Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection
An impedimetric immunosensor for fumonisin B1 (FB1) was developed from a poly(2,5-dimethoxyaniline)-multi-walled carbon nanotube (PDMA-MWCNT) composite on the surface of glassy carbon electrode (GCE). The composite was prepared electrochemically and characterized using cyclic voltammetry. The prepar...
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2016-04-01
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| Online Access: | http://www.mdpi.com/1996-1944/9/4/273 |
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| author | Milua Masikini Avril R. Williams Christopher E. Sunday Tesfaye T. Waryo Ezo Nxusani Lindsay Wilson Sinazo Qakala Mawethu Bilibana Samantha Douman Anovuyo Jonnas Priscilla G. L. Baker Emmanuel I. Iwuoha |
| author_facet | Milua Masikini Avril R. Williams Christopher E. Sunday Tesfaye T. Waryo Ezo Nxusani Lindsay Wilson Sinazo Qakala Mawethu Bilibana Samantha Douman Anovuyo Jonnas Priscilla G. L. Baker Emmanuel I. Iwuoha |
| author_sort | Milua Masikini |
| collection | DOAJ |
| description | An impedimetric immunosensor for fumonisin B1 (FB1) was developed from a poly(2,5-dimethoxyaniline)-multi-walled carbon nanotube (PDMA-MWCNT) composite on the surface of glassy carbon electrode (GCE). The composite was prepared electrochemically and characterized using cyclic voltammetry. The preparation of the FB1 immunosensor involved the drop-coating of a bovine serum albumin mixture of the anti-fumonisin antibody (anti-Fms) onto the composite polymer-modified GCE. The electrochemical impedance spectroscopy (EIS) responses of the FB1 immunosensor (GCE/PDMA-MWCNT/anti-Fms) have a linear range of 7 to 49 ng·L−1, and the corresponding sensitivity and detection limits are 0.272 kΩ L·ng−1 and 3.8 pg·L−1, respectively. The limit of detection of the immunosensor for certified corn sample (i.e., certified reference material) is 0.014 ppm FB1, which is in excellent agreement with the value published by the vendors and significantly more accurate than that obtained with enzyme-linked immunosorbent assay (ELISA). |
| first_indexed | 2024-04-12T23:48:36Z |
| format | Article |
| id | doaj.art-f182b95e6d7b4bf2a61eecc66145d048 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-04-12T23:48:36Z |
| publishDate | 2016-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-f182b95e6d7b4bf2a61eecc66145d0482022-12-22T03:11:46ZengMDPI AGMaterials1996-19442016-04-019427310.3390/ma9040273ma9040273Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 DetectionMilua Masikini0Avril R. Williams1Christopher E. Sunday2Tesfaye T. Waryo3Ezo Nxusani4Lindsay Wilson5Sinazo Qakala6Mawethu Bilibana7Samantha Douman8Anovuyo Jonnas9Priscilla G. L. Baker10Emmanuel I. Iwuoha11SensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaDepartment of Biological and Chemical Sciences, The University of the West Indies, Cave Hill Campus, Bridgetown BB11000, BarbadosSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaSensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South AfricaAn impedimetric immunosensor for fumonisin B1 (FB1) was developed from a poly(2,5-dimethoxyaniline)-multi-walled carbon nanotube (PDMA-MWCNT) composite on the surface of glassy carbon electrode (GCE). The composite was prepared electrochemically and characterized using cyclic voltammetry. The preparation of the FB1 immunosensor involved the drop-coating of a bovine serum albumin mixture of the anti-fumonisin antibody (anti-Fms) onto the composite polymer-modified GCE. The electrochemical impedance spectroscopy (EIS) responses of the FB1 immunosensor (GCE/PDMA-MWCNT/anti-Fms) have a linear range of 7 to 49 ng·L−1, and the corresponding sensitivity and detection limits are 0.272 kΩ L·ng−1 and 3.8 pg·L−1, respectively. The limit of detection of the immunosensor for certified corn sample (i.e., certified reference material) is 0.014 ppm FB1, which is in excellent agreement with the value published by the vendors and significantly more accurate than that obtained with enzyme-linked immunosorbent assay (ELISA).http://www.mdpi.com/1996-1944/9/4/273fumonisin B1anti-fumonisin antibodyimmunosensorpoly(2,5-dimethoxyaniline)-multi-walled carbon nanotubescertified reference materialselectrochemical impedance spectroscopy |
| spellingShingle | Milua Masikini Avril R. Williams Christopher E. Sunday Tesfaye T. Waryo Ezo Nxusani Lindsay Wilson Sinazo Qakala Mawethu Bilibana Samantha Douman Anovuyo Jonnas Priscilla G. L. Baker Emmanuel I. Iwuoha Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection Materials fumonisin B1 anti-fumonisin antibody immunosensor poly(2,5-dimethoxyaniline)-multi-walled carbon nanotubes certified reference materials electrochemical impedance spectroscopy |
| title | Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection |
| title_full | Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection |
| title_fullStr | Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection |
| title_full_unstemmed | Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection |
| title_short | Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection |
| title_sort | label free poly 2 5 dimethoxyaniline multi walled carbon nanotubes impedimetric immunosensor for fumonisin b1 detection |
| topic | fumonisin B1 anti-fumonisin antibody immunosensor poly(2,5-dimethoxyaniline)-multi-walled carbon nanotubes certified reference materials electrochemical impedance spectroscopy |
| url | http://www.mdpi.com/1996-1944/9/4/273 |
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