Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation
Background: Liver cancer is the second most common reason for cancer-related death globally, and thus, a major public health challenge. Plant-based drugs are promising therapeutic agents against cancers. Basil and Dracocephalum kotschyi extracts also exhibit therapeutic impact on various cancers. To...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences and Health Services
2019-02-01
|
| Series: | Research in Molecular Medicine |
| Subjects: | |
| Online Access: | http://rmm.mazums.ac.ir/article-1-308-en.html |
| _version_ | 1818294283057233920 |
|---|---|
| author | Mansour Moghimi Sara Rashidian Farinaz Khosravian Nasrin Hadi |
| author_facet | Mansour Moghimi Sara Rashidian Farinaz Khosravian Nasrin Hadi |
| author_sort | Mansour Moghimi |
| collection | DOAJ |
| description | Background: Liver cancer is the second most common reason for cancer-related death globally, and thus, a major public health challenge. Plant-based drugs are promising therapeutic agents against cancers. Basil and Dracocephalum kotschyi extracts also exhibit therapeutic impact on various cancers. To assess the effects of the extract of Basil and Dracocephalum kotschyi on cell proliferation and BCL2 expression in the HepG2 cell line.
Materials and methods: IIn this experimental study, the HepG2 cell line was selected for treatment with the alcoholic extracts of Basil and Dracocephalum kotschyi. Cell proliferation was examined in the presence of various concentrations of the Basil and Dracocephalum kotschyi extracts by MTT assay. Moreover, the effect of the alcoholic extracts of Basil and Dracocephalum kotschyi on BCL2 expression was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR).
Results: Cell proliferation analyses showed anticancer characteristics of the alcoholic extracts of Basil and Dracocephalum kotschyi. In addition, treatment with the extracts of Basil and Dracocephalum kotschyi led to the decreased expression of BCL2 in HepG2 cell line.
Conclusion: These findings indicated that Basil and Dracocephalum kotschyi are promising candidates for future anticancer research. |
| first_indexed | 2024-12-13T03:29:17Z |
| format | Article |
| id | doaj.art-f1910a8b03924eabae574945f6183cff |
| institution | Directory Open Access Journal |
| issn | 2322-1348 2322-133X |
| language | English |
| last_indexed | 2024-12-13T03:29:17Z |
| publishDate | 2019-02-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-f1910a8b03924eabae574945f6183cff2022-12-22T00:01:12ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2019-02-01712634Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferationMansour Moghimi0Sara Rashidian1Farinaz Khosravian2Nasrin Hadi3 Clinical and Surgical pathology, Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Medical Immunology Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Molecular, Cellular and Genetics Research Center, Medical Genetics Research Center of GENOME, Isfahan, Iran. Medical Genetics Research Center of Genome, Mohtasham St., Isfahan, Iran Background: Liver cancer is the second most common reason for cancer-related death globally, and thus, a major public health challenge. Plant-based drugs are promising therapeutic agents against cancers. Basil and Dracocephalum kotschyi extracts also exhibit therapeutic impact on various cancers. To assess the effects of the extract of Basil and Dracocephalum kotschyi on cell proliferation and BCL2 expression in the HepG2 cell line. Materials and methods: IIn this experimental study, the HepG2 cell line was selected for treatment with the alcoholic extracts of Basil and Dracocephalum kotschyi. Cell proliferation was examined in the presence of various concentrations of the Basil and Dracocephalum kotschyi extracts by MTT assay. Moreover, the effect of the alcoholic extracts of Basil and Dracocephalum kotschyi on BCL2 expression was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Cell proliferation analyses showed anticancer characteristics of the alcoholic extracts of Basil and Dracocephalum kotschyi. In addition, treatment with the extracts of Basil and Dracocephalum kotschyi led to the decreased expression of BCL2 in HepG2 cell line. Conclusion: These findings indicated that Basil and Dracocephalum kotschyi are promising candidates for future anticancer research.http://rmm.mazums.ac.ir/article-1-308-en.htmlBasilBCL2Dracocephalum kotschyiHepG2Liver cancer. |
| spellingShingle | Mansour Moghimi Sara Rashidian Farinaz Khosravian Nasrin Hadi Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation Research in Molecular Medicine Basil BCL2 Dracocephalum kotschyi HepG2 Liver cancer. |
| title | Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation |
| title_full | Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation |
| title_fullStr | Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation |
| title_full_unstemmed | Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation |
| title_short | Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation |
| title_sort | basil and dracocephalum kotschyi alcoholic extracts affect bcl2 expression and hepg2 cell proliferation |
| topic | Basil BCL2 Dracocephalum kotschyi HepG2 Liver cancer. |
| url | http://rmm.mazums.ac.ir/article-1-308-en.html |
| work_keys_str_mv | AT mansourmoghimi basilanddracocephalumkotschyialcoholicextractsaffectbcl2expressionandhepg2cellproliferation AT sararashidian basilanddracocephalumkotschyialcoholicextractsaffectbcl2expressionandhepg2cellproliferation AT farinazkhosravian basilanddracocephalumkotschyialcoholicextractsaffectbcl2expressionandhepg2cellproliferation AT nasrinhadi basilanddracocephalumkotschyialcoholicextractsaffectbcl2expressionandhepg2cellproliferation |