Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics

All the time, echinococcosis is a global zoonotic disease which seriously endangers public health all over the world. In order to speed up the development process of anti-Echinococcus granulosus vaccine, at the same time, it can also save economic cost. In this study, immunoinformatics tools and mol...

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Main Authors: Mingkai Yu, Yuejie Zhu, Yujiao Li, Zhiqiang Chen, Tong Sha, Zhiwei Li, Fengbo Zhang, Jianbing Ding
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-08-01
Series:Frontiers in Immunology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2021.668492/full
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author Mingkai Yu
Yuejie Zhu
Yujiao Li
Zhiqiang Chen
Tong Sha
Zhiwei Li
Fengbo Zhang
Fengbo Zhang
Jianbing Ding
Jianbing Ding
author_facet Mingkai Yu
Yuejie Zhu
Yujiao Li
Zhiqiang Chen
Tong Sha
Zhiwei Li
Fengbo Zhang
Fengbo Zhang
Jianbing Ding
Jianbing Ding
author_sort Mingkai Yu
collection DOAJ
description All the time, echinococcosis is a global zoonotic disease which seriously endangers public health all over the world. In order to speed up the development process of anti-Echinococcus granulosus vaccine, at the same time, it can also save economic cost. In this study, immunoinformatics tools and molecular docking methods were used to predict and screen the antigen epitopes of Echinococcus granulosus, to design a multi-epitope vaccine containing B- and T-cell epitopes. The multi-epitope vaccine could activate B lymphocytes to produce specific antibodies theoretically, which could protect the human body against Echinococcus granulosus infection. It also could activate T lymphocytes and clear the infected parasites in the body. In this study, four CD8+ T-cell epitopes, three CD4+ T-cell epitopes and four B-cell epitopes of Protein EgTeg were identified by immunoinformatics methods. Meanwhile, three CD8+ T-cell epitopes, two CD4+ T-cell epitopes and four B-cell epitopes of Protein EgFABP1 were identified. We constructed the multi-epitope vaccine using linker proteins. The study based on the traditional methods of antigen epitope prediction, further optimized the prediction results combined with molecular docking technology and improved the precision and accuracy of the results. Finally, in vivo and in vitro experiments had verified that the vaccine designed in this study had good antigenicity and immunogenicity.
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spelling doaj.art-f1b795ba27484aa18fe142de4045bdc52022-12-21T18:27:37ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-08-011210.3389/fimmu.2021.668492668492Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in ImmunoinformaticsMingkai Yu0Yuejie Zhu1Yujiao Li2Zhiqiang Chen3Tong Sha4Zhiwei Li5Fengbo Zhang6Fengbo Zhang7Jianbing Ding8Jianbing Ding9Department of Immunology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, ChinaReproductive Medicine Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaDepartment of Blood Transfusion, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaDepartment of Immunology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, ChinaDepartment of Immunology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, ChinaClinical Laboratory Center, Xinjiang Uygur Autonomous Region People's Hospital, Urumqi, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, Treatment of Central Asian High Incidence Diseases, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaDepartment of Immunology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, Treatment of Central Asian High Incidence Diseases, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaAll the time, echinococcosis is a global zoonotic disease which seriously endangers public health all over the world. In order to speed up the development process of anti-Echinococcus granulosus vaccine, at the same time, it can also save economic cost. In this study, immunoinformatics tools and molecular docking methods were used to predict and screen the antigen epitopes of Echinococcus granulosus, to design a multi-epitope vaccine containing B- and T-cell epitopes. The multi-epitope vaccine could activate B lymphocytes to produce specific antibodies theoretically, which could protect the human body against Echinococcus granulosus infection. It also could activate T lymphocytes and clear the infected parasites in the body. In this study, four CD8+ T-cell epitopes, three CD4+ T-cell epitopes and four B-cell epitopes of Protein EgTeg were identified by immunoinformatics methods. Meanwhile, three CD8+ T-cell epitopes, two CD4+ T-cell epitopes and four B-cell epitopes of Protein EgFABP1 were identified. We constructed the multi-epitope vaccine using linker proteins. The study based on the traditional methods of antigen epitope prediction, further optimized the prediction results combined with molecular docking technology and improved the precision and accuracy of the results. Finally, in vivo and in vitro experiments had verified that the vaccine designed in this study had good antigenicity and immunogenicity.https://www.frontiersin.org/articles/10.3389/fimmu.2021.668492/fullimmunoinformaticsmulti-epitopeEchinococcus granulosusmolecular dockingvaccine
spellingShingle Mingkai Yu
Yuejie Zhu
Yujiao Li
Zhiqiang Chen
Tong Sha
Zhiwei Li
Fengbo Zhang
Fengbo Zhang
Jianbing Ding
Jianbing Ding
Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics
Frontiers in Immunology
immunoinformatics
multi-epitope
Echinococcus granulosus
molecular docking
vaccine
title Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics
title_full Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics
title_fullStr Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics
title_full_unstemmed Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics
title_short Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics
title_sort design of a novel multi epitope vaccine against echinococcus granulosus in immunoinformatics
topic immunoinformatics
multi-epitope
Echinococcus granulosus
molecular docking
vaccine
url https://www.frontiersin.org/articles/10.3389/fimmu.2021.668492/full
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