Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone
Kaempferia parviflora Wall. ex Baker is a medicinal plant found in the upper Northeastern regions of Thailand, which belongs to Zingiberaceae family. The present study aims to investigate the standardization parameters, to analyze chemical constituents of volatile oil by gas chromatography-mass spec...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
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| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2018;volume=9;issue=1;spage=26;epage=31;aulast=Pitakpawasutthi |
| _version_ | 1828778604882821120 |
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| author | Yamon Pitakpawasutthi Chanida Palanuvej Nijsiri Ruangrungsi |
| author_facet | Yamon Pitakpawasutthi Chanida Palanuvej Nijsiri Ruangrungsi |
| author_sort | Yamon Pitakpawasutthi |
| collection | DOAJ |
| description | Kaempferia parviflora Wall. ex Baker is a medicinal plant found in the upper Northeastern regions of Thailand, which belongs to Zingiberaceae family. The present study aims to investigate the standardization parameters, to analyze chemical constituents of volatile oil by gas chromatography-mass spectrometry, and to determine the content of 5,7-dimethoxyflavone in K. parviflora rhizomes by thin-layer chromatography (TLC)-densitometry compared to TLC image analysis. K. parviflora rhizomes from 15 different sources throughout Thailand were investigated for morphological and pharmacognostic parameters. 5,7-Dimethoxyflavone contents were determined by TLC-densitometry with winCATS software and TLC image analysis with ImageJ software. The mobile phase for TLC development consisted of toluene: chloroform: Acetone: formic acid (5: 4: 1: 0.2). For the Results, the pharmacognostic parameters of K. parviflora rhizome were demonstrated. The loss on drying, total ash, acid-insoluble ash, water content, volatile oil content, ethanol, and water-soluble extractive values were found to be 8.979 ± 0.041, 5.127 ± 0.060, 2.174 ± 0.092, 9.291 ± 0.458, 0.028 ± 0.003, 5.138 ± 0.092, and 8.254 ± 0.191 g/100 g of dry weight, respectively. K. parviflora volatile oil showed the major components of α-copaene, dauca-5, 8-diene, camphene, β-pinene, borneol, and linalool. The 5,7-dimethoxyflavone content of K. parviflora rhizomes determined by TLC-densitometry and TLC image analysis were found to be 2.15 ± 0.64 and 1.96 ± 0.51 g/100 g of dry rhizomes, respectively. The 5,7-dimethoxyflavone contents of both methods were not significantly different (P > 0.05) using paired t-test. |
| first_indexed | 2024-12-11T16:45:39Z |
| format | Article |
| id | doaj.art-f1b7db76942148e98f36361b2fc785e2 |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-12-11T16:45:39Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-f1b7db76942148e98f36361b2fc785e22022-12-22T00:58:14ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942018-01-0191263110.4103/japtr.JAPTR_147_17Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavoneYamon PitakpawasutthiChanida PalanuvejNijsiri RuangrungsiKaempferia parviflora Wall. ex Baker is a medicinal plant found in the upper Northeastern regions of Thailand, which belongs to Zingiberaceae family. The present study aims to investigate the standardization parameters, to analyze chemical constituents of volatile oil by gas chromatography-mass spectrometry, and to determine the content of 5,7-dimethoxyflavone in K. parviflora rhizomes by thin-layer chromatography (TLC)-densitometry compared to TLC image analysis. K. parviflora rhizomes from 15 different sources throughout Thailand were investigated for morphological and pharmacognostic parameters. 5,7-Dimethoxyflavone contents were determined by TLC-densitometry with winCATS software and TLC image analysis with ImageJ software. The mobile phase for TLC development consisted of toluene: chloroform: Acetone: formic acid (5: 4: 1: 0.2). For the Results, the pharmacognostic parameters of K. parviflora rhizome were demonstrated. The loss on drying, total ash, acid-insoluble ash, water content, volatile oil content, ethanol, and water-soluble extractive values were found to be 8.979 ± 0.041, 5.127 ± 0.060, 2.174 ± 0.092, 9.291 ± 0.458, 0.028 ± 0.003, 5.138 ± 0.092, and 8.254 ± 0.191 g/100 g of dry weight, respectively. K. parviflora volatile oil showed the major components of α-copaene, dauca-5, 8-diene, camphene, β-pinene, borneol, and linalool. The 5,7-dimethoxyflavone content of K. parviflora rhizomes determined by TLC-densitometry and TLC image analysis were found to be 2.15 ± 0.64 and 1.96 ± 0.51 g/100 g of dry rhizomes, respectively. The 5,7-dimethoxyflavone contents of both methods were not significantly different (P > 0.05) using paired t-test.http://www.japtr.org/article.asp?issn=2231-4040;year=2018;volume=9;issue=1;spage=26;epage=31;aulast=Pitakpawasutthi57-dimethoxyflavoneKaempferia parviflora phamacognostic specificationquantitative analysisthin-layer chromatography image analysisthin-layer chromatography-densitometry |
| spellingShingle | Yamon Pitakpawasutthi Chanida Palanuvej Nijsiri Ruangrungsi Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone Journal of Advanced Pharmaceutical Technology & Research 5 7-dimethoxyflavone Kaempferia parviflora phamacognostic specification quantitative analysis thin-layer chromatography image analysis thin-layer chromatography-densitometry |
| title | Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone |
| title_full | Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone |
| title_fullStr | Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone |
| title_full_unstemmed | Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone |
| title_short | Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone |
| title_sort | quality evaluation of kaempferia parviflora rhizome with reference to 5 7 dimethoxyflavone |
| topic | 5 7-dimethoxyflavone Kaempferia parviflora phamacognostic specification quantitative analysis thin-layer chromatography image analysis thin-layer chromatography-densitometry |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2018;volume=9;issue=1;spage=26;epage=31;aulast=Pitakpawasutthi |
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