Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method
White muscle disease (WMD) and/or nutritional muscular dystrophy is defined as a disease with a generally acute course that results in degeneration and necrosis of the heart muscle. In this study, it was aimed to reveal local tissue expressions of acute phase proteins such as C–reactive protein (CR...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia
2024-01-01
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| Series: | Revista Científica |
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| Online Access: | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/41451 |
| _version_ | 1797368354270347264 |
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| author | Ozhan Karatas Gokhan Akcakavak |
| author_facet | Ozhan Karatas Gokhan Akcakavak |
| author_sort | Ozhan Karatas |
| collection | DOAJ |
| description |
White muscle disease (WMD) and/or nutritional muscular dystrophy is defined as a disease with a generally acute course that results in degeneration and necrosis of the heart muscle. In this study, it was aimed to reveal local tissue expressions of acute phase proteins such as C–reactive protein (CRP), Serum amyloid–A (SAA) and Haptoglobin (Hp) in lambs with WMD. The study material consisted of 27, one–six months old lamb heart tissues, 6 of which were healthy and 21 with WMD. The lambs were necropsied, and the relevant heart samples were taken into neutral formaldehyde. Afterwards, paraffin blocks were obtained by going through routine tissue follow–up processes. Sections were taken from paraffin blocks and stained with Hematoxylin–Eosin (H–E) and Immunohistochemical methods. Histopathologically, control groups exhibited normal histology. Hyaline degeneration, Zenker necrosis, calcification, inflammatory cell infiltration and an increase in connective tissue were detected in the heart tissues of lambs with WMD. Lambs with WMD had significantly increased CRP and SAA proteins compared to control (P<0.01). However, there was no difference between the groups in Hp (P>0.05). In conclusion, local tissue expressions of CRP, SAA and Hp in lambs with WMD were identified immunohistochemically for the first time. It is possible to say that CRP and SAA may play important roles in the pathophysiology of WMD and that CRP and SAA may provide more sensitive results in the diagnosis and prognosis of the disease.
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| first_indexed | 2024-03-08T17:31:16Z |
| format | Article |
| id | doaj.art-f19331773e284cf794967f07395aa29a |
| institution | Directory Open Access Journal |
| issn | 0798-2259 2521-9715 |
| language | English |
| last_indexed | 2024-03-08T17:31:16Z |
| publishDate | 2024-01-01 |
| publisher | Universidad del Zulia |
| record_format | Article |
| series | Revista Científica |
| spelling | doaj.art-f19331773e284cf794967f07395aa29a2024-01-02T15:27:02ZengUniversidad del ZuliaRevista Científica0798-22592521-97152024-01-0134110.52973/rcfcv-e34313Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical methodOzhan Karatas0Gokhan Akcakavak1Cumhuriyet University, Faculty of Veterinary Medicine, Department of Pathology. Merkez, Sivas, TürkiyeYozgat Bozok University, Faculty of Veterinary Medicine, Department of Pathology. Sorgun, Yozgat, Türkiye White muscle disease (WMD) and/or nutritional muscular dystrophy is defined as a disease with a generally acute course that results in degeneration and necrosis of the heart muscle. In this study, it was aimed to reveal local tissue expressions of acute phase proteins such as C–reactive protein (CRP), Serum amyloid–A (SAA) and Haptoglobin (Hp) in lambs with WMD. The study material consisted of 27, one–six months old lamb heart tissues, 6 of which were healthy and 21 with WMD. The lambs were necropsied, and the relevant heart samples were taken into neutral formaldehyde. Afterwards, paraffin blocks were obtained by going through routine tissue follow–up processes. Sections were taken from paraffin blocks and stained with Hematoxylin–Eosin (H–E) and Immunohistochemical methods. Histopathologically, control groups exhibited normal histology. Hyaline degeneration, Zenker necrosis, calcification, inflammatory cell infiltration and an increase in connective tissue were detected in the heart tissues of lambs with WMD. Lambs with WMD had significantly increased CRP and SAA proteins compared to control (P<0.01). However, there was no difference between the groups in Hp (P>0.05). In conclusion, local tissue expressions of CRP, SAA and Hp in lambs with WMD were identified immunohistochemically for the first time. It is possible to say that CRP and SAA may play important roles in the pathophysiology of WMD and that CRP and SAA may provide more sensitive results in the diagnosis and prognosis of the disease. http://www.produccioncientificaluz.org/index.php/cientifica/article/view/41451White muscle diseaseimmunohistochemistryC–reactive proteinserum amyloid–Ahaptoglobin |
| spellingShingle | Ozhan Karatas Gokhan Akcakavak Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method Revista Científica White muscle disease immunohistochemistry C–reactive protein serum amyloid–A haptoglobin |
| title | Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method |
| title_full | Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method |
| title_fullStr | Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method |
| title_full_unstemmed | Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method |
| title_short | Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method |
| title_sort | evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method |
| topic | White muscle disease immunohistochemistry C–reactive protein serum amyloid–A haptoglobin |
| url | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/41451 |
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