Quercetin affects membrane lipids and apoptosis in three-dimensional fibroblast cultures
The polyphenol quercetin is associated with numerous beneficial health effects in various pathologies such as oxidative stress, neurodegenerative disorders, inflammation, neoplastic processes, age-related diseases, and so on. However, the molecular mechanisms underlying the effects of quercetin and...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2021.1939785 |
| _version_ | 1819144239808774144 |
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| author | Albena Momchilova Tania Markovska Georgi Georgiev Stefan Pankov Galya Staneva Diana Petkova Plamen Krastev Adriana Pinkas Roumen Pankov |
| author_facet | Albena Momchilova Tania Markovska Georgi Georgiev Stefan Pankov Galya Staneva Diana Petkova Plamen Krastev Adriana Pinkas Roumen Pankov |
| author_sort | Albena Momchilova |
| collection | DOAJ |
| description | The polyphenol quercetin is associated with numerous beneficial health effects in various pathologies such as oxidative stress, neurodegenerative disorders, inflammation, neoplastic processes, age-related diseases, and so on. However, the molecular mechanisms underlying the effects of quercetin and its implication in the pharmaceutical practice require additional clarification. In this study we analyzed the biochemical mechanisms of quercetin effect on membrane lipids of fibroblasts cultured as three-dimensional (3D) cultures, which resemble living tissues more adequately compared to cellular monolayers. Quercetin treatment of 3D fibroblasts induced alterations of the plasma membrane phospholipid and fatty acid composition as well as in the phospholipid metabolizing enzymes sphingomyelinase and phospholipase A2. The level of sphingomyelin (SM), which acts as endogenous membrane antioxidant, was elevated due to quercetin action. Incubation of 3D fibroblasts with quercetin induced augmentation in the saturated fatty acids and reduction of all polyunsaturated fatty acids in the membrane phospholipid molecules of the plasma membranes, indicating an additional increase of the resistance to oxidative damage. Quercetin treatment reduced cholesterol susceptibility to oxidation, a phenomenon which is probably related to the observed increase in the level of SM. In addition, Western blot analysis showed augmented phosphorylation of Akt and expression of Bcl-2 in quercetin-treated cell, indicating that apoptosis was suppressed by quercetin. In conclusion, the reported results contribute to a better understanding of the beneficial effects of quercetin on the structure and functions of plasma membranes of 3D cell cultures, which are a more adequate model of living tissues. |
| first_indexed | 2024-12-22T12:38:59Z |
| format | Article |
| id | doaj.art-f190476ff2c4490987cb9e21576d6948 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-22T12:38:59Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-f190476ff2c4490987cb9e21576d69482022-12-21T18:25:29ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302021-01-0135194395210.1080/13102818.2021.19397851939785Quercetin affects membrane lipids and apoptosis in three-dimensional fibroblast culturesAlbena Momchilova0Tania Markovska1Georgi Georgiev2Stefan Pankov3Galya Staneva4Diana Petkova5Plamen Krastev6Adriana Pinkas7Roumen Pankov8Department of Lipid-Protein Interactions, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of SciencesDepartment of Lipid-Protein Interactions, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of SciencesDepartment of Cytology, Histology and Embryology, Faculty of Biology, Sofia UniversityDepartment of Lipid-Protein Interactions, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of SciencesDepartment of Lipid-Protein Interactions, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of SciencesDepartment of Lipid-Protein Interactions, Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of SciencesCardiology Clinic, University Hospital “St. Ekaterina”Director STEP/CSTEP, Office of Continuing Education, Suffolk County Community CollegeDepartment of Cytology, Histology and Embryology, Faculty of Biology, Sofia UniversityThe polyphenol quercetin is associated with numerous beneficial health effects in various pathologies such as oxidative stress, neurodegenerative disorders, inflammation, neoplastic processes, age-related diseases, and so on. However, the molecular mechanisms underlying the effects of quercetin and its implication in the pharmaceutical practice require additional clarification. In this study we analyzed the biochemical mechanisms of quercetin effect on membrane lipids of fibroblasts cultured as three-dimensional (3D) cultures, which resemble living tissues more adequately compared to cellular monolayers. Quercetin treatment of 3D fibroblasts induced alterations of the plasma membrane phospholipid and fatty acid composition as well as in the phospholipid metabolizing enzymes sphingomyelinase and phospholipase A2. The level of sphingomyelin (SM), which acts as endogenous membrane antioxidant, was elevated due to quercetin action. Incubation of 3D fibroblasts with quercetin induced augmentation in the saturated fatty acids and reduction of all polyunsaturated fatty acids in the membrane phospholipid molecules of the plasma membranes, indicating an additional increase of the resistance to oxidative damage. Quercetin treatment reduced cholesterol susceptibility to oxidation, a phenomenon which is probably related to the observed increase in the level of SM. In addition, Western blot analysis showed augmented phosphorylation of Akt and expression of Bcl-2 in quercetin-treated cell, indicating that apoptosis was suppressed by quercetin. In conclusion, the reported results contribute to a better understanding of the beneficial effects of quercetin on the structure and functions of plasma membranes of 3D cell cultures, which are a more adequate model of living tissues.http://dx.doi.org/10.1080/13102818.2021.1939785quercetinphospholipidsfatty acidssphingomyelincholesterolapoptosis |
| spellingShingle | Albena Momchilova Tania Markovska Georgi Georgiev Stefan Pankov Galya Staneva Diana Petkova Plamen Krastev Adriana Pinkas Roumen Pankov Quercetin affects membrane lipids and apoptosis in three-dimensional fibroblast cultures Biotechnology & Biotechnological Equipment quercetin phospholipids fatty acids sphingomyelin cholesterol apoptosis |
| title | Quercetin affects membrane lipids and apoptosis in three-dimensional fibroblast cultures |
| title_full | Quercetin affects membrane lipids and apoptosis in three-dimensional fibroblast cultures |
| title_fullStr | Quercetin affects membrane lipids and apoptosis in three-dimensional fibroblast cultures |
| title_full_unstemmed | Quercetin affects membrane lipids and apoptosis in three-dimensional fibroblast cultures |
| title_short | Quercetin affects membrane lipids and apoptosis in three-dimensional fibroblast cultures |
| title_sort | quercetin affects membrane lipids and apoptosis in three dimensional fibroblast cultures |
| topic | quercetin phospholipids fatty acids sphingomyelin cholesterol apoptosis |
| url | http://dx.doi.org/10.1080/13102818.2021.1939785 |
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