Multi-Omics Analysis Revealed the <i>AGR-FC.C3</i> Locus of <i>Brassica napus</i> as a Novel Candidate for Controlling Petal Color
Variations in the petal color of <i>Brassica napus</i> are crucial for ornamental value, but the controlled loci for breeding remain to be unraveled. Here, we report a candidate locus, <i>AGR-FC.C3</i>, having conducted a bulked segregant analysis on a segregating population...
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MDPI AG
2024-02-01
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| author | Yiran Ding Huaixin Li Xinmin Liu Xin Cheng Wang Chen Mingli Wu Liurong Chen Jianjie He Hongbo Chao Haibo Jia Chunhua Fu Maoteng Li |
| author_facet | Yiran Ding Huaixin Li Xinmin Liu Xin Cheng Wang Chen Mingli Wu Liurong Chen Jianjie He Hongbo Chao Haibo Jia Chunhua Fu Maoteng Li |
| author_sort | Yiran Ding |
| collection | DOAJ |
| description | Variations in the petal color of <i>Brassica napus</i> are crucial for ornamental value, but the controlled loci for breeding remain to be unraveled. Here, we report a candidate locus, <i>AGR-FC.C3</i>, having conducted a bulked segregant analysis on a segregating population with different petal colors. Our results showed that the locus covers 9.46 Mb of the genome, harboring 951 genes. BnaC03.MYB4, BnaC03.MYB85, BnaC03.MYB73, BnaC03.MYB98, and BnaC03.MYB102 belonging to MYB TFs families that might regulate the petal color were observed. Next, a bulk RNA sequencing of white and orange-yellow petals on three development stages was performed to further identify the possible governed genes. The results revealed a total of 51 genes by overlapping the transcriptome data and the bulked segregant analysis data, and it was found that the expression of <i>BnaC03.CCD4</i> was significantly up-regulated in the white petals at three development stages. Then, several novel candidate genes such as <i>BnaC03.ENDO3</i>, <i>BnaC03.T22F8.180</i>, <i>BnaC03.F15C21.8</i>, <i>BnaC03.Q8GSI6</i>, <i>BnaC03.LSD1</i>, <i>BnaC03.MAP1Da</i>, <i>BnaC03.MAP1Db</i>, and <i>BnaC03G0739700ZS</i> putative to controlling the petal color were identified through deeper analysis. Furthermo re, we have developed two molecular markers for the reported functional gene <i>BnaC03.CCD4</i> to discriminate the white and orange-yellow petal colors. Our results provided a novel locus for breeding rapeseed with multi-color petals. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-07T22:17:29Z |
| publishDate | 2024-02-01 |
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| series | Plants |
| spelling | doaj.art-f1b5a507ab604f018c27b29a4a127c482024-02-23T15:31:55ZengMDPI AGPlants2223-77472024-02-0113450710.3390/plants13040507Multi-Omics Analysis Revealed the <i>AGR-FC.C3</i> Locus of <i>Brassica napus</i> as a Novel Candidate for Controlling Petal ColorYiran Ding0Huaixin Li1Xinmin Liu2Xin Cheng3Wang Chen4Mingli Wu5Liurong Chen6Jianjie He7Hongbo Chao8Haibo Jia9Chunhua Fu10Maoteng Li11Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaSchool of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaVariations in the petal color of <i>Brassica napus</i> are crucial for ornamental value, but the controlled loci for breeding remain to be unraveled. Here, we report a candidate locus, <i>AGR-FC.C3</i>, having conducted a bulked segregant analysis on a segregating population with different petal colors. Our results showed that the locus covers 9.46 Mb of the genome, harboring 951 genes. BnaC03.MYB4, BnaC03.MYB85, BnaC03.MYB73, BnaC03.MYB98, and BnaC03.MYB102 belonging to MYB TFs families that might regulate the petal color were observed. Next, a bulk RNA sequencing of white and orange-yellow petals on three development stages was performed to further identify the possible governed genes. The results revealed a total of 51 genes by overlapping the transcriptome data and the bulked segregant analysis data, and it was found that the expression of <i>BnaC03.CCD4</i> was significantly up-regulated in the white petals at three development stages. Then, several novel candidate genes such as <i>BnaC03.ENDO3</i>, <i>BnaC03.T22F8.180</i>, <i>BnaC03.F15C21.8</i>, <i>BnaC03.Q8GSI6</i>, <i>BnaC03.LSD1</i>, <i>BnaC03.MAP1Da</i>, <i>BnaC03.MAP1Db</i>, and <i>BnaC03G0739700ZS</i> putative to controlling the petal color were identified through deeper analysis. Furthermo re, we have developed two molecular markers for the reported functional gene <i>BnaC03.CCD4</i> to discriminate the white and orange-yellow petal colors. Our results provided a novel locus for breeding rapeseed with multi-color petals.https://www.mdpi.com/2223-7747/13/4/507petal colorcarotenoidbulked segregant analysisdifferentially expressed genes |
| spellingShingle | Yiran Ding Huaixin Li Xinmin Liu Xin Cheng Wang Chen Mingli Wu Liurong Chen Jianjie He Hongbo Chao Haibo Jia Chunhua Fu Maoteng Li Multi-Omics Analysis Revealed the <i>AGR-FC.C3</i> Locus of <i>Brassica napus</i> as a Novel Candidate for Controlling Petal Color Plants petal color carotenoid bulked segregant analysis differentially expressed genes |
| title | Multi-Omics Analysis Revealed the <i>AGR-FC.C3</i> Locus of <i>Brassica napus</i> as a Novel Candidate for Controlling Petal Color |
| title_full | Multi-Omics Analysis Revealed the <i>AGR-FC.C3</i> Locus of <i>Brassica napus</i> as a Novel Candidate for Controlling Petal Color |
| title_fullStr | Multi-Omics Analysis Revealed the <i>AGR-FC.C3</i> Locus of <i>Brassica napus</i> as a Novel Candidate for Controlling Petal Color |
| title_full_unstemmed | Multi-Omics Analysis Revealed the <i>AGR-FC.C3</i> Locus of <i>Brassica napus</i> as a Novel Candidate for Controlling Petal Color |
| title_short | Multi-Omics Analysis Revealed the <i>AGR-FC.C3</i> Locus of <i>Brassica napus</i> as a Novel Candidate for Controlling Petal Color |
| title_sort | multi omics analysis revealed the i agr fc c3 i locus of i brassica napus i as a novel candidate for controlling petal color |
| topic | petal color carotenoid bulked segregant analysis differentially expressed genes |
| url | https://www.mdpi.com/2223-7747/13/4/507 |
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