Polyacrylate–Peptide Antigen Conjugate as a Single-Dose Oral Vaccine against Group A <i>Streptococcus</i>
Group A <i>Streptococcus</i> (GAS)-associated rheumatic heart disease is a leading cause of death caused by GAS infection. While antibiotics can treat the infection in most cases, growing antibiotic resistance, late medical intervention, and recurrent infection are major obstacles to the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/1/23 |
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| author | Mohammad Omer Faruck Lili Zhao Waleed M. Hussein Zeinab G. Khalil Robert J. Capon Mariusz Skwarczynski Istvan Toth |
| author_facet | Mohammad Omer Faruck Lili Zhao Waleed M. Hussein Zeinab G. Khalil Robert J. Capon Mariusz Skwarczynski Istvan Toth |
| author_sort | Mohammad Omer Faruck |
| collection | DOAJ |
| description | Group A <i>Streptococcus</i> (GAS)-associated rheumatic heart disease is a leading cause of death caused by GAS infection. While antibiotics can treat the infection in most cases, growing antibiotic resistance, late medical intervention, and recurrent infection are major obstacles to the effective treatment of GAS-associated diseases. As GAS infection typically originates from the bacterial colonization of mucosal tissue in the throat, an oral vaccine that can generate both systemic and mucosal immune responses would solve problems associated with traditional medical interventions. Moreover, orally delivered vaccines are more easily administered and less expensive for mass immunization. In this study, the B-cell epitope J8, derived from GAS M protein, and universal T-helper Pan HLA-DR-binding epitope peptide (PADRE), were conjugated to poly (methyl acrylate) (PMA) to form a self-assembled nanoparticle vaccine candidate (PMA-P-J8). Strong systemic and mucosal immune responses were induced upon single oral immunization of mice with the conjugate. The antibodies generated were opsonic against GAS clinical isolates as measured after boost immunization. Thus, we developed a simple conjugate as an effective, adjuvant-free oral peptide-based vaccine. |
| first_indexed | 2024-04-12T19:30:17Z |
| format | Article |
| id | doaj.art-f1b76c83d3d540dfb4de265e12d40fb9 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-12T19:30:17Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-f1b76c83d3d540dfb4de265e12d40fb92022-12-22T03:19:23ZengMDPI AGVaccines2076-393X2020-01-01812310.3390/vaccines8010023vaccines8010023Polyacrylate–Peptide Antigen Conjugate as a Single-Dose Oral Vaccine against Group A <i>Streptococcus</i>Mohammad Omer Faruck0Lili Zhao1Waleed M. Hussein2Zeinab G. Khalil3Robert J. Capon4Mariusz Skwarczynski5Istvan Toth6School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, QLD 4072, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, QLD 4072, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, QLD 4072, AustraliaInstitute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, AustraliaInstitute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, QLD 4072, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, QLD 4072, AustraliaGroup A <i>Streptococcus</i> (GAS)-associated rheumatic heart disease is a leading cause of death caused by GAS infection. While antibiotics can treat the infection in most cases, growing antibiotic resistance, late medical intervention, and recurrent infection are major obstacles to the effective treatment of GAS-associated diseases. As GAS infection typically originates from the bacterial colonization of mucosal tissue in the throat, an oral vaccine that can generate both systemic and mucosal immune responses would solve problems associated with traditional medical interventions. Moreover, orally delivered vaccines are more easily administered and less expensive for mass immunization. In this study, the B-cell epitope J8, derived from GAS M protein, and universal T-helper Pan HLA-DR-binding epitope peptide (PADRE), were conjugated to poly (methyl acrylate) (PMA) to form a self-assembled nanoparticle vaccine candidate (PMA-P-J8). Strong systemic and mucosal immune responses were induced upon single oral immunization of mice with the conjugate. The antibodies generated were opsonic against GAS clinical isolates as measured after boost immunization. Thus, we developed a simple conjugate as an effective, adjuvant-free oral peptide-based vaccine.https://www.mdpi.com/2076-393X/8/1/23peptide vaccinepoly (methyl acrylate)oral deliverynanoparticlespolymer–peptide conjugategroup a <i>streptococcus</i> |
| spellingShingle | Mohammad Omer Faruck Lili Zhao Waleed M. Hussein Zeinab G. Khalil Robert J. Capon Mariusz Skwarczynski Istvan Toth Polyacrylate–Peptide Antigen Conjugate as a Single-Dose Oral Vaccine against Group A <i>Streptococcus</i> Vaccines peptide vaccine poly (methyl acrylate) oral delivery nanoparticles polymer–peptide conjugate group a <i>streptococcus</i> |
| title | Polyacrylate–Peptide Antigen Conjugate as a Single-Dose Oral Vaccine against Group A <i>Streptococcus</i> |
| title_full | Polyacrylate–Peptide Antigen Conjugate as a Single-Dose Oral Vaccine against Group A <i>Streptococcus</i> |
| title_fullStr | Polyacrylate–Peptide Antigen Conjugate as a Single-Dose Oral Vaccine against Group A <i>Streptococcus</i> |
| title_full_unstemmed | Polyacrylate–Peptide Antigen Conjugate as a Single-Dose Oral Vaccine against Group A <i>Streptococcus</i> |
| title_short | Polyacrylate–Peptide Antigen Conjugate as a Single-Dose Oral Vaccine against Group A <i>Streptococcus</i> |
| title_sort | polyacrylate peptide antigen conjugate as a single dose oral vaccine against group a i streptococcus i |
| topic | peptide vaccine poly (methyl acrylate) oral delivery nanoparticles polymer–peptide conjugate group a <i>streptococcus</i> |
| url | https://www.mdpi.com/2076-393X/8/1/23 |
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