RNA Editing at the Q/R Site for the Glutamate Receptor Subunits GLUR2, GLUR5, and GLUR6 in Hippocampus and Temporal Cortex from Epileptic Patients
Posttranscriptional editing of mRNA is a phenomenon that generates molecular heterogeneity and functional variety. With the intention to test if RNA editing plays a role in pathological processes, which contribute to seizure maintenance, we examined the ratio of the unedited (Q) to edited (R) form o...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2001-06-01
|
| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996101903948 |
| _version_ | 1818721224949235712 |
|---|---|
| author | G. Kortenbruck E. Berger E.-J. Speckmann U. Musshoff |
| author_facet | G. Kortenbruck E. Berger E.-J. Speckmann U. Musshoff |
| author_sort | G. Kortenbruck |
| collection | DOAJ |
| description | Posttranscriptional editing of mRNA is a phenomenon that generates molecular heterogeneity and functional variety. With the intention to test if RNA editing plays a role in pathological processes, which contribute to seizure maintenance, we examined the ratio of the unedited (Q) to edited (R) form of the AMPA receptor subunit GluR2 and kainate receptor subunits GluR5 and GluR6 in the hippocampus and temporal cerebral cortex, both excised from patients with pharmacoresistant temporal lobe epilepsies. We compared the data with samples from nonepileptic human control tissue (autopsy tissue). The ratio of Q/R editing was analyzed by means of reverse transcription-polymerase chain reaction followed by a restriction enzyme assay. We found that the editing efficiency for the kainate receptor subunits GluR5 and GluR6 was significantly higher in temporal cortex than in normal controls. The alteration in GluR5 and GluR6 mRNA editing in the neocortical tissue may reflect an adaptive reaction of ongoing seizure activity to prevent excessive Ca2+ influx. |
| first_indexed | 2024-12-17T20:35:20Z |
| format | Article |
| id | doaj.art-f1bf46946415422398977598d8ee30b0 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-17T20:35:20Z |
| publishDate | 2001-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-f1bf46946415422398977598d8ee30b02022-12-21T21:33:28ZengElsevierNeurobiology of Disease1095-953X2001-06-0183459468RNA Editing at the Q/R Site for the Glutamate Receptor Subunits GLUR2, GLUR5, and GLUR6 in Hippocampus and Temporal Cortex from Epileptic PatientsG. Kortenbruck0E. Berger1E.-J. Speckmann2U. Musshoff3Institute of Physiology, University Münster, Robert-Koch-Strasse 27a, 48149, Münster, GermanyInstitute of Physiology, University Münster, Robert-Koch-Strasse 27a, 48149, Münster, GermanyInstitute of Physiology, University Münster, Robert-Koch-Strasse 27a, 48149, Münster, GermanyInstitute of Physiology, University Münster, Robert-Koch-Strasse 27a, 48149, Münster, GermanyPosttranscriptional editing of mRNA is a phenomenon that generates molecular heterogeneity and functional variety. With the intention to test if RNA editing plays a role in pathological processes, which contribute to seizure maintenance, we examined the ratio of the unedited (Q) to edited (R) form of the AMPA receptor subunit GluR2 and kainate receptor subunits GluR5 and GluR6 in the hippocampus and temporal cerebral cortex, both excised from patients with pharmacoresistant temporal lobe epilepsies. We compared the data with samples from nonepileptic human control tissue (autopsy tissue). The ratio of Q/R editing was analyzed by means of reverse transcription-polymerase chain reaction followed by a restriction enzyme assay. We found that the editing efficiency for the kainate receptor subunits GluR5 and GluR6 was significantly higher in temporal cortex than in normal controls. The alteration in GluR5 and GluR6 mRNA editing in the neocortical tissue may reflect an adaptive reaction of ongoing seizure activity to prevent excessive Ca2+ influx.http://www.sciencedirect.com/science/article/pii/S0969996101903948AMPA receptorkainate receptorRNA editingQ/R sitehuman braintemporal lobe epilepsy |
| spellingShingle | G. Kortenbruck E. Berger E.-J. Speckmann U. Musshoff RNA Editing at the Q/R Site for the Glutamate Receptor Subunits GLUR2, GLUR5, and GLUR6 in Hippocampus and Temporal Cortex from Epileptic Patients Neurobiology of Disease AMPA receptor kainate receptor RNA editing Q/R site human brain temporal lobe epilepsy |
| title | RNA Editing at the Q/R Site for the Glutamate Receptor Subunits GLUR2, GLUR5, and GLUR6 in Hippocampus and Temporal Cortex from Epileptic Patients |
| title_full | RNA Editing at the Q/R Site for the Glutamate Receptor Subunits GLUR2, GLUR5, and GLUR6 in Hippocampus and Temporal Cortex from Epileptic Patients |
| title_fullStr | RNA Editing at the Q/R Site for the Glutamate Receptor Subunits GLUR2, GLUR5, and GLUR6 in Hippocampus and Temporal Cortex from Epileptic Patients |
| title_full_unstemmed | RNA Editing at the Q/R Site for the Glutamate Receptor Subunits GLUR2, GLUR5, and GLUR6 in Hippocampus and Temporal Cortex from Epileptic Patients |
| title_short | RNA Editing at the Q/R Site for the Glutamate Receptor Subunits GLUR2, GLUR5, and GLUR6 in Hippocampus and Temporal Cortex from Epileptic Patients |
| title_sort | rna editing at the q r site for the glutamate receptor subunits glur2 glur5 and glur6 in hippocampus and temporal cortex from epileptic patients |
| topic | AMPA receptor kainate receptor RNA editing Q/R site human brain temporal lobe epilepsy |
| url | http://www.sciencedirect.com/science/article/pii/S0969996101903948 |
| work_keys_str_mv | AT gkortenbruck rnaeditingattheqrsitefortheglutamatereceptorsubunitsglur2glur5andglur6inhippocampusandtemporalcortexfromepilepticpatients AT eberger rnaeditingattheqrsitefortheglutamatereceptorsubunitsglur2glur5andglur6inhippocampusandtemporalcortexfromepilepticpatients AT ejspeckmann rnaeditingattheqrsitefortheglutamatereceptorsubunitsglur2glur5andglur6inhippocampusandtemporalcortexfromepilepticpatients AT umusshoff rnaeditingattheqrsitefortheglutamatereceptorsubunitsglur2glur5andglur6inhippocampusandtemporalcortexfromepilepticpatients |