Physico-Chemical Quality and Physiological Profiles of Microbial Communities in Freshwater Systems of Mega Manila, Philippines
Studying the quality of freshwater systems and drinking water in highly urbanized megalopolises around the world remains a challenge. This article reports data on the quality of select freshwater systems in Mega Manila, Philippines. Water samples collected between 2020 and 2021 were analyzed for phy...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Online Access: | https://www.mdpi.com/2306-5729/8/6/103 |
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| author | Marie Christine M. Obusan Arizaldo E. Castro Ren Mark D. Villanueva Margareth Del E. Isagan Jamaica Ann A. Caras Jessica F. Simbahan |
| author_facet | Marie Christine M. Obusan Arizaldo E. Castro Ren Mark D. Villanueva Margareth Del E. Isagan Jamaica Ann A. Caras Jessica F. Simbahan |
| author_sort | Marie Christine M. Obusan |
| collection | DOAJ |
| description | Studying the quality of freshwater systems and drinking water in highly urbanized megalopolises around the world remains a challenge. This article reports data on the quality of select freshwater systems in Mega Manila, Philippines. Water samples collected between 2020 and 2021 were analyzed for physico-chemical parameters and microbial community metabolic fingerprints, i.e., carbon substrate utilization patterns (CSUPs). The detection of arsenic, lead, cadmium, mercury, polyaromatic hydrocarbons (PAHs), and organochlorine pesticides (OCPs) was carried out using standard chromatography- and spectroscopy-based protocols. Physiological profiles were determined using the Biolog EcoPlate™ system. Eight samples were free of heavy metals, and none contained PAHs or OCPs. Fourteen samples had high microbial activity, as indicated by average well color development (AWCD) and community metabolic diversity (CMD) values. Community-level physiological profiling (CLPP) revealed that (1) samples clustered as groups according to shared CSUPs, and (2) microbial communities in non-drinking samples actively utilized all six substrate classes compared to drinking samples. The data reported here can provide a baseline or a comparator for prospective quality assessments of drinking water and freshwater sources in the region. Metabolic fingerprinting using CSUPs is a simple and cheap phenotypic analysis of microbial communities and their physiological activity in aquatic environments. |
| first_indexed | 2024-03-11T02:36:20Z |
| format | Article |
| id | doaj.art-f1841eb6b22542a39ccc8c644acfdc0e |
| institution | Directory Open Access Journal |
| issn | 2306-5729 |
| language | English |
| last_indexed | 2024-03-11T02:36:20Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Data |
| spelling | doaj.art-f1841eb6b22542a39ccc8c644acfdc0e2023-11-18T09:58:30ZengMDPI AGData2306-57292023-06-018610310.3390/data8060103Physico-Chemical Quality and Physiological Profiles of Microbial Communities in Freshwater Systems of Mega Manila, PhilippinesMarie Christine M. Obusan0Arizaldo E. Castro1Ren Mark D. Villanueva2Margareth Del E. Isagan3Jamaica Ann A. Caras4Jessica F. Simbahan5Microbial Ecology of Terrestrial and Aquatic Systems Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesMicrobial Ecology of Terrestrial and Aquatic Systems Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesMicrobial Ecology of Terrestrial and Aquatic Systems Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesMicrobial Ecology of Terrestrial and Aquatic Systems Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesMicrobial Ecology of Terrestrial and Aquatic Systems Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesMicrobial Ecology of Terrestrial and Aquatic Systems Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesStudying the quality of freshwater systems and drinking water in highly urbanized megalopolises around the world remains a challenge. This article reports data on the quality of select freshwater systems in Mega Manila, Philippines. Water samples collected between 2020 and 2021 were analyzed for physico-chemical parameters and microbial community metabolic fingerprints, i.e., carbon substrate utilization patterns (CSUPs). The detection of arsenic, lead, cadmium, mercury, polyaromatic hydrocarbons (PAHs), and organochlorine pesticides (OCPs) was carried out using standard chromatography- and spectroscopy-based protocols. Physiological profiles were determined using the Biolog EcoPlate™ system. Eight samples were free of heavy metals, and none contained PAHs or OCPs. Fourteen samples had high microbial activity, as indicated by average well color development (AWCD) and community metabolic diversity (CMD) values. Community-level physiological profiling (CLPP) revealed that (1) samples clustered as groups according to shared CSUPs, and (2) microbial communities in non-drinking samples actively utilized all six substrate classes compared to drinking samples. The data reported here can provide a baseline or a comparator for prospective quality assessments of drinking water and freshwater sources in the region. Metabolic fingerprinting using CSUPs is a simple and cheap phenotypic analysis of microbial communities and their physiological activity in aquatic environments.https://www.mdpi.com/2306-5729/8/6/103drinking waterheavy metalsemerging pollutantsmicrobial communitieswater quality |
| spellingShingle | Marie Christine M. Obusan Arizaldo E. Castro Ren Mark D. Villanueva Margareth Del E. Isagan Jamaica Ann A. Caras Jessica F. Simbahan Physico-Chemical Quality and Physiological Profiles of Microbial Communities in Freshwater Systems of Mega Manila, Philippines Data drinking water heavy metals emerging pollutants microbial communities water quality |
| title | Physico-Chemical Quality and Physiological Profiles of Microbial Communities in Freshwater Systems of Mega Manila, Philippines |
| title_full | Physico-Chemical Quality and Physiological Profiles of Microbial Communities in Freshwater Systems of Mega Manila, Philippines |
| title_fullStr | Physico-Chemical Quality and Physiological Profiles of Microbial Communities in Freshwater Systems of Mega Manila, Philippines |
| title_full_unstemmed | Physico-Chemical Quality and Physiological Profiles of Microbial Communities in Freshwater Systems of Mega Manila, Philippines |
| title_short | Physico-Chemical Quality and Physiological Profiles of Microbial Communities in Freshwater Systems of Mega Manila, Philippines |
| title_sort | physico chemical quality and physiological profiles of microbial communities in freshwater systems of mega manila philippines |
| topic | drinking water heavy metals emerging pollutants microbial communities water quality |
| url | https://www.mdpi.com/2306-5729/8/6/103 |
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