Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2018-06-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/33506 |
| _version_ | 1818019590864633856 |
|---|---|
| author | Eiichi Murakami Jeryl Cheng Kira Gysel Zoltan Bozsoki Yasuyuki Kawaharada Christian Toftegaard Hjuler Kasper Kildegaard Sørensen Ke Tao Simon Kelly Francesco Venice Andrea Genre Mikkel Boas Thygesen Noor de Jong Maria Vinther Dorthe Bødker Jensen Knud Jørgen Jensen Michael Blaise Lene Heegaard Madsen Kasper Røjkjær Andersen Jens Stougaard Simona Radutoiu |
| author_facet | Eiichi Murakami Jeryl Cheng Kira Gysel Zoltan Bozsoki Yasuyuki Kawaharada Christian Toftegaard Hjuler Kasper Kildegaard Sørensen Ke Tao Simon Kelly Francesco Venice Andrea Genre Mikkel Boas Thygesen Noor de Jong Maria Vinther Dorthe Bødker Jensen Knud Jørgen Jensen Michael Blaise Lene Heegaard Madsen Kasper Røjkjær Andersen Jens Stougaard Simona Radutoiu |
| author_sort | Eiichi Murakami |
| collection | DOAJ |
| description | Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia. |
| first_indexed | 2024-04-14T07:54:21Z |
| format | Article |
| id | doaj.art-f186ae85f7914adc864d38a10cf3ef4a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:54:21Z |
| publishDate | 2018-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-f186ae85f7914adc864d38a10cf3ef4a2022-12-22T02:05:07ZengeLife Sciences Publications LtdeLife2050-084X2018-06-01710.7554/eLife.33506Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicusEiichi Murakami0https://orcid.org/0000-0001-6414-0254Jeryl Cheng1Kira Gysel2Zoltan Bozsoki3https://orcid.org/0000-0002-4267-9969Yasuyuki Kawaharada4Christian Toftegaard Hjuler5https://orcid.org/0000-0001-6055-6391Kasper Kildegaard Sørensen6Ke Tao7Simon Kelly8Francesco Venice9Andrea Genre10https://orcid.org/0000-0001-5029-6194Mikkel Boas Thygesen11https://orcid.org/0000-0002-0158-2802Noor de Jong12Maria Vinther13Dorthe Bødker Jensen14Knud Jørgen Jensen15Michael Blaise16Lene Heegaard Madsen17Kasper Røjkjær Andersen18https://orcid.org/0000-0002-4415-8067Jens Stougaard19https://orcid.org/0000-0002-9312-2685Simona Radutoiu20https://orcid.org/0000-0002-8841-1415Department of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Chemistry, University of Copenhagen, Frederiksberg, DenmarkDepartment of Chemistry, University of Copenhagen, Frederiksberg, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Life Sciences and Systems Biology, University of Torino, Torino, ItalyDepartment of Life Sciences and Systems Biology, University of Torino, Torino, ItalyDepartment of Chemistry, University of Copenhagen, Frederiksberg, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Chemistry, University of Copenhagen, Frederiksberg, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, DenmarkRecognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia.https://elifesciences.org/articles/33506Lotus japonicusNod factorRhizobiumLysM receptorsignallingsymbiosis |
| spellingShingle | Eiichi Murakami Jeryl Cheng Kira Gysel Zoltan Bozsoki Yasuyuki Kawaharada Christian Toftegaard Hjuler Kasper Kildegaard Sørensen Ke Tao Simon Kelly Francesco Venice Andrea Genre Mikkel Boas Thygesen Noor de Jong Maria Vinther Dorthe Bødker Jensen Knud Jørgen Jensen Michael Blaise Lene Heegaard Madsen Kasper Røjkjær Andersen Jens Stougaard Simona Radutoiu Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus eLife Lotus japonicus Nod factor Rhizobium LysM receptor signalling symbiosis |
| title | Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus |
| title_full | Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus |
| title_fullStr | Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus |
| title_full_unstemmed | Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus |
| title_short | Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus |
| title_sort | epidermal lysm receptor ensures robust symbiotic signalling in lotus japonicus |
| topic | Lotus japonicus Nod factor Rhizobium LysM receptor signalling symbiosis |
| url | https://elifesciences.org/articles/33506 |
| work_keys_str_mv | AT eiichimurakami epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT jerylcheng epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT kiragysel epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT zoltanbozsoki epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT yasuyukikawaharada epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT christiantoftegaardhjuler epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT kasperkildegaardsørensen epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT ketao epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT simonkelly epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT francescovenice epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT andreagenre epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT mikkelboasthygesen epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT noordejong epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT mariavinther epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT dorthebødkerjensen epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT knudjørgenjensen epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT michaelblaise epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT leneheegaardmadsen epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT kasperrøjkjærandersen epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT jensstougaard epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus AT simonaradutoiu epidermallysmreceptorensuresrobustsymbioticsignallinginlotusjaponicus |