Regulation of diet containing omega-3 polyunsaturated fatty acids on retinal neovascularization in mice
AIM: To study the regulation mechanism of diet containing omega-3 polyunsaturated fatty acids(ω-3 PUFAs)on retinal neovascularization in an oxygen-induced retinopathy(OIR)mouse model. <p>METHODS: Sixty C57BL /6J mice, seven-day-old, were classified into 3 groups: A the normal control group, B...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-01-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2018/1/201801006.pdf |
| Summary: | AIM: To study the regulation mechanism of diet containing omega-3 polyunsaturated fatty acids(ω-3 PUFAs)on retinal neovascularization in an oxygen-induced retinopathy(OIR)mouse model. <p>METHODS: Sixty C57BL /6J mice, seven-day-old, were classified into 3 groups: A the normal control group, B the OIR model group, C the ω-3 PUFAs diet group. Each group has twenty mice and separated fed by their lactating mice. The normal control group was fed in a standard atmosphere environment, B, C groups were first fed in a hyper-oxygen atmosphere of(75±2)% oxygen percentage for 5d, then continue fed in a standard atmosphere. The ω-3 PUFAs diet group was fed with dose base on their weight by 7.5mg/kg/d. All mice were sacrificed when they were seventeen-day-old, the relative neovascularization areas(NA)were calculated by fluorescein angiography on flat-mounted retina. The number of endothelial cell nuclei breaking through the inner linmiting membrane(ILM)was counted on hematoxylin and eosin-stained retinal section. The ω-3PUFAs/ω-6PUFAs relative amount and ratio was measured by GC-MS in the retina. A real-time PCR and Western Blot method were used to detect the mRNA, peroxisome proliferator-avtivated receptor–γ(RPAR-γ), vascular endothelial growth factor-A(VEGF-A)and vascular endothelial growth factor receptor 2(VEGFR-2)in the retina. <p>RESULTS: There was a significant different in all groups on the relative neovascularization areas and the number of endothelial cell nuclei breaking through the ILM(<i>F<sub>NA</sub></i>=20.45, <i>P</i><0.05; F<sub>ILM</sub>=48.66, <i>P</i><0.05). NA between Group A and B had a significant difference(<i>t</i>=8.64, <i>P</i><0.05), the same between Group C and B(<i>t</i>=8.91, <i>P</i><0.05). The cell nuclei breaking through ILM in Group A and B was significantly different(<i>t</i>=38.51, <i>P</i><0.05), the same in Group C and B(<i>t</i>=19.86, <i>P</i><0.05). For the relative contain in retina of ω-3PUFAs and ω-6PUFAs, there was a significant different among all groups(<i>F</i>=129.86, <i>F</i>=112.44; all <i>P</i><0.05). That of Group C was significant different than other two groups(<i>t</i>=23.15, 25.42; <i>t</i>=16.43, 11.95; <i>P</i><0.05). There were significant different among all groups on ω-3PUFAs/ω-6PUFAs ratio, retinal RPAR-γmRNA expression, retinal VEGF-A mRNA expression and VEGFR-2 mRNA expression(<i>F<sub>ω-3</sub><sub>/6</sub></i>=10.30, <i>F<sub>RPAR-γ</sub></i>=138.24, <i>F<sub>VEGF-A</sub></i>=69.12, <i>F<sub>VEGFR-2</sub></i>=52.45; <i>P</i><0.05). The ω-3PUFAs/ω-6PUFAs ratio of Group C was higher than that of Group B(<i>P</i><0.05). Compared to Group B, on one hand Group C had a higher expression(<i>P</i><0.05), on other hand Group C had a lower expression on VEGF-A mRNA and VEGFR-2 mRNA(<i>P</i><0.05). <p>CONCLUSION:The diet rich with ω-3 PUFAs uplifts the ω-3PUFAs/ω-6PUFAs ratio and activates RPAR-γ to lower expression of VEGF-A and VEGFR-2 to inhabit oxygen induced retinal neovascularization. |
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| ISSN: | 1672-5123 1672-5123 |