The microRNA-processing enzyme Dicer is essential for thyroid function.
Dicer is a type III ribonuclease required for the biogenesis of microRNAs (miRNAs), a class of small non-coding RNAs regulating gene expression at the post-transcriptional level. To explore the functional role of miRNAs in thyroid gland function, we generated a thyrocyte-specific Dicer conditional k...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3221669?pdf=render |
| _version_ | 1818842944192380928 |
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| author | Daniela Frezzetti Carla Reale Gaetano Calì Lucio Nitsch Henrik Fagman Ola Nilsson Marzia Scarfò Gabriella De Vita Roberto Di Lauro |
| author_facet | Daniela Frezzetti Carla Reale Gaetano Calì Lucio Nitsch Henrik Fagman Ola Nilsson Marzia Scarfò Gabriella De Vita Roberto Di Lauro |
| author_sort | Daniela Frezzetti |
| collection | DOAJ |
| description | Dicer is a type III ribonuclease required for the biogenesis of microRNAs (miRNAs), a class of small non-coding RNAs regulating gene expression at the post-transcriptional level. To explore the functional role of miRNAs in thyroid gland function, we generated a thyrocyte-specific Dicer conditional knockout mouse. Here we show that development and early differentiation of the thyroid gland are not affected by the absence of Dicer, while severe hypothyroidism gradually develops after birth, leading to reduced body weight and shortened life span. Histological and molecular characterization of knockout mice reveals a dramatic loss of the thyroid gland follicular architecture associated with functional aberrations and down-regulation of several differentiation markers. The data presented in this study show for the first time that an intact miRNAs processing machinery is essential for thyroid physiology, suggesting that deregulation of specific miRNAs could be also involved in human thyroid dysfunctions. |
| first_indexed | 2024-12-19T04:50:01Z |
| format | Article |
| id | doaj.art-f19d0f9c07544a639e62d978376ccd38 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T04:50:01Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f19d0f9c07544a639e62d978376ccd382022-12-21T20:35:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2764810.1371/journal.pone.0027648The microRNA-processing enzyme Dicer is essential for thyroid function.Daniela FrezzettiCarla RealeGaetano CalìLucio NitschHenrik FagmanOla NilssonMarzia ScarfòGabriella De VitaRoberto Di LauroDicer is a type III ribonuclease required for the biogenesis of microRNAs (miRNAs), a class of small non-coding RNAs regulating gene expression at the post-transcriptional level. To explore the functional role of miRNAs in thyroid gland function, we generated a thyrocyte-specific Dicer conditional knockout mouse. Here we show that development and early differentiation of the thyroid gland are not affected by the absence of Dicer, while severe hypothyroidism gradually develops after birth, leading to reduced body weight and shortened life span. Histological and molecular characterization of knockout mice reveals a dramatic loss of the thyroid gland follicular architecture associated with functional aberrations and down-regulation of several differentiation markers. The data presented in this study show for the first time that an intact miRNAs processing machinery is essential for thyroid physiology, suggesting that deregulation of specific miRNAs could be also involved in human thyroid dysfunctions.http://europepmc.org/articles/PMC3221669?pdf=render |
| spellingShingle | Daniela Frezzetti Carla Reale Gaetano Calì Lucio Nitsch Henrik Fagman Ola Nilsson Marzia Scarfò Gabriella De Vita Roberto Di Lauro The microRNA-processing enzyme Dicer is essential for thyroid function. PLoS ONE |
| title | The microRNA-processing enzyme Dicer is essential for thyroid function. |
| title_full | The microRNA-processing enzyme Dicer is essential for thyroid function. |
| title_fullStr | The microRNA-processing enzyme Dicer is essential for thyroid function. |
| title_full_unstemmed | The microRNA-processing enzyme Dicer is essential for thyroid function. |
| title_short | The microRNA-processing enzyme Dicer is essential for thyroid function. |
| title_sort | microrna processing enzyme dicer is essential for thyroid function |
| url | http://europepmc.org/articles/PMC3221669?pdf=render |
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