Effect of a Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire
c-MYC (v-myelocytomatosis viral oncogene homologue) is a transcription factor that plays important role in many biological process including cell growth and differentiation, such as myogenesis and adipogenesis. In this study, we aimed to detect MYC gene polymorphisms, their genotype frequencies and...
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Asian-Australasian Association of Animal Production Societies
2015-11-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-28-11-1545.pdf |
| _version_ | 1819289452321701888 |
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| author | J. D. Oh E. S. Kim H. K. Lee K. D. Song |
| author_facet | J. D. Oh E. S. Kim H. K. Lee K. D. Song |
| author_sort | J. D. Oh |
| collection | DOAJ |
| description | c-MYC (v-myelocytomatosis viral oncogene homologue) is a transcription factor that plays important role in many biological process including cell growth and differentiation, such as myogenesis and adipogenesis. In this study, we aimed to detect MYC gene polymorphisms, their genotype frequencies and to determine associations between these polymorphisms and meat quality traits in Berkshire pigs. We identified a single nucleotide polymorphism (SNP) in intron 2 of MYC gene by Sanger sequencing, i.e., g.3350G>C (rs321898326), that is only found in Berkshire pigs, but not in other breeds including Duroc, Landrace, and Yorkshire pigs that were used in this study. Genotypes of total 378 Berkshire pigs (138 sows and 240 boars) were determined using Hha I restriction enzyme digestion after polymerase chain reaction. Observed allele frequencies of GG, GC, and CC genotypes were 0.399, 0.508, and 0.093 respectively. Statistical analysis indicated that the g.3350G>C polymorphism was significantly associated with pH45min and cooking loss (p<0.05), suggesting that g.3350G>C SNP can be used for pre-selection of pH45min and cooking loss traits in Berkshire pigs. |
| first_indexed | 2024-12-24T03:07:04Z |
| format | Article |
| id | doaj.art-f18780ab58f6466cb30be6ad0ce83fcb |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-24T03:07:04Z |
| publishDate | 2015-11-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-f18780ab58f6466cb30be6ad0ce83fcb2022-12-21T17:17:57ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172015-11-0128111545155010.5713/ajas.15.042523398Effect of a Gene Polymorphism (g.3350G>C) on Meat Quality Traits in BerkshireJ. D. OhE. S. Kim0H. K. LeeK. D. Song Department of Animal Science, Iowa State University, Ames, IA 50011, USAc-MYC (v-myelocytomatosis viral oncogene homologue) is a transcription factor that plays important role in many biological process including cell growth and differentiation, such as myogenesis and adipogenesis. In this study, we aimed to detect MYC gene polymorphisms, their genotype frequencies and to determine associations between these polymorphisms and meat quality traits in Berkshire pigs. We identified a single nucleotide polymorphism (SNP) in intron 2 of MYC gene by Sanger sequencing, i.e., g.3350G>C (rs321898326), that is only found in Berkshire pigs, but not in other breeds including Duroc, Landrace, and Yorkshire pigs that were used in this study. Genotypes of total 378 Berkshire pigs (138 sows and 240 boars) were determined using Hha I restriction enzyme digestion after polymerase chain reaction. Observed allele frequencies of GG, GC, and CC genotypes were 0.399, 0.508, and 0.093 respectively. Statistical analysis indicated that the g.3350G>C polymorphism was significantly associated with pH45min and cooking loss (p<0.05), suggesting that g.3350G>C SNP can be used for pre-selection of pH45min and cooking loss traits in Berkshire pigs.http://www.ajas.info/upload/pdf/ajas-28-11-1545.pdfBerkshireMeat Quality Traitsv-Myelocytomatosis Viral Oncogene HomologueSingle Nucleotide PolymorphismAssociation Analysis |
| spellingShingle | J. D. Oh E. S. Kim H. K. Lee K. D. Song Effect of a Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire Asian-Australasian Journal of Animal Sciences Berkshire Meat Quality Traits v-Myelocytomatosis Viral Oncogene Homologue Single Nucleotide Polymorphism Association Analysis |
| title | Effect of a Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire |
| title_full | Effect of a Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire |
| title_fullStr | Effect of a Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire |
| title_full_unstemmed | Effect of a Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire |
| title_short | Effect of a Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire |
| title_sort | effect of a gene polymorphism g 3350g c on meat quality traits in berkshire |
| topic | Berkshire Meat Quality Traits v-Myelocytomatosis Viral Oncogene Homologue Single Nucleotide Polymorphism Association Analysis |
| url | http://www.ajas.info/upload/pdf/ajas-28-11-1545.pdf |
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