Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window
Gold nanoparticles have been indicated for use in a diagnostic and/or therapeutic role in several cancer types. The use of gold nanorods (AuNRs) with a surface plasmon resonance (SPR) in the second near-infrared II (NIR-II) optical window promises deeper anatomical penetration through increased maxi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/10/1651 |
| _version_ | 1797513452644728832 |
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| author | Teklu Egnuni Nicola Ingram Ibrahim Mirza P. Louise Coletta James R. McLaughlan |
| author_facet | Teklu Egnuni Nicola Ingram Ibrahim Mirza P. Louise Coletta James R. McLaughlan |
| author_sort | Teklu Egnuni |
| collection | DOAJ |
| description | Gold nanoparticles have been indicated for use in a diagnostic and/or therapeutic role in several cancer types. The use of gold nanorods (AuNRs) with a surface plasmon resonance (SPR) in the second near-infrared II (NIR-II) optical window promises deeper anatomical penetration through increased maximum permissible exposure and lower optical attenuation. In this study, the targeting and therapeutic efficiency of anti-epidermal growth factor receptor (EGFR)-antibody-functionalised AuNRs with an SPR at 1064 nm was evaluated in vitro. Four cell lines, KYSE-30, CAL-27, Hep-G2 and MCF-7, which either over- or under-expressed EGFR, were used once confirmed by flow cytometry and immunofluorescence. Optical microscopy demonstrated a significant difference (<i>p</i> < 0.0001) between targeted AuNRs (tAuNRs) and untargeted AuNRs (uAuNRs) in all four cancer cell lines. This study demonstrated that anti-EGFR functionalisation significantly increased the association of tAuNRs with each EGFR-positive cancer cell. Considering this, the MTT assay showed that photothermal therapy (PTT) significantly increased cancer cell death (>97%) in head and neck cancer cell line CAL-27 using tAuNRs but not uAuNRs, apoptosis being the major mechanism of cell death. This successful targeting and therapeutic outcome highlight the future use of tAuNRs for molecular photoacoustic imaging or tumour treatment through plasmonic photothermal therapy. |
| first_indexed | 2024-03-10T06:16:47Z |
| format | Article |
| id | doaj.art-f1c248e9916e45148bab3337cc0dc368 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T06:16:47Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-f1c248e9916e45148bab3337cc0dc3682023-11-22T19:39:10ZengMDPI AGPharmaceutics1999-49232021-10-011310165110.3390/pharmaceutics13101651Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical WindowTeklu Egnuni0Nicola Ingram1Ibrahim Mirza2P. Louise Coletta3James R. McLaughlan4Leeds Institute of Medical Research, University of Leeds, St. James’s University Hospital, Leeds LS9 7TF, UKLeeds Institute of Medical Research, University of Leeds, St. James’s University Hospital, Leeds LS9 7TF, UKSchool of Electronic and Electrical Engineering, University of Leeds, Leeds LS2 9JT, UKLeeds Institute of Medical Research, University of Leeds, St. James’s University Hospital, Leeds LS9 7TF, UKLeeds Institute of Medical Research, University of Leeds, St. James’s University Hospital, Leeds LS9 7TF, UKGold nanoparticles have been indicated for use in a diagnostic and/or therapeutic role in several cancer types. The use of gold nanorods (AuNRs) with a surface plasmon resonance (SPR) in the second near-infrared II (NIR-II) optical window promises deeper anatomical penetration through increased maximum permissible exposure and lower optical attenuation. In this study, the targeting and therapeutic efficiency of anti-epidermal growth factor receptor (EGFR)-antibody-functionalised AuNRs with an SPR at 1064 nm was evaluated in vitro. Four cell lines, KYSE-30, CAL-27, Hep-G2 and MCF-7, which either over- or under-expressed EGFR, were used once confirmed by flow cytometry and immunofluorescence. Optical microscopy demonstrated a significant difference (<i>p</i> < 0.0001) between targeted AuNRs (tAuNRs) and untargeted AuNRs (uAuNRs) in all four cancer cell lines. This study demonstrated that anti-EGFR functionalisation significantly increased the association of tAuNRs with each EGFR-positive cancer cell. Considering this, the MTT assay showed that photothermal therapy (PTT) significantly increased cancer cell death (>97%) in head and neck cancer cell line CAL-27 using tAuNRs but not uAuNRs, apoptosis being the major mechanism of cell death. This successful targeting and therapeutic outcome highlight the future use of tAuNRs for molecular photoacoustic imaging or tumour treatment through plasmonic photothermal therapy.https://www.mdpi.com/1999-4923/13/10/1651gold nanorodstargetingEGFRHNSCCuptakePTT |
| spellingShingle | Teklu Egnuni Nicola Ingram Ibrahim Mirza P. Louise Coletta James R. McLaughlan Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window Pharmaceutics gold nanorods targeting EGFR HNSCC uptake PTT |
| title | Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window |
| title_full | Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window |
| title_fullStr | Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window |
| title_full_unstemmed | Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window |
| title_short | Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window |
| title_sort | evaluation of the targeting and therapeutic efficiency of anti egfr functionalised nanoparticles in head and neck cancer cells for use in nir ii optical window |
| topic | gold nanorods targeting EGFR HNSCC uptake PTT |
| url | https://www.mdpi.com/1999-4923/13/10/1651 |
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