Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility.
Basophils co-express FcεRIα and CD49b, the α-2 chain of integrin-type receptor VLA-2 (α2β1), which recognizes type-1 collagen as a major natural ligand. The physiological relevance of this integrin for interactions with extracellular bone marrow matrix remains unknown. Herein, we examined the expres...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3785469?pdf=render |
| _version_ | 1811273643557126144 |
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| author | Salete Smaniotto Elke Schneider Nicolas Goudin Rachel Bricard-Rignault François Machavoine Mireille Dardenne Michel Dy Wilson Savino |
| author_facet | Salete Smaniotto Elke Schneider Nicolas Goudin Rachel Bricard-Rignault François Machavoine Mireille Dardenne Michel Dy Wilson Savino |
| author_sort | Salete Smaniotto |
| collection | DOAJ |
| description | Basophils co-express FcεRIα and CD49b, the α-2 chain of integrin-type receptor VLA-2 (α2β1), which recognizes type-1 collagen as a major natural ligand. The physiological relevance of this integrin for interactions with extracellular bone marrow matrix remains unknown. Herein, we examined the expression of several receptors of this family by bone marrow-derived basophils sorted either ex-vivo or after culture with IL-3. Having established that both populations display CD49d, CD49e and CD49f (α-4, α-5 and α-6 integrins subunits, respectively), we addressed receptor functions by measuring migration, adhesion, proliferation and survival after interacting with matched natural ligands. Type I collagen, laminin and fibronectin promoted basophil migration/adhesion, the former being the most effective. None of these ligands affected basophil viability and expansion. Interactions between basophils and extracellular matrix are likely to play a role in situ, as supported by confocal 3D cell imaging of femoral bone marrow sections, which revealed basophils exclusively in type-1 collagen-enriched niches that contained likewise laminin and fibronectin. This is the first evidence for a structure/function relationship between basophils and extracellular matrix proteins inside the mouse bone marrow. |
| first_indexed | 2024-04-12T23:04:08Z |
| format | Article |
| id | doaj.art-f189ea45c38b4947a7a8cfcd32999c68 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T23:04:08Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f189ea45c38b4947a7a8cfcd32999c682022-12-22T03:12:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7029210.1371/journal.pone.0070292Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility.Salete SmaniottoElke SchneiderNicolas GoudinRachel Bricard-RignaultFrançois MachavoineMireille DardenneMichel DyWilson SavinoBasophils co-express FcεRIα and CD49b, the α-2 chain of integrin-type receptor VLA-2 (α2β1), which recognizes type-1 collagen as a major natural ligand. The physiological relevance of this integrin for interactions with extracellular bone marrow matrix remains unknown. Herein, we examined the expression of several receptors of this family by bone marrow-derived basophils sorted either ex-vivo or after culture with IL-3. Having established that both populations display CD49d, CD49e and CD49f (α-4, α-5 and α-6 integrins subunits, respectively), we addressed receptor functions by measuring migration, adhesion, proliferation and survival after interacting with matched natural ligands. Type I collagen, laminin and fibronectin promoted basophil migration/adhesion, the former being the most effective. None of these ligands affected basophil viability and expansion. Interactions between basophils and extracellular matrix are likely to play a role in situ, as supported by confocal 3D cell imaging of femoral bone marrow sections, which revealed basophils exclusively in type-1 collagen-enriched niches that contained likewise laminin and fibronectin. This is the first evidence for a structure/function relationship between basophils and extracellular matrix proteins inside the mouse bone marrow.http://europepmc.org/articles/PMC3785469?pdf=render |
| spellingShingle | Salete Smaniotto Elke Schneider Nicolas Goudin Rachel Bricard-Rignault François Machavoine Mireille Dardenne Michel Dy Wilson Savino Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility. PLoS ONE |
| title | Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility. |
| title_full | Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility. |
| title_fullStr | Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility. |
| title_full_unstemmed | Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility. |
| title_short | Mouse basophils reside in extracellular matrix-enriched bone marrow niches which control their motility. |
| title_sort | mouse basophils reside in extracellular matrix enriched bone marrow niches which control their motility |
| url | http://europepmc.org/articles/PMC3785469?pdf=render |
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