Production of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141
Monoclonal antibodies (MAb) were produced against two B. pseudomallei high-purified membrane proteins with Mr 29 kDa (p29) and 45 kDa (p45). Monoclonal antibodies from culture supernatant fluids of 4F2 and 1G11 clones showed specific interaction with protein moiety of p29 both Burkholderia pseudomal...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2008-08-01
|
| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1021 |
| _version_ | 1797221045400240128 |
|---|---|
| author | S. S. Vetchinin P. C. Kopylov N. V. Kiseleva A. M. Baranov E. V. Baranova E. V. Galkina A. I. Borzilov I. Y. Kalachev |
| author_facet | S. S. Vetchinin P. C. Kopylov N. V. Kiseleva A. M. Baranov E. V. Baranova E. V. Galkina A. I. Borzilov I. Y. Kalachev |
| author_sort | S. S. Vetchinin |
| collection | DOAJ |
| description | Monoclonal antibodies (MAb) were produced against two B. pseudomallei high-purified membrane proteins with Mr 29 kDa (p29) and 45 kDa (p45). Monoclonal antibodies from culture supernatant fluids of 4F2 and 1G11 clones showed specific interaction with protein moiety of p29 both Burkholderia pseudomallei and Burkholderia mallei in ELISA and Western blotting. However, MAb of 3G4 clone were bound to the LPS-protein structures of these microbial cells. Analysis of interaction of Mabs from 4F2 and 1G11 clones with antigens of different lysates of pathogenic cells confirmed high specificity of these antibodies to p29 membrane protein of B. pseudomallei and B. mallei. |
| first_indexed | 2024-03-08T13:44:56Z |
| format | Article |
| id | doaj.art-f18237c85fe54afd8f6430b837c03b8f |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:59:11Z |
| publishDate | 2008-08-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-f18237c85fe54afd8f6430b837c03b8f2024-04-05T16:47:05ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2008-08-0104(98)545710.21055/0370-1069-2008-4(98)-54-571004Production of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141S. S. Vetchinin0P. C. Kopylov1N. V. Kiseleva2A. M. Baranov3E. V. Baranova4E. V. Galkina5A. I. Borzilov6I. Y. Kalachev7State Research Center for Applied Microbiology and Biotechnology, ObolenskState Research Center for Applied Microbiology and Biotechnology, ObolenskState Research Center for Applied Microbiology and Biotechnology, ObolenskState Research Center for Applied Microbiology and Biotechnology, ObolenskState Research Center for Applied Microbiology and Biotechnology, ObolenskState Research Center for Applied Microbiology and Biotechnology, ObolenskState Research Center for Applied Microbiology and Biotechnology, ObolenskBranch o f Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow RegionMonoclonal antibodies (MAb) were produced against two B. pseudomallei high-purified membrane proteins with Mr 29 kDa (p29) and 45 kDa (p45). Monoclonal antibodies from culture supernatant fluids of 4F2 and 1G11 clones showed specific interaction with protein moiety of p29 both Burkholderia pseudomallei and Burkholderia mallei in ELISA and Western blotting. However, MAb of 3G4 clone were bound to the LPS-protein structures of these microbial cells. Analysis of interaction of Mabs from 4F2 and 1G11 clones with antigens of different lysates of pathogenic cells confirmed high specificity of these antibodies to p29 membrane protein of B. pseudomallei and B. mallei.https://journal.microbe.ru/jour/article/view/1021burkholderia pseudomalleiburkholderia malleiouther membrane proteinsmonoclonal antibodies |
| spellingShingle | S. S. Vetchinin P. C. Kopylov N. V. Kiseleva A. M. Baranov E. V. Baranova E. V. Galkina A. I. Borzilov I. Y. Kalachev Production of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141 Проблемы особо опасных инфекций burkholderia pseudomallei burkholderia mallei outher membrane proteins monoclonal antibodies |
| title | Production of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141 |
| title_full | Production of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141 |
| title_fullStr | Production of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141 |
| title_full_unstemmed | Production of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141 |
| title_short | Production of Monoclonal Antibodies against Outer Membrane Proteins of <i>Burkholderia pseudomallei</i>, Strain C-141 |
| title_sort | production of monoclonal antibodies against outer membrane proteins of i burkholderia pseudomallei i strain c 141 |
| topic | burkholderia pseudomallei burkholderia mallei outher membrane proteins monoclonal antibodies |
| url | https://journal.microbe.ru/jour/article/view/1021 |
| work_keys_str_mv | AT ssvetchinin productionofmonoclonalantibodiesagainstoutermembraneproteinsofiburkholderiapseudomalleiistrainc141 AT pckopylov productionofmonoclonalantibodiesagainstoutermembraneproteinsofiburkholderiapseudomalleiistrainc141 AT nvkiseleva productionofmonoclonalantibodiesagainstoutermembraneproteinsofiburkholderiapseudomalleiistrainc141 AT ambaranov productionofmonoclonalantibodiesagainstoutermembraneproteinsofiburkholderiapseudomalleiistrainc141 AT evbaranova productionofmonoclonalantibodiesagainstoutermembraneproteinsofiburkholderiapseudomalleiistrainc141 AT evgalkina productionofmonoclonalantibodiesagainstoutermembraneproteinsofiburkholderiapseudomalleiistrainc141 AT aiborzilov productionofmonoclonalantibodiesagainstoutermembraneproteinsofiburkholderiapseudomalleiistrainc141 AT iykalachev productionofmonoclonalantibodiesagainstoutermembraneproteinsofiburkholderiapseudomalleiistrainc141 |