Detection of <i>Wolbachia</i> Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, <i>Mayetiola destructor</i> (Say)
<i>Mayetiola destructor</i> (Hessian fly) is a destructive pest of wheat in several parts of the world. Here, we investigated the presence of reproductive symbionts and the effect of the geographical location on the bacterial community associated to adult Hessian flies derived from four...
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MDPI AG
2020-06-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/6/340 |
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| author | Naima Bel Mokhtar Amal Maurady Mohammed Reda Britel Mustapha El Bouhssini Costas Batargias Panagiota Stathopoulou Elias Asimakis George Tsiamis |
| author_facet | Naima Bel Mokhtar Amal Maurady Mohammed Reda Britel Mustapha El Bouhssini Costas Batargias Panagiota Stathopoulou Elias Asimakis George Tsiamis |
| author_sort | Naima Bel Mokhtar |
| collection | DOAJ |
| description | <i>Mayetiola destructor</i> (Hessian fly) is a destructive pest of wheat in several parts of the world. Here, we investigated the presence of reproductive symbionts and the effect of the geographical location on the bacterial community associated to adult Hessian flies derived from four major wheat producing areas in Morocco. Using specific 16S rDNA PCR assay, <i>Wolbachia</i> infection was observed in 3% of the natural populations and 10% of the laboratory population. High throughput sequencing of V3-V4 region of the bacterial 16S rRNA gene revealed that the microbiota of adult Hessian flies was significantly influenced by their native regions. A total of 6 phyla, 10 classes and 79 genera were obtained from all the samples. Confirming the screening results, <i>Wolbachia</i> was identified as well in the natural Hessian flies. Phylogenetic analysis using the sequences obtained in this study indicated that there is one <i>Wolbachia</i> strain belonging to supergroup A. To our knowledge, this is the first report of <i>Wolbachia</i> in Hessian fly populations. The observed low abundance of <i>Wolbachia</i> most likely does not indicate induction of reproductive incompatibility. Yet, this infection may give a new insight into the use of <i>Wolbachia</i> for the fight against Hessian fly populations. |
| first_indexed | 2024-03-10T19:26:18Z |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T19:26:18Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-f1c43cb498f144e59354143fdca0d5b32023-11-20T02:34:39ZengMDPI AGInsects2075-44502020-06-0111634010.3390/insects11060340Detection of <i>Wolbachia</i> Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, <i>Mayetiola destructor</i> (Say)Naima Bel Mokhtar0Amal Maurady1Mohammed Reda Britel2Mustapha El Bouhssini3Costas Batargias4Panagiota Stathopoulou5Elias Asimakis6George Tsiamis7Laboratory of Innovative Technologies, National School of Applied Sciences of Tangier, Abdelmalek Essaâdi University, BP: 1818 Tanger, MoroccoLaboratory of Innovative Technologies, National School of Applied Sciences of Tangier, Abdelmalek Essaâdi University, BP: 1818 Tanger, MoroccoLaboratory of Innovative Technologies, National School of Applied Sciences of Tangier, Abdelmalek Essaâdi University, BP: 1818 Tanger, MoroccoInternational Center for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 6299 Rabat, MoroccoDepartment of Animal Production, Fisheries and Aquaculture, University of Patras, Nea Ktiria, 30200 Messologhi, GreeceDepartment of Environmental Engineering, University of Patras, 2 Seferi St, 30100 Agrinio, GreeceDepartment of Environmental Engineering, University of Patras, 2 Seferi St, 30100 Agrinio, GreeceDepartment of Environmental Engineering, University of Patras, 2 Seferi St, 30100 Agrinio, Greece<i>Mayetiola destructor</i> (Hessian fly) is a destructive pest of wheat in several parts of the world. Here, we investigated the presence of reproductive symbionts and the effect of the geographical location on the bacterial community associated to adult Hessian flies derived from four major wheat producing areas in Morocco. Using specific 16S rDNA PCR assay, <i>Wolbachia</i> infection was observed in 3% of the natural populations and 10% of the laboratory population. High throughput sequencing of V3-V4 region of the bacterial 16S rRNA gene revealed that the microbiota of adult Hessian flies was significantly influenced by their native regions. A total of 6 phyla, 10 classes and 79 genera were obtained from all the samples. Confirming the screening results, <i>Wolbachia</i> was identified as well in the natural Hessian flies. Phylogenetic analysis using the sequences obtained in this study indicated that there is one <i>Wolbachia</i> strain belonging to supergroup A. To our knowledge, this is the first report of <i>Wolbachia</i> in Hessian fly populations. The observed low abundance of <i>Wolbachia</i> most likely does not indicate induction of reproductive incompatibility. Yet, this infection may give a new insight into the use of <i>Wolbachia</i> for the fight against Hessian fly populations.https://www.mdpi.com/2075-4450/11/6/340biological controlHessian flyhigh throughput sequencingHTS16S rRNA gene |
| spellingShingle | Naima Bel Mokhtar Amal Maurady Mohammed Reda Britel Mustapha El Bouhssini Costas Batargias Panagiota Stathopoulou Elias Asimakis George Tsiamis Detection of <i>Wolbachia</i> Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, <i>Mayetiola destructor</i> (Say) Insects biological control Hessian fly high throughput sequencing HTS 16S rRNA gene |
| title | Detection of <i>Wolbachia</i> Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, <i>Mayetiola destructor</i> (Say) |
| title_full | Detection of <i>Wolbachia</i> Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, <i>Mayetiola destructor</i> (Say) |
| title_fullStr | Detection of <i>Wolbachia</i> Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, <i>Mayetiola destructor</i> (Say) |
| title_full_unstemmed | Detection of <i>Wolbachia</i> Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, <i>Mayetiola destructor</i> (Say) |
| title_short | Detection of <i>Wolbachia</i> Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, <i>Mayetiola destructor</i> (Say) |
| title_sort | detection of i wolbachia i infections in natural and laboratory populations of the moroccan hessian fly i mayetiola destructor i say |
| topic | biological control Hessian fly high throughput sequencing HTS 16S rRNA gene |
| url | https://www.mdpi.com/2075-4450/11/6/340 |
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