Monosaccharide composition analysis of polysaccharides in safflower by ion chromatography-pulsed amperometric detection(离子色谱-脉冲安培法分析西红花多糖的单糖组成)
采用水提醇沉法提取西红花球茎、柱头、花瓣、侧芽等部位的粗多糖，离心干燥得到的水溶性粗多糖在沸水浴条件下经硫酸水解后，通过离子色谱-脉冲安培法测定西红花不同部位多糖的单糖组成.该方法简单高效，灵敏度高，选择性好，不需要气相色谱和毛细管电泳所需的衍生化操作.在优化条件下，测得西红花多糖中含有岩藻糖、鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖和果糖7种单糖.采用此方法，各单糖检测的线性范围在0.02~20 mg • L-1, 线性相关系数均大于0.999 2，加标回收率在87.2%~111.6%，相对标准偏差在1.26%~3.61%，检出限在3.70~11.35 µg • L-1....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2015-05-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2015.03.016 |
| _version_ | 1797235979697782784 |
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| author | RAOJunfeng(饶君凤) LYUWeide(吕伟德) ZHANGZhuoliang(章卓梁) ZHOUGuangli(周光理) ZHUZuoyi(朱作艺) HEJie(贺婕) |
| author_facet | RAOJunfeng(饶君凤) LYUWeide(吕伟德) ZHANGZhuoliang(章卓梁) ZHOUGuangli(周光理) ZHUZuoyi(朱作艺) HEJie(贺婕) |
| author_sort | RAOJunfeng(饶君凤) |
| collection | DOAJ |
| description | 采用水提醇沉法提取西红花球茎、柱头、花瓣、侧芽等部位的粗多糖，离心干燥得到的水溶性粗多糖在沸水浴条件下经硫酸水解后，通过离子色谱-脉冲安培法测定西红花不同部位多糖的单糖组成.该方法简单高效，灵敏度高，选择性好，不需要气相色谱和毛细管电泳所需的衍生化操作.在优化条件下，测得西红花多糖中含有岩藻糖、鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖和果糖7种单糖.采用此方法，各单糖检测的线性范围在0.02~20 mg • L-1, 线性相关系数均大于0.999 2，加标回收率在87.2%~111.6%，相对标准偏差在1.26%~3.61%，检出限在3.70~11.35 µg • L-1. |
| first_indexed | 2024-04-24T16:56:34Z |
| format | Article |
| id | doaj.art-f18aa1b7fff44af7987db1a8f8f959fa |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:56:34Z |
| publishDate | 2015-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-f18aa1b7fff44af7987db1a8f8f959fa2024-03-29T01:58:34ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972015-05-0142333033310.3785/j.issn.1008-9497.2015.03.016Monosaccharide composition analysis of polysaccharides in safflower by ion chromatography-pulsed amperometric detection(离子色谱-脉冲安培法分析西红花多糖的单糖组成)RAOJunfeng(饶君凤)0LYUWeide(吕伟德)1ZHANGZhuoliang(章卓梁)2ZHOUGuangli(周光理)3ZHUZuoyi(朱作艺)4HEJie(贺婕)5 1.Hangzhou Vocational and Technical College, Hangzhou 310018, China( 1.杭州职业技术学院，浙江 杭州 310018) 1.Hangzhou Vocational and Technical College, Hangzhou 310018, China( 1.杭州职业技术学院，浙江 杭州 310018) 2.Agricultural Public Service Station of Dongqiao Town, Fuyang 311404, Zhejiang Province, China( 2.富阳洞桥镇农业公共服务站，浙江 富阳 311404) 1.Hangzhou Vocational and Technical College, Hangzhou 310018, China( 1.杭州职业技术学院，浙江 杭州 310018) 3.Department of Chemistry, Zhejiang University, Hangzhou 310028, China( 3.浙江大学化学系，浙江 杭州 310028) 3.Department of Chemistry, Zhejiang University, Hangzhou 310028, China( 3.浙江大学化学系，浙江 杭州 310028)采用水提醇沉法提取西红花球茎、柱头、花瓣、侧芽等部位的粗多糖，离心干燥得到的水溶性粗多糖在沸水浴条件下经硫酸水解后，通过离子色谱-脉冲安培法测定西红花不同部位多糖的单糖组成.该方法简单高效，灵敏度高，选择性好，不需要气相色谱和毛细管电泳所需的衍生化操作.在优化条件下，测得西红花多糖中含有岩藻糖、鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖和果糖7种单糖.采用此方法，各单糖检测的线性范围在0.02~20 mg • L-1, 线性相关系数均大于0.999 2，加标回收率在87.2%~111.6%，相对标准偏差在1.26%~3.61%，检出限在3.70~11.35 µg • L-1.https://doi.org/10.3785/j.issn.1008-9497.2015.03.016西红花多糖离子色谱-脉冲安培法单糖组成 |
| spellingShingle | RAOJunfeng(饶君凤) LYUWeide(吕伟德) ZHANGZhuoliang(章卓梁) ZHOUGuangli(周光理) ZHUZuoyi(朱作艺) HEJie(贺婕) Monosaccharide composition analysis of polysaccharides in safflower by ion chromatography-pulsed amperometric detection(离子色谱-脉冲安培法分析西红花多糖的单糖组成) Zhejiang Daxue xuebao. Lixue ban 西红花 多糖 离子色谱-脉冲安培法 单糖组成 |
| title | Monosaccharide composition analysis of polysaccharides in safflower by ion chromatography-pulsed amperometric detection(离子色谱-脉冲安培法分析西红花多糖的单糖组成) |
| title_full | Monosaccharide composition analysis of polysaccharides in safflower by ion chromatography-pulsed amperometric detection(离子色谱-脉冲安培法分析西红花多糖的单糖组成) |
| title_fullStr | Monosaccharide composition analysis of polysaccharides in safflower by ion chromatography-pulsed amperometric detection(离子色谱-脉冲安培法分析西红花多糖的单糖组成) |
| title_full_unstemmed | Monosaccharide composition analysis of polysaccharides in safflower by ion chromatography-pulsed amperometric detection(离子色谱-脉冲安培法分析西红花多糖的单糖组成) |
| title_short | Monosaccharide composition analysis of polysaccharides in safflower by ion chromatography-pulsed amperometric detection(离子色谱-脉冲安培法分析西红花多糖的单糖组成) |
| title_sort | monosaccharide composition analysis of polysaccharides in safflower by ion chromatography pulsed amperometric detection 离子色谱 脉冲安培法分析西红花多糖的单糖组成 |
| topic | 西红花 多糖 离子色谱-脉冲安培法 单糖组成 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2015.03.016 |
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