Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs
<p>Abstract</p> <p>An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performe...
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| Format: | Article |
| Language: | English |
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BMC
2011-02-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/8/1/79 |
| _version_ | 1818759335891697664 |
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| author | Kang Byunghak Choi YoungJin Park JoonSoo Cho SamHyun Kim Donguk Lee Changgyun Baek Kyoungah Song Jaehyoung Park Kwisung Choi Hwajung Cheon Doo-Sung |
| author_facet | Kang Byunghak Choi YoungJin Park JoonSoo Cho SamHyun Kim Donguk Lee Changgyun Baek Kyoungah Song Jaehyoung Park Kwisung Choi Hwajung Cheon Doo-Sung |
| author_sort | Kang Byunghak |
| collection | DOAJ |
| description | <p>Abstract</p> <p>An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it with a prototype strain (Noyce). At the nucleotide level, the P1 region (85.3%) had the highest identity value; at the amino acid level, the P3 region (98.0%) had the highest identity value. The two strains shared all cleavage sites, with the exception of the VP1/2A site, which was TY/GA in the Noyce strain but TR/GA in the Korean ECV 5 isolate. In Vero cells infected with the Korean ECV 5 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC<sub>50 </sub>value >100 μg/mL. Of the five drugs, only amantadine (IC<sub>50</sub>: 1 ± 0.42 μg/mL, TI: 100) and ribavirin (IC<sub>50</sub>: 22 ± 1.36 μg/mL, TI: 4.55) had any antiviral activity against the Korean ECV 5 isolate.</p> |
| first_indexed | 2024-12-18T06:41:06Z |
| format | Article |
| id | doaj.art-f1aa97236c2947d891b58302a1a62023 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-18T06:41:06Z |
| publishDate | 2011-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-f1aa97236c2947d891b58302a1a620232022-12-21T21:17:37ZengBMCVirology Journal1743-422X2011-02-01817910.1186/1743-422X-8-79Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugsKang ByunghakChoi YoungJinPark JoonSooCho SamHyunKim DongukLee ChanggyunBaek KyoungahSong JaehyoungPark KwisungChoi HwajungCheon Doo-Sung<p>Abstract</p> <p>An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it with a prototype strain (Noyce). At the nucleotide level, the P1 region (85.3%) had the highest identity value; at the amino acid level, the P3 region (98.0%) had the highest identity value. The two strains shared all cleavage sites, with the exception of the VP1/2A site, which was TY/GA in the Noyce strain but TR/GA in the Korean ECV 5 isolate. In Vero cells infected with the Korean ECV 5 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC<sub>50 </sub>value >100 μg/mL. Of the five drugs, only amantadine (IC<sub>50</sub>: 1 ± 0.42 μg/mL, TI: 100) and ribavirin (IC<sub>50</sub>: 22 ± 1.36 μg/mL, TI: 4.55) had any antiviral activity against the Korean ECV 5 isolate.</p>http://www.virologyj.com/content/8/1/79 |
| spellingShingle | Kang Byunghak Choi YoungJin Park JoonSoo Cho SamHyun Kim Donguk Lee Changgyun Baek Kyoungah Song Jaehyoung Park Kwisung Choi Hwajung Cheon Doo-Sung Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs Virology Journal |
| title | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
| title_full | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
| title_fullStr | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
| title_full_unstemmed | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
| title_short | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
| title_sort | genetic diversity of a korean echovirus 5 isolate and response of the strain to five antiviral drugs |
| url | http://www.virologyj.com/content/8/1/79 |
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