A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolites
Introduction: Ginger (Zingiber officinale Roscoe) can scavenge free radicals, which cause oxidative damage and inflamm-ageing. This study aimed to evaluate the antioxidant and anti-inflammatory effects of soil ginger's sub-critical water extracts (SWE) on different ages of Sprague Dawley (SD) r...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-02-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1006265/full |
| _version_ | 1811169459437568000 |
|---|---|
| author | Azraul Mumtazah Razak Azraul Mumtazah Razak Siti Nor Asyikin Zakaria Nur Fathiah Abdul Sani Nazirah Ab Rani Nur Haleeda Hakimi Mazlina Mohd Said Jen Kit Tan Han Kwang Gan Mariam Firdhaus Mad Nordin Suzana Makpol |
| author_facet | Azraul Mumtazah Razak Azraul Mumtazah Razak Siti Nor Asyikin Zakaria Nur Fathiah Abdul Sani Nazirah Ab Rani Nur Haleeda Hakimi Mazlina Mohd Said Jen Kit Tan Han Kwang Gan Mariam Firdhaus Mad Nordin Suzana Makpol |
| author_sort | Azraul Mumtazah Razak |
| collection | DOAJ |
| description | Introduction: Ginger (Zingiber officinale Roscoe) can scavenge free radicals, which cause oxidative damage and inflamm-ageing. This study aimed to evaluate the antioxidant and anti-inflammatory effects of soil ginger's sub-critical water extracts (SWE) on different ages of Sprague Dawley (SD) rats. The antioxidant properties and yield of SWE of soil- and soilless-grown ginger (soil ginger and soilless ginger will be used throughout the passage) were compared and evaluated.Methods: Three (young), nine (adult), and twenty-one (old) months old SD rats were subjected to oral gavage treatments with either distilled water or the SWE of soil ginger at a concentration of 200 mg/kg body weight (BW) for three months.Results: Soil ginger was found to yield 46% more extract than soilless ginger. While [6]-shogaol was more prevalent in soilless ginger, and [6]-gingerol concentration was higher in soil ginger (p < 0.05). Interestingly, soil ginger exhibited higher antioxidant activities than soilless ginger by using 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. With ginger treatment, a reduced levels of tumour necrosis factor-α (TNF-α) and C-reactive protein (CRP) but not interleukin-6 (IL-6) were observed in young rats. In all ages of SD rats, ginger treatment boosted catalase activity while lowering malondialdehyde (MDA). Reduction of urine 15-isoprostane F2t in young rats, creatine kinase-MM (CK-MM) in adult and old rats and lipid peroxidation (LPO) in young and adult rats were also observed.Discussion: The findings confirmed that the SWE of both soil and soilless grown ginger possessed antioxidant activities. Soil ginger produced a higher yield of extracts with a more prominent antioxidant activity. The SWE of soil ginger treatment on the different ages of SD rats ameliorates oxidative stress and inflammation responses. This could serve as the basis for developing a nutraceutical that can be used as a therapeutic intervention for ageing-related diseases. |
| first_indexed | 2024-04-10T16:42:45Z |
| format | Article |
| id | doaj.art-f1aa0f4c636a4da3ad49c0952e311eca |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-10T16:42:45Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-f1aa0f4c636a4da3ad49c0952e311eca2023-02-08T06:32:46ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-02-011410.3389/fphar.2023.10062651006265A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolitesAzraul Mumtazah Razak0Azraul Mumtazah Razak1Siti Nor Asyikin Zakaria2Nur Fathiah Abdul Sani3Nazirah Ab Rani4Nur Haleeda Hakimi5Mazlina Mohd Said6Jen Kit Tan7Han Kwang Gan8Mariam Firdhaus Mad Nordin9Suzana Makpol10Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalaysiaFaculty of Health Sciences, University College of MAIWP International, Kuala Lumpur, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalaysiaCentre of Drug and Herbal Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalaysiaMillercle Resources Sdn Bhd, Klang, MalaysiaAM Zaideen Ventures Sdn Bhd, Kuala Lumpur, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, MalaysiaIntroduction: Ginger (Zingiber officinale Roscoe) can scavenge free radicals, which cause oxidative damage and inflamm-ageing. This study aimed to evaluate the antioxidant and anti-inflammatory effects of soil ginger's sub-critical water extracts (SWE) on different ages of Sprague Dawley (SD) rats. The antioxidant properties and yield of SWE of soil- and soilless-grown ginger (soil ginger and soilless ginger will be used throughout the passage) were compared and evaluated.Methods: Three (young), nine (adult), and twenty-one (old) months