIAbstract Background Venom is one of the most important sources of regulation factors used by parasitic Hymenoptera to redirect host physiology in favour of the developing offspring. This has stimulated a number of studies, both at functional and “omics” level, which, however, are still quite limited for ectophagous parasitoids that permanently paralyze and suppress their victims (i.e., idiobiont parasitoids). Results Here we present a combined transcriptomic and proteomic study of the venom of the generalist idiobiont wasp Bracon nigricans, an ectophagous larval parasitoid of different lepidopteran species, for which we recently described the host regulation strategy and the functional role of the venom in the induction of physiological changes in parasitized hosts. The experimental approach used led to the identification of the main components of B. nigricans venom involved in host regulation. Enzymes degrading lipids, proteins and carbohydrates are likely involved in the mobilization of storage nutrients from the fat body and may concurrently be responsible for the release of neurotoxic fatty acids inducing paralysis, and for the modulation of host immune responses. Conclusion The present work contributes to fill the gap of knowledge on venom composition in ectoparasitoid wasps, and, along with our previous physiological study on this species, provides the foundation on which to develop a functional model of host regulation, based both on physiological and molecular data. This paves the way towards a better understanding of parasitism evolution in the basal lineages of Hymenoptera and to the possible exploitation of venom as source of bioinsecticidal molecules.https://doi.org/10.1186/s12864-019-6396-4Idiobiont parasitoidsHost-parasitoid interactionsVenom proteomeVenom transcriptomeHost regulation |
| spellingShingle | Andrea Becchimanzi Maddalena Avolio Hamed Bostan Chiara Colantuono Flora Cozzolino Donato Mancini Maria Luisa Chiusano Pietro Pucci Silvia Caccia Francesco Pennacchio Venomics of the ectoparasitoid wasp Bracon nigricans BMC Genomics Idiobiont parasitoids Host-parasitoid interactions Venom proteome Venom transcriptome Host regulation |
| title | Venomics of the ectoparasitoid wasp Bracon nigricans |
| title_full | Venomics of the ectoparasitoid wasp Bracon nigricans |
| title_fullStr | Venomics of the ectoparasitoid wasp Bracon nigricans |
| title_full_unstemmed | Venomics of the ectoparasitoid wasp Bracon nigricans |
| title_short | Venomics of the ectoparasitoid wasp Bracon nigricans |
| title_sort | venomics of the ectoparasitoid wasp bracon nigricans |
| topic | Idiobiont parasitoids Host-parasitoid interactions Venom proteome Venom transcriptome Host regulation |
| url | https://doi.org/10.1186/s12864-019-6396-4 |
| work_keys_str_mv | AT andreabecchimanzi venomicsoftheectoparasitoidwaspbraconnigricans AT maddalenaavolio venomicsoftheectoparasitoidwaspbraconnigricans AT hamedbostan venomicsoftheectoparasitoidwaspbraconnigricans AT chiaracolantuono venomicsoftheectoparasitoidwaspbraconnigricans AT floracozzolino venomicsoftheectoparasitoidwaspbraconnigricans AT donatomancini venomicsoftheectoparasitoidwaspbraconnigricans AT marialuisachiusano venomicsoftheectoparasitoidwaspbraconnigricans AT pietropucci venomicsoftheectoparasitoidwaspbraconnigricans AT silviacaccia venomicsoftheectoparasitoidwaspbraconnigricans AT francescopennacchio venomicsoftheectoparasitoidwaspbraconnigricans |