Effects of Crude <i>Fucus distichus</i> Subspecies <i>evanescens</i> Fucoidan Extract on Retinal Pigment Epithelium Cells―Implications for Use in Age-Related Macular Degeneration
Fucoidan extracts may have beneficial effects in age-related macular degeneration (AMD). Over-the-counter fucoidan preparations are generally undefined, crude extracts. In this study, we investigated the effect of a crude fucoidan extract from <i>Fucus distichus</i> subspecies <i>e...
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MDPI AG
2019-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/9/538 |
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| author | Kevin Rohwer Sandesh Neupane Kaya Saskia Bittkau Mayra Galarza Pérez Philipp Dörschmann Johann Roider Susanne Alban Alexa Klettner |
| author_facet | Kevin Rohwer Sandesh Neupane Kaya Saskia Bittkau Mayra Galarza Pérez Philipp Dörschmann Johann Roider Susanne Alban Alexa Klettner |
| author_sort | Kevin Rohwer |
| collection | DOAJ |
| description | Fucoidan extracts may have beneficial effects in age-related macular degeneration (AMD). Over-the-counter fucoidan preparations are generally undefined, crude extracts. In this study, we investigated the effect of a crude fucoidan extract from <i>Fucus distichus</i> subspecies <i>evanescens</i> (Fe) on the retinal pigment epithelium (RPE). Fe extract was investigated for chemical composition and molar mass. It was tested in primary RPE and RPE cell line ARPE19. Oxidative stress was induced with <i>tert</i>-butyl hydroperoxide, cell viability evaluated with MTT assay, VEGF secretion assessed in ELISA. Phagocytosis was evaluated in a fluorescence microscopic assay. Wound healing ability was tested in a scratch assay. Additionally, the inhibition of elastase and complement system by Fe extract was studied. The Fe extract contained about 61.9% fucose and high amounts of uronic acids (26.2%). The sulfate content was not as high as expected (6.9%). It was not toxic and not protective against oxidative stress. However, Fe extract was able to reduce VEGF secretion in ARPE19. Phagocytosis was also reduced. Concerning wound healing, a delay could be observed in higher concentrations. While some beneficial effects could be found, it seems to interfere with RPE function, which may reduce its beneficial effects in AMD treatment. |
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| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-12-10T06:34:42Z |
| publishDate | 2019-09-01 |
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| spelling | doaj.art-f1a4c2acf0c948e68a81a4cee14420fc2022-12-22T01:58:59ZengMDPI AGMarine Drugs1660-33972019-09-0117953810.3390/md17090538md17090538Effects of Crude <i>Fucus distichus</i> Subspecies <i>evanescens</i> Fucoidan Extract on Retinal Pigment Epithelium Cells―Implications for Use in Age-Related Macular DegenerationKevin Rohwer0Sandesh Neupane1Kaya Saskia Bittkau2Mayra Galarza Pérez3Philipp Dörschmann4Johann Roider5Susanne Alban6Alexa Klettner7Department of Ophthalmology, University Medical Center, University of Kiel, 24105 Kiel, GermanyDepartment of Pharmaceutical Biology, Pharmaceutical Institute, University of Kiel, 24105 Kiel, GermanyDepartment of Pharmaceutical Biology, Pharmaceutical Institute, University of Kiel, 24105 Kiel, GermanyDepartment of Pharmaceutical Biology, Pharmaceutical Institute, University of Kiel, 24105 Kiel, GermanyDepartment of Ophthalmology, University Medical Center, University of Kiel, 24105 Kiel, GermanyDepartment of Ophthalmology, University Medical Center, University of Kiel, 24105 Kiel, GermanyDepartment of Pharmaceutical Biology, Pharmaceutical Institute, University of Kiel, 24105 Kiel, GermanyDepartment of Ophthalmology, University Medical Center, University of Kiel, 24105 Kiel, GermanyFucoidan extracts may have beneficial effects in age-related macular degeneration (AMD). Over-the-counter fucoidan preparations are generally undefined, crude extracts. In this study, we investigated the effect of a crude fucoidan extract from <i>Fucus distichus</i> subspecies <i>evanescens</i> (Fe) on the retinal pigment epithelium (RPE). Fe extract was investigated for chemical composition and molar mass. It was tested in primary RPE and RPE cell line ARPE19. Oxidative stress was induced with <i>tert</i>-butyl hydroperoxide, cell viability evaluated with MTT assay, VEGF secretion assessed in ELISA. Phagocytosis was evaluated in a fluorescence microscopic assay. Wound healing ability was tested in a scratch assay. Additionally, the inhibition of elastase and complement system by Fe extract was studied. The Fe extract contained about 61.9% fucose and high amounts of uronic acids (26.2%). The sulfate content was not as high as expected (6.9%). It was not toxic and not protective against oxidative stress. However, Fe extract was able to reduce VEGF secretion in ARPE19. Phagocytosis was also reduced. Concerning wound healing, a delay could be observed in higher concentrations. While some beneficial effects could be found, it seems to interfere with RPE function, which may reduce its beneficial effects in AMD treatment.https://www.mdpi.com/1660-3397/17/9/538<i>Fucus distichus</i> subsp. <i>evanescens</i>fucoidanretinal pigment epitheliumVEGFoxidative stressphagocytosis |
| spellingShingle | Kevin Rohwer Sandesh Neupane Kaya Saskia Bittkau Mayra Galarza Pérez Philipp Dörschmann Johann Roider Susanne Alban Alexa Klettner Effects of Crude <i>Fucus distichus</i> Subspecies <i>evanescens</i> Fucoidan Extract on Retinal Pigment Epithelium Cells―Implications for Use in Age-Related Macular Degeneration Marine Drugs <i>Fucus distichus</i> subsp. <i>evanescens</i> fucoidan retinal pigment epithelium VEGF oxidative stress phagocytosis |
| title | Effects of Crude <i>Fucus distichus</i> Subspecies <i>evanescens</i> Fucoidan Extract on Retinal Pigment Epithelium Cells―Implications for Use in Age-Related Macular Degeneration |
| title_full | Effects of Crude <i>Fucus distichus</i> Subspecies <i>evanescens</i> Fucoidan Extract on Retinal Pigment Epithelium Cells―Implications for Use in Age-Related Macular Degeneration |
| title_fullStr | Effects of Crude <i>Fucus distichus</i> Subspecies <i>evanescens</i> Fucoidan Extract on Retinal Pigment Epithelium Cells―Implications for Use in Age-Related Macular Degeneration |
| title_full_unstemmed | Effects of Crude <i>Fucus distichus</i> Subspecies <i>evanescens</i> Fucoidan Extract on Retinal Pigment Epithelium Cells―Implications for Use in Age-Related Macular Degeneration |
| title_short | Effects of Crude <i>Fucus distichus</i> Subspecies <i>evanescens</i> Fucoidan Extract on Retinal Pigment Epithelium Cells―Implications for Use in Age-Related Macular Degeneration |
| title_sort | effects of crude i fucus distichus i subspecies i evanescens i fucoidan extract on retinal pigment epithelium cells implications for use in age related macular degeneration |
| topic | <i>Fucus distichus</i> subsp. <i>evanescens</i> fucoidan retinal pigment epithelium VEGF oxidative stress phagocytosis |
| url | https://www.mdpi.com/1660-3397/17/9/538 |
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