Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug <i>Riptortus pedestris</i> to Different Food Resources
The bean bug, <i>Riptortus pedestris</i> (Hemiptera: Heteroptera), poses a significant threat to soybean production, resulting in substantial crop losses. Throughout the soybean cultivation period, these insects probe and suck on various parts of plants, including leaves, pods, and beans...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/9/739 |
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| author | Ze-Long Zhang Xiao-Jing Wang Hai-Bin Lu Hai-Jian Huang |
| author_facet | Ze-Long Zhang Xiao-Jing Wang Hai-Bin Lu Hai-Jian Huang |
| author_sort | Ze-Long Zhang |
| collection | DOAJ |
| description | The bean bug, <i>Riptortus pedestris</i> (Hemiptera: Heteroptera), poses a significant threat to soybean production, resulting in substantial crop losses. Throughout the soybean cultivation period, these insects probe and suck on various parts of plants, including leaves, pods, and beans. However, the specific mechanisms by which they adapt to different food resources remain unknown. In this study, we conducted gut transcriptomic analyses of <i>R. pedestris</i> fed with soybean leaves, pods, and beans. A total of 798, 690, and 548 differently expressed genes (DEGs) were monitored in G—pod vs. G—leaf (comparison of insect feeding on pods and leaves), G—bean vs. G—leaf (comparison of insect feeding on beans and leaves), and G—pod vs. G—bean (comparison of insect feeding on pods and beans), respectively. When fed on pods and beans, there was a significant increase in the expression of digestive enzymes, particularly cathepsins, serine proteases, and lipases. Conversely, when soybean leaves were consumed, detoxification enzymes, such as ABC transporters and 4-coumarate-CoA ligase, exhibited higher expression. Our findings indicate that <i>R. pedestris</i> dynamically regulates different metabolic pathways to cope with varying food resources, which may contribute to the development of effective strategies for managing this pest. |
| first_indexed | 2024-03-10T22:38:43Z |
| format | Article |
| id | doaj.art-f19849392cc7477ab56be20d262918f1 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T22:38:43Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-f19849392cc7477ab56be20d262918f12023-11-19T11:15:14ZengMDPI AGInsects2075-44502023-08-0114973910.3390/insects14090739Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug <i>Riptortus pedestris</i> to Different Food ResourcesZe-Long Zhang0Xiao-Jing Wang1Hai-Bin Lu2Hai-Jian Huang3State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaThe bean bug, <i>Riptortus pedestris</i> (Hemiptera: Heteroptera), poses a significant threat to soybean production, resulting in substantial crop losses. Throughout the soybean cultivation period, these insects probe and suck on various parts of plants, including leaves, pods, and beans. However, the specific mechanisms by which they adapt to different food resources remain unknown. In this study, we conducted gut transcriptomic analyses of <i>R. pedestris</i> fed with soybean leaves, pods, and beans. A total of 798, 690, and 548 differently expressed genes (DEGs) were monitored in G—pod vs. G—leaf (comparison of insect feeding on pods and leaves), G—bean vs. G—leaf (comparison of insect feeding on beans and leaves), and G—pod vs. G—bean (comparison of insect feeding on pods and beans), respectively. When fed on pods and beans, there was a significant increase in the expression of digestive enzymes, particularly cathepsins, serine proteases, and lipases. Conversely, when soybean leaves were consumed, detoxification enzymes, such as ABC transporters and 4-coumarate-CoA ligase, exhibited higher expression. Our findings indicate that <i>R. pedestris</i> dynamically regulates different metabolic pathways to cope with varying food resources, which may contribute to the development of effective strategies for managing this pest.https://www.mdpi.com/2075-4450/14/9/739guttranscriptomicadaptation<i>Riptortus pedestris</i>food source |
| spellingShingle | Ze-Long Zhang Xiao-Jing Wang Hai-Bin Lu Hai-Jian Huang Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug <i>Riptortus pedestris</i> to Different Food Resources Insects gut transcriptomic adaptation <i>Riptortus pedestris</i> food source |
| title | Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug <i>Riptortus pedestris</i> to Different Food Resources |
| title_full | Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug <i>Riptortus pedestris</i> to Different Food Resources |
| title_fullStr | Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug <i>Riptortus pedestris</i> to Different Food Resources |
| title_full_unstemmed | Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug <i>Riptortus pedestris</i> to Different Food Resources |
| title_short | Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug <i>Riptortus pedestris</i> to Different Food Resources |
| title_sort | comparative transcriptomic analysis reveals adaptation mechanisms of bean bug i riptortus pedestris i to different food resources |
| topic | gut transcriptomic adaptation <i>Riptortus pedestris</i> food source |
| url | https://www.mdpi.com/2075-4450/14/9/739 |
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