Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast Cells
Background. Root canal obturation is performed by gutta-percha cones and sealer. Therefore, these materials, specially sealers, must be biocompatible. This study investigated the cytotoxicity and mineralization activity of two calcium silicate-based sealers (Endoseal MTA and Ceraseal) and an epoxy r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2023-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2023/4376579 |
| _version_ | 1797809202351046656 |
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| author | Mohammad Shokrzadeh Farzaneh Sadat Motafeghi Anahita Lotfizadeh Mohammad Ghorbani Azam Haddadi Kohsar |
| author_facet | Mohammad Shokrzadeh Farzaneh Sadat Motafeghi Anahita Lotfizadeh Mohammad Ghorbani Azam Haddadi Kohsar |
| author_sort | Mohammad Shokrzadeh |
| collection | DOAJ |
| description | Background. Root canal obturation is performed by gutta-percha cones and sealer. Therefore, these materials, specially sealers, must be biocompatible. This study investigated the cytotoxicity and mineralization activity of two calcium silicate-based sealers (Endoseal MTA and Ceraseal) and an epoxy resin-based sealer (AH26). Materials and Methods. In this experiment, the cytotoxicity of Endoseal MTA, Ceraseal, and AH26 on human gingival fibroblast cells was examined using Methyl-Thiazol-Tetrazolium assay at time intervals of 24, 48, 72, and 120 hr. The mineralization activity of sealers was evaluated by Alizarin red staining assay. Prism, ver.3, software was used to perform statistical tests. One-way analysis of variance analysis, followed by Tukey’s test, was used to determine the group differences. P-values < 0.05 were considered statistically significant. Results. Cytotoxicity of sealers decreased gradually (P<0.0001). AH26 showed the highest level of cytotoxicity (P<0.001). In terms of cytotoxicity, no considerable differences were observed between the two-calcium silicate-based sealers (P>0.05). AH26 showed the lowest mineralization activity (P<0.0001). Among the calcium silicate-based sealers, mineralization and formation of calcium nodules were more frequently observed in the Endoseal MTA group (P<0.001). Conclusion. The examined calcium silicate-based sealers had less cytotoxicity and higher mineralization activity than the resin-based sealer (AH26). There was negligible difference between the cytotoxicity of the two-calcium silicate-based, but the cell mineralization caused by Endoseal MTA was higher. |
| first_indexed | 2024-03-13T06:49:00Z |
| format | Article |
| id | doaj.art-f18b29af833d4dd68a4f6835cba21fa6 |
| institution | Directory Open Access Journal |
| issn | 1687-8736 |
| language | English |
| last_indexed | 2024-03-13T06:49:00Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj.art-f18b29af833d4dd68a4f6835cba21fa62023-06-08T00:00:04ZengHindawi LimitedInternational Journal of Dentistry1687-87362023-01-01202310.1155/2023/4376579Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast CellsMohammad Shokrzadeh0Farzaneh Sadat Motafeghi1Anahita Lotfizadeh2Mohammad Ghorbani3Azam Haddadi Kohsar4Department of Toxicology-PharmacologyDepartment of Toxicology-PharmacologyStudent Research CommitteeDentistDepartment of EndodonticsBackground. Root canal obturation is performed by gutta-percha cones and sealer. Therefore, these materials, specially sealers, must be biocompatible. This study investigated the cytotoxicity and mineralization activity of two calcium silicate-based sealers (Endoseal MTA and Ceraseal) and an epoxy resin-based sealer (AH26). Materials and Methods. In this experiment, the cytotoxicity of Endoseal MTA, Ceraseal, and AH26 on human gingival fibroblast cells was examined using Methyl-Thiazol-Tetrazolium assay at time intervals of 24, 48, 72, and 120 hr. The mineralization activity of sealers was evaluated by Alizarin red staining assay. Prism, ver.3, software was used to perform statistical tests. One-way analysis of variance analysis, followed by Tukey’s test, was used to determine the group differences. P-values < 0.05 were considered statistically significant. Results. Cytotoxicity of sealers decreased gradually (P<0.0001). AH26 showed the highest level of cytotoxicity (P<0.001). In terms of cytotoxicity, no considerable differences were observed between the two-calcium silicate-based sealers (P>0.05). AH26 showed the lowest mineralization activity (P<0.0001). Among the calcium silicate-based sealers, mineralization and formation of calcium nodules were more frequently observed in the Endoseal MTA group (P<0.001). Conclusion. The examined calcium silicate-based sealers had less cytotoxicity and higher mineralization activity than the resin-based sealer (AH26). There was negligible difference between the cytotoxicity of the two-calcium silicate-based, but the cell mineralization caused by Endoseal MTA was higher.http://dx.doi.org/10.1155/2023/4376579 |
| spellingShingle | Mohammad Shokrzadeh Farzaneh Sadat Motafeghi Anahita Lotfizadeh Mohammad Ghorbani Azam Haddadi Kohsar Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast Cells International Journal of Dentistry |
| title | Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast Cells |
| title_full | Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast Cells |
| title_fullStr | Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast Cells |
| title_full_unstemmed | Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast Cells |
| title_short | Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast Cells |
| title_sort | cytotoxicity and mineralization activity of calcium silicate based root canal sealers compared to conventional resin based sealer in human gingival fibroblast cells |
| url | http://dx.doi.org/10.1155/2023/4376579 |
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