An age-wise comparison of human airway smooth muscle proliferative capacity.
We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4370680?pdf=render |
| _version_ | 1818153889163116544 |
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| author | Michael Fayon Annick Andrieux Imane Bara Muriel Rebola André Labbé Roger Marthan Patrick Berger |
| author_facet | Michael Fayon Annick Andrieux Imane Bara Muriel Rebola André Labbé Roger Marthan Patrick Berger |
| author_sort | Michael Fayon |
| collection | DOAJ |
| description | We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and PDGF-AA. In the presence of glucose, cell counts were significantly greater in neonatal vs. adult ASMC. Similarly, neonatal ASMC DNA synthesis in 10% FCS and PDGF-AA, and [Ca2+]i responses in the presence of histamine were significantly enhanced vs. adults. In glucose-free medium, cell proliferation was preserved in neonatal cells, unlike in adult cells, with concomitant increased porin (an indicator of mitochondrial activity) protein expression. Compared to adults, stimulated neonatal human ASMC are in a rapid and robust proliferative phase and have the capacity to respond disproportionately under abnormal environmental conditions, through increased mitochondrial biogenesis and altered calcium homeostasis. |
| first_indexed | 2024-12-11T14:17:47Z |
| format | Article |
| id | doaj.art-f1b7e2d4b8a4407aa5ee6b8afc5c5041 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T14:17:47Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f1b7e2d4b8a4407aa5ee6b8afc5c50412022-12-22T01:03:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012244610.1371/journal.pone.0122446An age-wise comparison of human airway smooth muscle proliferative capacity.Michael FayonAnnick AndrieuxImane BaraMuriel RebolaAndré LabbéRoger MarthanPatrick BergerWe compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and PDGF-AA. In the presence of glucose, cell counts were significantly greater in neonatal vs. adult ASMC. Similarly, neonatal ASMC DNA synthesis in 10% FCS and PDGF-AA, and [Ca2+]i responses in the presence of histamine were significantly enhanced vs. adults. In glucose-free medium, cell proliferation was preserved in neonatal cells, unlike in adult cells, with concomitant increased porin (an indicator of mitochondrial activity) protein expression. Compared to adults, stimulated neonatal human ASMC are in a rapid and robust proliferative phase and have the capacity to respond disproportionately under abnormal environmental conditions, through increased mitochondrial biogenesis and altered calcium homeostasis.http://europepmc.org/articles/PMC4370680?pdf=render |
| spellingShingle | Michael Fayon Annick Andrieux Imane Bara Muriel Rebola André Labbé Roger Marthan Patrick Berger An age-wise comparison of human airway smooth muscle proliferative capacity. PLoS ONE |
| title | An age-wise comparison of human airway smooth muscle proliferative capacity. |
| title_full | An age-wise comparison of human airway smooth muscle proliferative capacity. |
| title_fullStr | An age-wise comparison of human airway smooth muscle proliferative capacity. |
| title_full_unstemmed | An age-wise comparison of human airway smooth muscle proliferative capacity. |
| title_short | An age-wise comparison of human airway smooth muscle proliferative capacity. |
| title_sort | age wise comparison of human airway smooth muscle proliferative capacity |
| url | http://europepmc.org/articles/PMC4370680?pdf=render |
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