Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol
Lipase activity was detected based on the fluorescence properties of gold nanoclusters (AuNCs) and the inner filter effect (IFE) of p-nitrophenol. Glutathione-modified AuNCs was used as fluorophore, and p-nitrophenol produced from the lipase-catalyzed hydrolysis of p-nitrophenol palmitate as fluores...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-07-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-14-013.pdf |
| _version_ | 1797742565969100800 |
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| author | XU Yang, XIN Jiaying, LI Huimin, WANG Guiru, WANG Yuqing |
| author_facet | XU Yang, XIN Jiaying, LI Huimin, WANG Guiru, WANG Yuqing |
| author_sort | XU Yang, XIN Jiaying, LI Huimin, WANG Guiru, WANG Yuqing |
| collection | DOAJ |
| description | Lipase activity was detected based on the fluorescence properties of gold nanoclusters (AuNCs) and the inner filter effect (IFE) of p-nitrophenol. Glutathione-modified AuNCs was used as fluorophore, and p-nitrophenol produced from the lipase-catalyzed hydrolysis of p-nitrophenol palmitate as fluorescence absorbent. The results showed that under the optimal conditions (p-nitrophenyl palmitate concentration 1.6 mg/mL, pH 7.5, temperature 50 ℃, and reaction time 20 min), the relative fluorescence intensity (F0–F, y) was positively correlated with lipase activity (x) in the range of 5.6–196 U/L, which was described by the equation y = 2.003 5 + 0.936 8x, with a correlation coefficient (r2) of 0.997 8, and the detection limit was 1.3 U/L (at a signal-to-noise ratio of 3). The developed method is simple and sensitive, and can be applied to the detection of lipase activity. |
| first_indexed | 2024-03-12T14:42:31Z |
| format | Article |
| id | doaj.art-f1c5238c35794414a661874e1fcb4c42 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-12T14:42:31Z |
| publishDate | 2023-07-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-f1c5238c35794414a661874e1fcb4c422023-08-16T05:39:29ZengChina Food Publishing CompanyShipin Kexue1002-66302023-07-01441410010510.7506/spkx1002-6630-20220815-169Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-NitrophenolXU Yang, XIN Jiaying, LI Huimin, WANG Guiru, WANG Yuqing0(1. Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China;2. State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences, Lanzhou 730000, China)Lipase activity was detected based on the fluorescence properties of gold nanoclusters (AuNCs) and the inner filter effect (IFE) of p-nitrophenol. Glutathione-modified AuNCs was used as fluorophore, and p-nitrophenol produced from the lipase-catalyzed hydrolysis of p-nitrophenol palmitate as fluorescence absorbent. The results showed that under the optimal conditions (p-nitrophenyl palmitate concentration 1.6 mg/mL, pH 7.5, temperature 50 ℃, and reaction time 20 min), the relative fluorescence intensity (F0–F, y) was positively correlated with lipase activity (x) in the range of 5.6–196 U/L, which was described by the equation y = 2.003 5 + 0.936 8x, with a correlation coefficient (r2) of 0.997 8, and the detection limit was 1.3 U/L (at a signal-to-noise ratio of 3). The developed method is simple and sensitive, and can be applied to the detection of lipase activity.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-14-013.pdflipase activity; gold nanocluster; fluorescence inner filter effect; optical properties; detection |
| spellingShingle | XU Yang, XIN Jiaying, LI Huimin, WANG Guiru, WANG Yuqing Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol Shipin Kexue lipase activity; gold nanocluster; fluorescence inner filter effect; optical properties; detection |
| title | Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol |
| title_full | Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol |
| title_fullStr | Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol |
| title_full_unstemmed | Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol |
| title_short | Detection of Lipase Activity Based on Fluorescence Inner Filter Effect of p-Nitrophenol |
| title_sort | detection of lipase activity based on fluorescence inner filter effect of p nitrophenol |
| topic | lipase activity; gold nanocluster; fluorescence inner filter effect; optical properties; detection |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-14-013.pdf |
| work_keys_str_mv | AT xuyangxinjiayinglihuiminwangguiruwangyuqing detectionoflipaseactivitybasedonfluorescenceinnerfiltereffectofpnitrophenol |