Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats
<em><strong>Objective(s):</strong></em> Noise-induced hearing loss is one of the most common occupational diseases in industrialized countries and can be affected by various environmental and genetic factors. This study was designed to examine the effect of myricetin in preve...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_14789_5dee104a41771ca9a7a5b0c6173ec646.pdf |
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| author | Maryam Bahaloo Mohammad Ebrahim Rezvani Ehsan Farashahi Yazd Fatemeh Zare Mehrjerdi Mohammad Hossein Davari Ali Roohbakhsh Abolfazl Mollasadeghi Haniyeh Nikkhah Maryam Vafaei Amir Houshang Mehrparvar |
| author_facet | Maryam Bahaloo Mohammad Ebrahim Rezvani Ehsan Farashahi Yazd Fatemeh Zare Mehrjerdi Mohammad Hossein Davari Ali Roohbakhsh Abolfazl Mollasadeghi Haniyeh Nikkhah Maryam Vafaei Amir Houshang Mehrparvar |
| author_sort | Maryam Bahaloo |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> Noise-induced hearing loss is one of the most common occupational diseases in industrialized countries and can be affected by various environmental and genetic factors. This study was designed to examine the effect of myricetin in preventing this disorder.<br /><em><strong>Materials and Methods:</strong></em> Twenty-one Wistar rats were randomly divided into five groups: Non-exposed, noise exposure only, noise exposure with vehicle, noise exposure with myricetin 5 mg/Kg, and noise exposure with myricetin 10 mg/kg. All animals were sacrificed after last noise exposure. The left cochlea was dissected from each rat. It was used for mRNA expression analysis (NOX3, TGF-β1, prestin, and HSP-70). Blood samples were collected to assess superoxide dismutase (SOD) activity, 1, 1 diphenyl picrylhydrazyl (DPPH), and malondialdehyde (MDA) measurements.<br /><em><strong>Results:</strong></em> Real time-PCR assay revealed that noise decreased NOX3 and increased TGF-β1, prestin, and HSP-70 gene expressions. Administration of myricetin at the dose of 5 mg/kg, but not at 10 mg/kg, significantly reversed these changes. Noise also increased MDA levels and decreased SOD and DPPH scavenging activities. Myricetin at the doses of 5 and 10 mg/kg also reversed these changes. <br /><em><strong>Conclusion:</strong></em> The findings of this study showed that myricetin at the dose of 5 mg/Kg was able to reverse noise-induced abnormalities in gene expression and oxidant/anti-oxidant balance. It is a possibility that myricetin via enhancement of anti-oxidant activity induced these effects. |
| first_indexed | 2024-12-10T14:19:35Z |
| format | Article |
| id | doaj.art-f1b9ca6e89a4402593f26c8e7baeb860 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-10T14:19:35Z |
| publishDate | 2020-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-f1b9ca6e89a4402593f26c8e7baeb8602022-12-22T01:45:14ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-05-0123559459910.22038/ijbms.2020.41007.969314789Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed ratsMaryam Bahaloo0Mohammad Ebrahim Rezvani1Ehsan Farashahi Yazd2Fatemeh Zare Mehrjerdi3Mohammad Hossein Davari4Ali Roohbakhsh5Abolfazl Mollasadeghi6Haniyeh Nikkhah7Maryam Vafaei8Amir Houshang Mehrparvar9Industrial Diseases Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, IranStem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Occupational Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranPharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranIndustrial Diseases Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranStem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranStem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranIndustrial Diseases Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran<em><strong>Objective(s):</strong></em> Noise-induced hearing loss is one of the most common occupational diseases in industrialized countries and can be affected by various environmental and genetic factors. This study was designed to examine the effect of myricetin in preventing this disorder.<br /><em><strong>Materials and Methods:</strong></em> Twenty-one Wistar rats were randomly divided into five groups: Non-exposed, noise exposure only, noise exposure with vehicle, noise exposure with myricetin 5 mg/Kg, and noise exposure with myricetin 10 mg/kg. All animals were sacrificed after last noise exposure. The left cochlea was dissected from each rat. It was used for mRNA expression analysis (NOX3, TGF-β1, prestin, and HSP-70). Blood samples were collected to assess superoxide dismutase (SOD) activity, 1, 1 diphenyl picrylhydrazyl (DPPH), and malondialdehyde (MDA) measurements.<br /><em><strong>Results:</strong></em> Real time-PCR assay revealed that noise decreased NOX3 and increased TGF-β1, prestin, and HSP-70 gene expressions. Administration of myricetin at the dose of 5 mg/kg, but not at 10 mg/kg, significantly reversed these changes. Noise also increased MDA levels and decreased SOD and DPPH scavenging activities. Myricetin at the doses of 5 and 10 mg/kg also reversed these changes. <br /><em><strong>Conclusion:</strong></em> The findings of this study showed that myricetin at the dose of 5 mg/Kg was able to reverse noise-induced abnormalities in gene expression and oxidant/anti-oxidant balance. It is a possibility that myricetin via enhancement of anti-oxidant activity induced these effects.http://ijbms.mums.ac.ir/article_14789_5dee104a41771ca9a7a5b0c6173ec646.pdfanti-oxidantsgene expressionmyricetinnadph oxidasenoise-induced hearing losssuperoxide dismutase |
| spellingShingle | Maryam Bahaloo Mohammad Ebrahim Rezvani Ehsan Farashahi Yazd Fatemeh Zare Mehrjerdi Mohammad Hossein Davari Ali Roohbakhsh Abolfazl Mollasadeghi Haniyeh Nikkhah Maryam Vafaei Amir Houshang Mehrparvar Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats Iranian Journal of Basic Medical Sciences anti-oxidants gene expression myricetin nadph oxidase noise-induced hearing loss superoxide dismutase |
| title | Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats |
| title_full | Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats |
| title_fullStr | Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats |
| title_full_unstemmed | Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats |
| title_short | Effect of myricetin on the gene expressions of NOX3, TGF-β1, prestin, and HSP-70 and anti-oxidant activity in the cochlea of noise-exposed rats |
| title_sort | effect of myricetin on the gene expressions of nox3 tgf β1 prestin and hsp 70 and anti oxidant activity in the cochlea of noise exposed rats |
| topic | anti-oxidants gene expression myricetin nadph oxidase noise-induced hearing loss superoxide dismutase |
| url | http://ijbms.mums.ac.ir/article_14789_5dee104a41771ca9a7a5b0c6173ec646.pdf |
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