Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model
Escherichia coli O157:H7 variants were examined for trait mutations and by molecular subtyping to better define clonal complexes postulated on the O157:H7 evolution model. Strains of β-glucuronidase–positive, sorbitol-negative O157:H7 isolated in United States and Japan were identical to A5 clonal s...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2007-11-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/13/11/07-0381_article |
| _version_ | 1818441470242193408 |
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| author | Peter C.H. Feng Steven R. Monday David W. Lacher Lesley Allison Anja Siitonen Christine Keys Marjut Eklund Hideki Nagano Helge Karch James Keen Thomas S. Whittam |
| author_facet | Peter C.H. Feng Steven R. Monday David W. Lacher Lesley Allison Anja Siitonen Christine Keys Marjut Eklund Hideki Nagano Helge Karch James Keen Thomas S. Whittam |
| author_sort | Peter C.H. Feng |
| collection | DOAJ |
| description | Escherichia coli O157:H7 variants were examined for trait mutations and by molecular subtyping to better define clonal complexes postulated on the O157:H7 evolution model. Strains of β-glucuronidase–positive, sorbitol-negative O157:H7 isolated in United States and Japan were identical to A5 clonal strain and shared sequence type (ST)–65 by multilocus sequence typing (MLST); thus, they belong in A5. However, these strains exhibited pulsed-field gel electrophoresis (PFGE) profile differences that suggested genomic divergence between populations. Sorbitol-fermenting O157 (SFO157) strains from Finland, Scotland, and Germany were identical to A4 clonal strain and belong in A4. Some SFO157 strains, isolated years apart and from different countries, had identical PFGE profiles, suggesting a common origin. Despite similarities, some Finnish and Scottish and all of the German strains have ST-75 (“German clone”), whereas others have ST-76, a new variant (“Scottish clone”). MLST of strains in other clonal complexes also discriminated strains thought to be identical and showed that genetic differences will further distinguish clonal populations into subclones. |
| first_indexed | 2024-12-14T18:28:46Z |
| format | Article |
| id | doaj.art-f1c3305a11ac4e978ce429dc1d5602b6 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-14T18:28:46Z |
| publishDate | 2007-11-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-f1c3305a11ac4e978ce429dc1d5602b62022-12-21T22:51:52ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592007-11-0113111701170610.3201/eid1311.070381Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary ModelPeter C.H. FengSteven R. MondayDavid W. LacherLesley AllisonAnja SiitonenChristine KeysMarjut EklundHideki NaganoHelge KarchJames KeenThomas S. WhittamEscherichia coli O157:H7 variants were examined for trait mutations and by molecular subtyping to better define clonal complexes postulated on the O157:H7 evolution model. Strains of β-glucuronidase–positive, sorbitol-negative O157:H7 isolated in United States and Japan were identical to A5 clonal strain and shared sequence type (ST)–65 by multilocus sequence typing (MLST); thus, they belong in A5. However, these strains exhibited pulsed-field gel electrophoresis (PFGE) profile differences that suggested genomic divergence between populations. Sorbitol-fermenting O157 (SFO157) strains from Finland, Scotland, and Germany were identical to A4 clonal strain and belong in A4. Some SFO157 strains, isolated years apart and from different countries, had identical PFGE profiles, suggesting a common origin. Despite similarities, some Finnish and Scottish and all of the German strains have ST-75 (“German clone”), whereas others have ST-76, a new variant (“Scottish clone”). MLST of strains in other clonal complexes also discriminated strains thought to be identical and showed that genetic differences will further distinguish clonal populations into subclones.https://wwwnc.cdc.gov/eid/article/13/11/07-0381_articleEnterohemorrhagic E. coli O157:H7clonal complexesgenetic diversitymolecular evolutionresearchUnited States |
| spellingShingle | Peter C.H. Feng Steven R. Monday David W. Lacher Lesley Allison Anja Siitonen Christine Keys Marjut Eklund Hideki Nagano Helge Karch James Keen Thomas S. Whittam Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model Emerging Infectious Diseases Enterohemorrhagic E. coli O157:H7 clonal complexes genetic diversity molecular evolution research United States |
| title | Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model |
| title_full | Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model |
| title_fullStr | Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model |
| title_full_unstemmed | Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model |
| title_short | Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model |
| title_sort | genetic diversity among clonal lineages within escherichia coli o157 h7 stepwise evolutionary model |
| topic | Enterohemorrhagic E. coli O157:H7 clonal complexes genetic diversity molecular evolution research United States |
| url | https://wwwnc.cdc.gov/eid/article/13/11/07-0381_article |
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