Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem.
BACKGROUND: The caudal brainstem plays an important role in short-term satiation and in the control of meal termination. Meal-related stimuli sensed by the gastrointestinal (GI) tract are transmitted to the area postrema (AP) via the bloodstream, or to the nucleus tractus solitarii (NTS) via the vag...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2813867?pdf=render |
| _version_ | 1818700897652310016 |
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| author | Jessica Schwarz Jasmine Burguet Olivier Rampin Gilles Fromentin Philippe Andrey Daniel Tomé Yves Maurin Nicolas Darcel |
| author_facet | Jessica Schwarz Jasmine Burguet Olivier Rampin Gilles Fromentin Philippe Andrey Daniel Tomé Yves Maurin Nicolas Darcel |
| author_sort | Jessica Schwarz |
| collection | DOAJ |
| description | BACKGROUND: The caudal brainstem plays an important role in short-term satiation and in the control of meal termination. Meal-related stimuli sensed by the gastrointestinal (GI) tract are transmitted to the area postrema (AP) via the bloodstream, or to the nucleus tractus solitarii (NTS) via the vagus nerve. Little is known about the encoding of macronutrient-specific signals in the caudal brainstem. We hypothesized that sucrose and casein peptone activate spatially distinct sub-populations of NTS neurons and thus characterized the latter using statistical three-dimensional modeling. METHODOLOGY/PRINCIPAL FINDINGS: Using immunolabeling of the proto-oncogene Fos as a marker of neuronal activity, in combination with a statistical three-dimensional modeling approach, we have shown that NTS neurons activated by sucrose or peptone gavage occupy distinct, although partially overlapping, positions. Specifically, when compared to their homologues in peptone-treated mice, three-dimensional models calculated from neuronal density maps following sucrose gavage showed that Fos-positive neurons occupy a more lateral position at the rostral end of the NTS, and a more dorsal position at the caudal end. CONCLUSION/SIGNIFICANCE: To our knowledge, this is the first time that subpopulations of NTS neurons have be distinguished according to the spatial organization of their functional response. Such neuronal activity patterns may be of particular relevance to understanding the mechanisms that support the central encoding of signals related to the presence of macronutrients in the GI tract during digestion. Finally, this finding also illustrates the usefulness of statistical three-dimensional modeling to functional neuroanatomical studies. |
| first_indexed | 2024-12-17T15:12:15Z |
| format | Article |
| id | doaj.art-f18ac2b09af946aa94c97fc0c9d831a3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T15:12:15Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f18ac2b09af946aa94c97fc0c9d831a32022-12-21T21:43:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0152e897410.1371/journal.pone.0008974Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem.Jessica SchwarzJasmine BurguetOlivier RampinGilles FromentinPhilippe AndreyDaniel ToméYves MaurinNicolas DarcelBACKGROUND: The caudal brainstem plays an important role in short-term satiation and in the control of meal termination. Meal-related stimuli sensed by the gastrointestinal (GI) tract are transmitted to the area postrema (AP) via the bloodstream, or to the nucleus tractus solitarii (NTS) via the vagus nerve. Little is known about the encoding of macronutrient-specific signals in the caudal brainstem. We hypothesized that sucrose and casein peptone activate spatially distinct sub-populations of NTS neurons and thus characterized the latter using statistical three-dimensional modeling. METHODOLOGY/PRINCIPAL FINDINGS: Using immunolabeling of the proto-oncogene Fos as a marker of neuronal activity, in combination with a statistical three-dimensional modeling approach, we have shown that NTS neurons activated by sucrose or peptone gavage occupy distinct, although partially overlapping, positions. Specifically, when compared to their homologues in peptone-treated mice, three-dimensional models calculated from neuronal density maps following sucrose gavage showed that Fos-positive neurons occupy a more lateral position at the rostral end of the NTS, and a more dorsal position at the caudal end. CONCLUSION/SIGNIFICANCE: To our knowledge, this is the first time that subpopulations of NTS neurons have be distinguished according to the spatial organization of their functional response. Such neuronal activity patterns may be of particular relevance to understanding the mechanisms that support the central encoding of signals related to the presence of macronutrients in the GI tract during digestion. Finally, this finding also illustrates the usefulness of statistical three-dimensional modeling to functional neuroanatomical studies.http://europepmc.org/articles/PMC2813867?pdf=render |
| spellingShingle | Jessica Schwarz Jasmine Burguet Olivier Rampin Gilles Fromentin Philippe Andrey Daniel Tomé Yves Maurin Nicolas Darcel Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem. PLoS ONE |
| title | Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem. |
| title_full | Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem. |
| title_fullStr | Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem. |
| title_full_unstemmed | Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem. |
| title_short | Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem. |
| title_sort | three dimensional macronutrient associated fos expression patterns in the mouse brainstem |
| url | http://europepmc.org/articles/PMC2813867?pdf=render |
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