old SD rats were subjected to oral gavage treatments with either distilled water or the SWE of soil ginger at a concentration of 200 mg/kg body weight (BW) for three months.Results: Soil ginger was found to yield 46% more extract than soilless ginger. While [6]-shogaol was more prevalent in soilless ginger, and [6]-gingerol concentration was higher in soil ginger (p < 0.05). Interestingly, soil ginger exhibited higher antioxidant activities than soilless ginger by using 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. With ginger treatment, a reduced levels of tumour necrosis factor-α (TNF-α) and C-reactive protein (CRP) but not interleukin-6 (IL-6) were observed in young rats. In all ages of SD rats, ginger treatment boosted catalase activity while lowering malondialdehyde (MDA). Reduction of urine 15-isoprostane F2t in young rats, creatine kinase-MM (CK-MM) in adult and old rats and lipid peroxidation (LPO) in young and adult rats were also observed.Discussion: The findings confirmed that the SWE of both soil and soilless grown ginger possessed antioxidant activities. Soil ginger produced a higher yield of extracts with a more prominent antioxidant activity. The SWE of soil ginger treatment on the different ages of SD rats ameliorates oxidative stress and inflammation responses. This could serve as the basis for developing a nutraceutical that can be used as a therapeutic intervention for ageing-related diseases.https://www.frontiersin.org/articles/10.3389/fphar.2023.1006265/fullZingiber officinale Roscoeantioxidantanti-inflammatoryanti-ageingsub-critical water extraction |
| spellingShingle | Azraul Mumtazah Razak Azraul Mumtazah Razak Siti Nor Asyikin Zakaria Nur Fathiah Abdul Sani Nazirah Ab Rani Nur Haleeda Hakimi Mazlina Mohd Said Jen Kit Tan Han Kwang Gan Mariam Firdhaus Mad Nordin Suzana Makpol A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolites Frontiers in Pharmacology Zingiber officinale Roscoe antioxidant anti-inflammatory anti-ageing sub-critical water extraction |
| title | A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolites |
| title_full | A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolites |
| title_fullStr | A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolites |
| title_full_unstemmed | A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolites |
| title_short | A subcritical water extract of soil grown Zingiber officinale Roscoe: Comparative analysis of antioxidant and anti-inflammatory effects and evaluation of bioactive metabolites |
| title_sort | subcritical water extract of soil grown zingiber officinale roscoe comparative analysis of antioxidant and anti inflammatory effects and evaluation of bioactive metabolites |
| topic | Zingiber officinale Roscoe antioxidant anti-inflammatory anti-ageing sub-critical water extraction |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1006265/full |
| work_keys_str_mv | AT azraulmumtazahrazak asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT azraulmumtazahrazak asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT sitinorasyikinzakaria asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT nurfathiahabdulsani asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT nazirahabrani asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT nurhaleedahakimi asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT mazlinamohdsaid asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT jenkittan asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT hankwanggan asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT mariamfirdhausmadnordin asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT suzanamakpol asubcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT azraulmumtazahrazak subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT azraulmumtazahrazak subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT sitinorasyikinzakaria subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT nurfathiahabdulsani subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT nazirahabrani subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT nurhaleedahakimi subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT mazlinamohdsaid subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT jenkittan subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT hankwanggan subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT mariamfirdhausmadnordin subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites AT suzanamakpol subcriticalwaterextractofsoilgrownzingiberofficinaleroscoecomparativeanalysisofantioxidantandantiinflammatoryeffectsandevaluationofbioactivemetabolites |