In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice
Objective: To verify the trypanocidal effectiveness of aescin and aescin liposomes against Trypanosoma evansi in vitro and in vivo. Methods: Aescin and aescin liposomes were used in vitro on trypomastigotes at different concentrations (0.5%, 1.0% and 2.0%) and exposure times (0, 1, 3, 6 and 9 h). In...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-12-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S222116911530109X |
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| author | Matheus Dellaméa Baldissera Nathieli Bianchin Bottari Thirssa Helena Grando Roberto Christ Vianna Santos Ana Júlia Figueiró Dalcin Patrícia Gomes Renata Platcheck Raffin Carine Eloise Prestes Zimmerman Janio Morais Santurio Silvia Gonzalez Monteiro Aleksandro Schafer Da Silva |
| author_facet | Matheus Dellaméa Baldissera Nathieli Bianchin Bottari Thirssa Helena Grando Roberto Christ Vianna Santos Ana Júlia Figueiró Dalcin Patrícia Gomes Renata Platcheck Raffin Carine Eloise Prestes Zimmerman Janio Morais Santurio Silvia Gonzalez Monteiro Aleksandro Schafer Da Silva |
| author_sort | Matheus Dellaméa Baldissera |
| collection | DOAJ |
| description | Objective: To verify the trypanocidal effectiveness of aescin and aescin liposomes against Trypanosoma evansi in vitro and in vivo.
Methods: Aescin and aescin liposomes were used in vitro on trypomastigotes at different concentrations (0.5%, 1.0% and 2.0%) and exposure times (0, 1, 3, 6 and 9 h). In vivo tests were performed using mice as the experimental model. Trypanosome evansi infected mice were treated with aescin and aescin liposomes with doses of 60 and 100 mg/kg during 4 d.
Results: The three concentrations tested in free form and nanoencapsulated showed trypanocidal activity in vitro, completely eliminating the parasites in small concentration after 6 h of assay. Animals treated with aescin (100 mg/kg) and aescin liposomes (100 mg/kg) showed increase in longevity, however without curative effect.
Conclusions: Active compounds present in natural products, such as aescin, may potentiate the treatment of trypanosomosis when used in association with other trypanocidal drugs. |
| first_indexed | 2024-12-12T04:02:32Z |
| format | Article |
| id | doaj.art-f197587618c1412d8e659cd1874e2d78 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-12T04:02:32Z |
| publishDate | 2014-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-f197587618c1412d8e659cd1874e2d782022-12-22T00:38:56ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-12-0141294795110.12980/APJTB.4.2014APJTB-2014-0435In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental miceMatheus Dellaméa Baldissera0Nathieli Bianchin Bottari1Thirssa Helena Grando2Roberto Christ Vianna Santos3Ana Júlia Figueiró Dalcin4Patrícia Gomes5Renata Platcheck Raffin6Carine Eloise Prestes Zimmerman7Janio Morais Santurio8Silvia Gonzalez Monteiro9Aleksandro Schafer Da Silva10Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, BrazilLaboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, BrazilDepartment of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, BrazilLaboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, BrazilLaboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, BrazilLaboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, BrazilLaboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, BrazilDepartment of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, BrazilDepartment of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, BrazilDepartment of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, BrazilDepartment of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, BrazilObjective: To verify the trypanocidal effectiveness of aescin and aescin liposomes against Trypanosoma evansi in vitro and in vivo. Methods: Aescin and aescin liposomes were used in vitro on trypomastigotes at different concentrations (0.5%, 1.0% and 2.0%) and exposure times (0, 1, 3, 6 and 9 h). In vivo tests were performed using mice as the experimental model. Trypanosome evansi infected mice were treated with aescin and aescin liposomes with doses of 60 and 100 mg/kg during 4 d. Results: The three concentrations tested in free form and nanoencapsulated showed trypanocidal activity in vitro, completely eliminating the parasites in small concentration after 6 h of assay. Animals treated with aescin (100 mg/kg) and aescin liposomes (100 mg/kg) showed increase in longevity, however without curative effect. Conclusions: Active compounds present in natural products, such as aescin, may potentiate the treatment of trypanosomosis when used in association with other trypanocidal drugs.http://www.sciencedirect.com/science/article/pii/S222116911530109XkeywordsSurraNanotechnologyAesculus hippocastanumTrypanosome |
| spellingShingle | Matheus Dellaméa Baldissera Nathieli Bianchin Bottari Thirssa Helena Grando Roberto Christ Vianna Santos Ana Júlia Figueiró Dalcin Patrícia Gomes Renata Platcheck Raffin Carine Eloise Prestes Zimmerman Janio Morais Santurio Silvia Gonzalez Monteiro Aleksandro Schafer Da Silva In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice Asian Pacific Journal of Tropical Biomedicine keywords Surra Nanotechnology Aesculus hippocastanum Trypanosome |
| title | In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice |
| title_full | In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice |
| title_fullStr | In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice |
| title_full_unstemmed | In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice |
| title_short | In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi in experimental mice |
| title_sort | in vitro and in vivo trypanocidal action of aescin and aescin liposomes against trypanosoma evansi in experimental mice |
| topic | keywords Surra Nanotechnology Aesculus hippocastanum Trypanosome |
| url | http://www.sciencedirect.com/science/article/pii/S222116911530109X |
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