Protocol for improving diffraction quality of leucyl-tRNA synthetase 1 with methylation and post-crystallization soaking and cooling in cryoprotectants

Protocol for improving diffraction quality of leucyl-tRNA synthetase 1 with methylation and post-crystallization soaking and cooling in cryoprotectants

Summary: Leucyl-tRNA synthetase 1 (LARS1) synthesizes Leu-tRNALeu for protein synthesis and plays an important role in mTORC1 activation by sensing intracellular leucine concentrations. Here, we describe a protocol for the purification, reductive methylation, binding affinity measurement by microsca...

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Bibliographic Details
Main Authors: Sulhee Kim, Ina Yoon, Sunghoon Kim, Kwang Yeon Hwang
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Protein Biochemistry
Structural Biology
X-ray Crystallography
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100349X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672100349X

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