Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice
Inner mitochondrial membrane peptidase 2-like (IMMP2L) protein is a mitochondrial inner membrane peptidase that cleaves the signal peptide sequences of cytochrome c1 (CYC1) and mitochondrial glycerol phosphate dehydrogenase (GPD2). Immp2l mutant mice show infertility and early signs of aging. It is...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-01-01
|
| Series: | Redox Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231714000962 |
| _version_ | 1818485099676565504 |
|---|---|
| author | Manish S. Bharadwaj Yu Zhou Anthony J. Molina Tracy Criswell Baisong Lu |
| author_facet | Manish S. Bharadwaj Yu Zhou Anthony J. Molina Tracy Criswell Baisong Lu |
| author_sort | Manish S. Bharadwaj |
| collection | DOAJ |
| description | Inner mitochondrial membrane peptidase 2-like (IMMP2L) protein is a mitochondrial inner membrane peptidase that cleaves the signal peptide sequences of cytochrome c1 (CYC1) and mitochondrial glycerol phosphate dehydrogenase (GPD2). Immp2l mutant mice show infertility and early signs of aging. It is unclear whether mitochondrial respiratory deficiency underlies this phenotype. Here we show that the intermediate forms of GPD2 and CYC1 have normal expression levels and enzymatic function in Immp2l mutants. Mitochondrial respiration is not diminished in isolated mitochondria and cells from mutant mice. Our data suggest that respiratory deficiency is not the cause of the observed Immp2l mutant phenotypes. |
| first_indexed | 2024-12-10T16:03:50Z |
| format | Article |
| id | doaj.art-f1acfff1fdcf4aeb988370eb96768def |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-12-10T16:03:50Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-f1acfff1fdcf4aeb988370eb96768def2022-12-22T01:42:21ZengElsevierRedox Biology2213-23172014-01-012C1008101510.1016/j.redox.2014.08.006Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant miceManish S. Bharadwaj0Yu Zhou1Anthony J. Molina2Tracy Criswell3Baisong Lu4Section on Gerontology and Geriatric Medicine, Wake Forest University Health Sciences, Department of Internal Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USAWake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USASection on Gerontology and Geriatric Medicine, Wake Forest University Health Sciences, Department of Internal Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USAWake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USAWake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USAInner mitochondrial membrane peptidase 2-like (IMMP2L) protein is a mitochondrial inner membrane peptidase that cleaves the signal peptide sequences of cytochrome c1 (CYC1) and mitochondrial glycerol phosphate dehydrogenase (GPD2). Immp2l mutant mice show infertility and early signs of aging. It is unclear whether mitochondrial respiratory deficiency underlies this phenotype. Here we show that the intermediate forms of GPD2 and CYC1 have normal expression levels and enzymatic function in Immp2l mutants. Mitochondrial respiration is not diminished in isolated mitochondria and cells from mutant mice. Our data suggest that respiratory deficiency is not the cause of the observed Immp2l mutant phenotypes.http://www.sciencedirect.com/science/article/pii/S2213231714000962Immp2lCytochrome c1GPD2Complex IIIMitochondrial respiration |
| spellingShingle | Manish S. Bharadwaj Yu Zhou Anthony J. Molina Tracy Criswell Baisong Lu Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice Redox Biology Immp2l Cytochrome c1 GPD2 Complex III Mitochondrial respiration |
| title | Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice |
| title_full | Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice |
| title_fullStr | Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice |
| title_full_unstemmed | Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice |
| title_short | Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice |
| title_sort | examination of bioenergetic function in the inner mitochondrial membrane peptidase 2 like immp2l mutant mice |
| topic | Immp2l Cytochrome c1 GPD2 Complex III Mitochondrial respiration |
| url | http://www.sciencedirect.com/science/article/pii/S2213231714000962 |
| work_keys_str_mv | AT manishsbharadwaj examinationofbioenergeticfunctionintheinnermitochondrialmembranepeptidase2likeimmp2lmutantmice AT yuzhou examinationofbioenergeticfunctionintheinnermitochondrialmembranepeptidase2likeimmp2lmutantmice AT anthonyjmolina examinationofbioenergeticfunctionintheinnermitochondrialmembranepeptidase2likeimmp2lmutantmice AT tracycriswell examinationofbioenergeticfunctionintheinnermitochondrialmembranepeptidase2likeimmp2lmutantmice AT baisonglu examinationofbioenergeticfunctionintheinnermitochondrialmembranepeptidase2likeimmp2lmutantmice |