Effect of leptin infusion on insulin sensitivity and lipid metabolism in diet-induced lipodystrophy model mice
<p>Abstract</p> <p>Background</p> <p>Lipodystrophies are rare acquired and genetic disorders characterized by the complete or partial absence of body fat with a line of metabolic disorders. Previous studies demonstrated that dietary conjugated linoleic acid (CLA) induce...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-03-01
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| Series: | Lipids in Health and Disease |
| Online Access: | http://www.lipidworld.com/content/7/1/8 |
| _version_ | 1818755652188635136 |
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| author | Shirouchi Bungo Higa Kouki Ujino Yoko Inoue Nao Nagao Koji Wang Yu-Ming Yanagita Teruyoshi |
| author_facet | Shirouchi Bungo Higa Kouki Ujino Yoko Inoue Nao Nagao Koji Wang Yu-Ming Yanagita Teruyoshi |
| author_sort | Shirouchi Bungo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Lipodystrophies are rare acquired and genetic disorders characterized by the complete or partial absence of body fat with a line of metabolic disorders. Previous studies demonstrated that dietary conjugated linoleic acid (CLA) induces hepatic steatosis and hyperinsulinemia through the drastic reduction of adipocytokine levels due to a paucity of adipose tissue in mice and the pathogenesis of these metabolic abnormalities in CLA-fed mice is similar to that in human lipodystrophy. The present study explores the effect of leptin infusion on the pathogenesis of diet-induced lipodystrophy in mice. C57BL/6N mice were assigned to three groups: (1) mice were fed a semisynthetic diet supplemented with 6% corn oil and infused PBS intraperitoneally (normal group), (2) mice were fed a semisynthetic diet supplemented with 4% corn oil plus 2% CLA and infused PBS intraperitoneally (lipodystrophy-control group), and (3) mice were fed a semisynthetic diet supplemented with 4% corn oil plus 2% CLA and infused recombinant murine leptin intraperitoneally (lipodystrophy-leptin group). All mice were fed normal or lipodystrophy model diets for 4 weeks and were infused intrapeneally 0 or 5 <it>μ</it>g of leptin per day from third week of the feeding period for 1 week.</p> <p>Results</p> <p>The results indicate that leptin infusion can attenuate hepatic steatosis and hyperinsulinemia through the reduction of hepatic triglyceride synthesis and the improvement of insulin sensitivity in diet-induced lipodystrophy model mice.</p> <p>Conclusion</p> <p>We expect the use of this model for clarifying the pathophysiology of lipodystrophy-induced metabolic abnormalities and evaluating the efficacy and safety of drug and dietary treatment.</p> |
| first_indexed | 2024-12-18T05:42:33Z |
| format | Article |
| id | doaj.art-f1b7cc26c84949c6a0b4b8ecc98aa3b9 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-18T05:42:33Z |
| publishDate | 2008-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-f1b7cc26c84949c6a0b4b8ecc98aa3b92022-12-21T21:19:09ZengBMCLipids in Health and Disease1476-511X2008-03-0171810.1186/1476-511X-7-8Effect of leptin infusion on insulin sensitivity and lipid metabolism in diet-induced lipodystrophy model miceShirouchi BungoHiga KoukiUjino YokoInoue NaoNagao KojiWang Yu-MingYanagita Teruyoshi<p>Abstract</p> <p>Background</p> <p>Lipodystrophies are rare acquired and genetic disorders characterized by the complete or partial absence of body fat with a line of metabolic disorders. Previous studies demonstrated that dietary conjugated linoleic acid (CLA) induces hepatic steatosis and hyperinsulinemia through the drastic reduction of adipocytokine levels due to a paucity of adipose tissue in mice and the pathogenesis of these metabolic abnormalities in CLA-fed mice is similar to that in human lipodystrophy. The present study explores the effect of leptin infusion on the pathogenesis of diet-induced lipodystrophy in mice. C57BL/6N mice were assigned to three groups: (1) mice were fed a semisynthetic diet supplemented with 6% corn oil and infused PBS intraperitoneally (normal group), (2) mice were fed a semisynthetic diet supplemented with 4% corn oil plus 2% CLA and infused PBS intraperitoneally (lipodystrophy-control group), and (3) mice were fed a semisynthetic diet supplemented with 4% corn oil plus 2% CLA and infused recombinant murine leptin intraperitoneally (lipodystrophy-leptin group). All mice were fed normal or lipodystrophy model diets for 4 weeks and were infused intrapeneally 0 or 5 <it>μ</it>g of leptin per day from third week of the feeding period for 1 week.</p> <p>Results</p> <p>The results indicate that leptin infusion can attenuate hepatic steatosis and hyperinsulinemia through the reduction of hepatic triglyceride synthesis and the improvement of insulin sensitivity in diet-induced lipodystrophy model mice.</p> <p>Conclusion</p> <p>We expect the use of this model for clarifying the pathophysiology of lipodystrophy-induced metabolic abnormalities and evaluating the efficacy and safety of drug and dietary treatment.</p>http://www.lipidworld.com/content/7/1/8 |
| spellingShingle | Shirouchi Bungo Higa Kouki Ujino Yoko Inoue Nao Nagao Koji Wang Yu-Ming Yanagita Teruyoshi Effect of leptin infusion on insulin sensitivity and lipid metabolism in diet-induced lipodystrophy model mice Lipids in Health and Disease |
| title | Effect of leptin infusion on insulin sensitivity and lipid metabolism in diet-induced lipodystrophy model mice |
| title_full | Effect of leptin infusion on insulin sensitivity and lipid metabolism in diet-induced lipodystrophy model mice |
| title_fullStr | Effect of leptin infusion on insulin sensitivity and lipid metabolism in diet-induced lipodystrophy model mice |
| title_full_unstemmed | Effect of leptin infusion on insulin sensitivity and lipid metabolism in diet-induced lipodystrophy model mice |
| title_short | Effect of leptin infusion on insulin sensitivity and lipid metabolism in diet-induced lipodystrophy model mice |
| title_sort | effect of leptin infusion on insulin sensitivity and lipid metabolism in diet induced lipodystrophy model mice |
| url | http://www.lipidworld.com/content/7/1/8 |
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