Benchmarking the proteomic profile of animal models of mesial temporal epilepsy
Abstract Objectives We compared the proteomic signatures of the hippocampal lesion induced in three different animal models of mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE+HS): the systemic pilocarpine model (PILO), the intracerebroventricular kainic acid model (KA), and the perfor...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-04-01
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| Series: | Annals of Clinical and Translational Neurology |
| Online Access: | https://doi.org/10.1002/acn3.51533 |
| _version_ | 1811272597281701888 |
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| author | Amanda M. Canto Alexandre B. Godoi Alexandre H. B. Matos Jaqueline C. Geraldis Fabio Rogerio Marina K. M. Alvim Clarissa L. Yasuda Enrico Ghizoni Helder Tedeschi Diogo F. T. Veiga Barbara Henning Welliton Souza Cristiane S. Rocha André S. Vieira Elayne V. Dias Benilton S. Carvalho Rovilson Gilioli Albert B. Arul Renã A. S. Robinson Fernando Cendes Iscia Lopes‐Cendes |
| author_facet | Amanda M. Canto Alexandre B. Godoi Alexandre H. B. Matos Jaqueline C. Geraldis Fabio Rogerio Marina K. M. Alvim Clarissa L. Yasuda Enrico Ghizoni Helder Tedeschi Diogo F. T. Veiga Barbara Henning Welliton Souza Cristiane S. Rocha André S. Vieira Elayne V. Dias Benilton S. Carvalho Rovilson Gilioli Albert B. Arul Renã A. S. Robinson Fernando Cendes Iscia Lopes‐Cendes |
| author_sort | Amanda M. Canto |
| collection | DOAJ |
| description | Abstract Objectives We compared the proteomic signatures of the hippocampal lesion induced in three different animal models of mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE+HS): the systemic pilocarpine model (PILO), the intracerebroventricular kainic acid model (KA), and the perforant pathway stimulation model (PPS). Methods We used shotgun proteomics to analyze the proteomes and find enriched biological pathways of the dorsal and ventral dentate gyrus (DG) isolated from the hippocampi of the three animal models. We also compared the proteomes obtained in the animal models to that from the DG of patients with pharmacoresistant MTLE+HS. Results We found that each animal model presents specific profiles of proteomic changes. The PILO model showed responses predominantly related to neuronal excitatory imbalance. The KA model revealed alterations mainly in synaptic activity. The PPS model displayed abnormalities in metabolism and oxidative stress. We also identified common biological pathways enriched in all three models, such as inflammation and immune response, which were also observed in tissue from patients. However, none of the models could recapitulate the profile of molecular changes observed in tissue from patients. Significance Our results indicate that each model has its own set of biological responses leading to epilepsy. Thus, it seems that only using a combination of the three models may one replicate more closely the mechanisms underlying MTLE+HS as seen in patients. |
| first_indexed | 2024-04-12T22:43:12Z |
| format | Article |
| id | doaj.art-f1b0e9ef2cfb478c82e204f073111d3e |
| institution | Directory Open Access Journal |
| issn | 2328-9503 |
| language | English |
| last_indexed | 2024-04-12T22:43:12Z |
| publishDate | 2022-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Annals of Clinical and Translational Neurology |
| spelling | doaj.art-f1b0e9ef2cfb478c82e204f073111d3e2022-12-22T03:13:40ZengWileyAnnals of Clinical and Translational Neurology2328-95032022-04-019445446710.1002/acn3.51533Benchmarking the proteomic profile of animal models of mesial temporal epilepsyAmanda M. Canto0Alexandre B. Godoi1Alexandre H. B. Matos2Jaqueline C. Geraldis3Fabio Rogerio4Marina K. M. Alvim5Clarissa L. Yasuda6Enrico Ghizoni7Helder Tedeschi8Diogo F. T. Veiga9Barbara Henning10Welliton Souza11Cristiane S. Rocha12André S. Vieira13Elayne V. Dias14Benilton S. Carvalho15Rovilson Gilioli16Albert B. Arul17Renã A. S. Robinson18Fernando Cendes19Iscia Lopes‐Cendes20Department of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilDepartment of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilDepartment of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilDepartment of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilBrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilBrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilBrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilBrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilBrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilDepartment of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilDepartment of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilDepartment of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilDepartment of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilBrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilBrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilBrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilLaboratory of Animal Quality Control University of Campinas (UNICAMP) Campinas SP BrazilDepartment of Chemistry Vanderbilt University Nashville Tennessee 37235 USADepartment of Chemistry Vanderbilt University Nashville Tennessee 37235 USABrazilian Institute of Neuroscience and Neurotechnology (BRAINN) Campinas SP BrazilDepartment of Translational Medicine, School of Medical Sciences University of Campinas (UNICAMP) Campinas SP BrazilAbstract Objectives We compared the proteomic signatures of the hippocampal lesion induced in three different animal models of mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE+HS): the systemic pilocarpine model (PILO), the intracerebroventricular kainic acid model (KA), and the perforant pathway stimulation model (PPS). Methods We used shotgun proteomics to analyze the proteomes and find enriched biological pathways of the dorsal and ventral dentate gyrus (DG) isolated from the hippocampi of the three animal models. We also compared the proteomes obtained in the animal models to that from the DG of patients with pharmacoresistant MTLE+HS. Results We found that each animal model presents specific profiles of proteomic changes. The PILO model showed responses predominantly related to neuronal excitatory imbalance. The KA model revealed alterations mainly in synaptic activity. The PPS model displayed abnormalities in metabolism and oxidative stress. We also identified common biological pathways enriched in all three models, such as inflammation and immune response, which were also observed in tissue from patients. However, none of the models could recapitulate the profile of molecular changes observed in tissue from patients. Significance Our results indicate that each model has its own set of biological responses leading to epilepsy. Thus, it seems that only using a combination of the three models may one replicate more closely the mechanisms underlying MTLE+HS as seen in patients.https://doi.org/10.1002/acn3.51533 |
| spellingShingle | Amanda M. Canto Alexandre B. Godoi Alexandre H. B. Matos Jaqueline C. Geraldis Fabio Rogerio Marina K. M. Alvim Clarissa L. Yasuda Enrico Ghizoni Helder Tedeschi Diogo F. T. Veiga Barbara Henning Welliton Souza Cristiane S. Rocha André S. Vieira Elayne V. Dias Benilton S. Carvalho Rovilson Gilioli Albert B. Arul Renã A. S. Robinson Fernando Cendes Iscia Lopes‐Cendes Benchmarking the proteomic profile of animal models of mesial temporal epilepsy Annals of Clinical and Translational Neurology |
| title | Benchmarking the proteomic profile of animal models of mesial temporal epilepsy |
| title_full | Benchmarking the proteomic profile of animal models of mesial temporal epilepsy |
| title_fullStr | Benchmarking the proteomic profile of animal models of mesial temporal epilepsy |
| title_full_unstemmed | Benchmarking the proteomic profile of animal models of mesial temporal epilepsy |
| title_short | Benchmarking the proteomic profile of animal models of mesial temporal epilepsy |
| title_sort | benchmarking the proteomic profile of animal models of mesial temporal epilepsy |
| url | https://doi.org/10.1002/acn3.51533 |
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